PslD Is a Secreted Protein Required for Biofilm Formation by
            <i>Pseudomonas aeruginosa</i>

Campisano, A.; Schroeder, Christine; Schemionek, Mirle; Overhage, J. Marc; Rehm, Bernd H. A.

doi:10.1128/aem.72.4.3066-3068.2006

Cited by 45 publications

(39 citation statements)

References 22 publications

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“…This is an area requiring future research. Overhage and Campisano et al demonstrated that pslA and pslD, which encode a putative UDP-glucose lipid carrier and exporter, respectively, are essential for biofilm formation in strain PAO1 [23,24]. They also demonstrated that while psl is constitutively expressed in planktonic cells, its expression is localized to the center of developing biofilm microcolonies [24].…”

Section: Pslmentioning

confidence: 97%

Role of polysaccharides in Pseudomonas aeruginosa biofilm development

Ryder

Byrd

Wozniak

2007

Current Opinion in Microbiology

509

385

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During the past decade, there has been a renewed interest in using P. aeruginosa as a model system for biofilm development and pathogenesis. Since the biofilm matrix represents a critical interface between the bacterium and the host or its environment, considerable effort has been expended to acquire a more complete understanding of the matrix composition. Here, we focus on recent developments regarding the roles of alginate, Psl, and Pel polysaccharides in the biofilm matrix.

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Section: Pslmentioning

confidence: 97%

Role of polysaccharides in Pseudomonas aeruginosa biofilm development

Ryder

Byrd

Wozniak

2007

Current Opinion in Microbiology

509

385

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“…4b) and are predicted to be a mixture of lipidated and nonlipidated representatives. PslD is an outlier located between OPX groups C and D. It is involved in the export of an EPS in Pseudomonas aeruginosa that is required for biofilm formation (14,96). Group D OPX proteins are heterogeneous with respect to size and lipidation.…”

Section: Structures and Phylogeny Of Opx Proteinsmentioning

confidence: 99%

Pivotal Roles of the Outer Membrane Polysaccharide Export and Polysaccharide Copolymerase Protein Families in Export of Extracellular Polysaccharides in Gram-Negative Bacteria

Cuthbertson

Mainprize

Naismith

et al. 2009

Microbiol Mol Biol Rev

257

290

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SUMMARY Many bacteria export extracellular polysaccharides (EPS) and capsular polysaccharides (CPS). These polymers exhibit remarkably diverse structures and play important roles in the biology of free-living, commensal, and pathogenic bacteria. EPS and CPS production represents a major challenge because these high-molecular-weight hydrophilic polymers must be assembled and exported in a process spanning the envelope, without compromising the essential barrier properties of the envelope. Emerging evidence points to the existence of molecular scaffolds that perform these critical polymer-trafficking functions. Two major pathways with different polymer biosynthesis strategies are involved in the assembly of most EPS/CPS: the Wzy-dependent and ATP-binding cassette (ABC) transporter-dependent pathways. They converge in an outer membrane export step mediated by a member of the outer membrane auxiliary (OMA) protein family. OMA proteins form outer membrane efflux channels for the polymers, and here we propose the revised name outer membrane polysaccharide export (OPX) proteins. Proteins in the polysaccharide copolymerase (PCP) family have been implicated in several aspects of polymer biogenesis, but there is unequivocal evidence for some systems that PCP and OPX proteins interact to form a trans-envelope scaffold for polymer export. Understanding of the precise functions of the OPX and PCP proteins has been advanced by recent findings from biochemistry and structural biology approaches and by parallel studies of other macromolecular trafficking events. Phylogenetic analyses reported here also contribute important new insight into the distribution, structural relationships, and function of the OPX and PCP proteins. This review is intended as an update on progress in this important area of microbial cell biology.

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“…The psl gene cluster contains 15 cotranscribed genes (pslA to pslO) encoding proteins predicted to synthesize the Psl EPS, which is important to initiate and maintain biofilm structure by providing cell-cell and cell-surface interactions (3,5,8,13,15). In the present study, we used a Psl-inducible P. aeruginosa strain, WFPA801 (⌬psl p BAD -psl), and several psl mutants to study the chemical composition of the Psl EPS.…”

mentioning

confidence: 99%