Psf3 is a prognostic biomarker in lung adenocarcinoma: a larger trial using tissue microarrays of 864 consecutive resections

Tauchi, Shunsuke; Sakai, Yasuhiro; Fujimoto, Shuntaro; Ogawa, Hiroyuki; Tane, Shinya; Hokka, Daisuke; Tanaka, Yugo; Nishio, Wataru; Yoshimura, Masahiro; Yanagita, Emmy; Itoh, Tomoo; Hayashi, Yoshitake; Maniwa, Yoshimasa

doi:10.1093/ejcts/ezw077

Cited by 11 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the Psf3 expression was determined using a microarray as described in previous studies . The cutoff value was 50% staining.…”

Section: Methodsmentioning

confidence: 99%

“…Partner of Sld five 3 (Psf3) is one of the proteins known to comprise Go‐Ichi‐Ni‐San (GINS), which is a macromolecular protein complex associated with DNA replication. Although our previous studies have shown that Psf3 expression worked as a prognostic indicator in pulmonary adenocarcinoma, its relationship with the efficacy of adjuvant chemotherapy was not determined.…”

Section: Introductionmentioning

confidence: 92%

“…Tissue microarrays and immunohistochemistry were performed as previously described. 9 For the microarrays, tissue sections taken from the resected specimens that represented the largest cleaved surface of the tumor mass were cut into small circles. Plural circular sections were placed on a single slide, deparaffinized with xylene, and rehydrated with ethanol.…”

Section: Tissue Microarray and Immunohistochemistrymentioning

confidence: 99%

“…In this study, the Psf3 expression was determined using a microarray as described in previous studies. 9 The cutoff value was 50% staining. If more than 50% of cancer cells in the microarray tissue samples showed nuclear staining and exhibited a clustering pattern of Psf3 in the microscope area (×200), the tissue was classified as high expression of Psf3; if not, the tissue was classified as low expression of Psf3.…”

Section: Classification Of Immunohistochemical Staining Patternsmentioning

confidence: 99%

See 3 more Smart Citations

Psf3 as a possible biomarker of postoperative chemotherapy for patients with early pulmonary adenocarcinoma

et al. 2019

Self Cite

View full text Add to dashboard Cite

BackgroundPartner of Sld five 3 (Psf3) is a member of the heterotetrameric complex that consists of SLD5, Psf1, Psf2, and Psf3. We have shown in previous studies that high Psf3 expression was a poor prognostic marker for pulmonary adenocarcinoma. Here, we statistically evaluated the relationship between clinicopathologic factors and Psf3 expression in stage I pulmonary adenocarcinoma.MethodsA total of 583 patients who had undergone complete resection of stage I pulmonary adenocarcinoma from January 2002 to December 2009 were included in the study. Tissue microarrays were performed, and the resected tumors were divided into groups according to Psf3 expression.ResultsOf 583 patients, high expression of Psf3 was observed in 211 (36.2%) and low expression of Psf3 observed in 372 (63.8%) patients. Among stage I patients, the five‐year survival rate was 76.7% in the Psf3 high expression group and 90.9% in the Psf3 low expression group (P < 0.0001). On multivariate analysis, Psf3 was found to be the independent prognostic factor. Among stage I patients in the Psf3 high expression group, a significantly greater five‐year survival rate was observed in patients who received postoperative chemotherapy with tegafur‐uracil than in those who underwent surgery alone (P < 0.0001). In contrast, among stage I patients in the Psf3 low expression group, no difference was found in the five‐year survival, regardless of the presence or absence of tegafur‐uracil (P = 0.873).ConclusionThe Psf3 expression was an independent prognostic factor and could be a biomarker of adjuvant tegafur‐uracil for stage I pulmonary adenocarcinoma.Key points Significant findings of the study: The Psf3 expression could be a biomarker of adjuvant tegafur‐uracil administration for stage I pulmonary adenocarcinoma. What this study adds: Appropriate patients of adjuvant chemotherapy for stage I pulmonary adenocarcinoma using Psf3 expression could be selected.

show abstract

“…In this study, the Psf3 expression was determined using a microarray as described in previous studies . The cutoff value was 50% staining.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Section: Tissue Microarray and Immunohistochemistrymentioning

confidence: 99%

Section: Classification Of Immunohistochemical Staining Patternsmentioning

confidence: 99%

See 2 more Smart Citations

Psf3 as a possible biomarker of postoperative chemotherapy for patients with early pulmonary adenocarcinoma

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In healthy humans, higher expression of GINS orthologs is detected in proliferative tissues relative to differentiated cells (Uhlén et al, 2015). Enhanced expression of GINS components is also reported in numerous cancer types, and these genes can serve as important biomarkers for cancer therapy (Kanzaki et al, 2016;Tauchi et al, 2016;Yamane et al, 2016). In several cancer cell lines, inhibiting GINS function results in a decrease in proliferation and invasive behaviors, suggesting that the complex may be an important therapeutic target (Liang et al, 2016;Yamane et al, 2016;Zhang et al, 2015;Zhang et al, 2013).…”

mentioning

confidence: 99%

The GINS complex is required for the survival of rapidly proliferating retinal and tectal progenitor cells during zebrafish development

Varga

Csályi

Bertyák

et al. 2020

Preprint

View full text Add to dashboard Cite

Efficient and accurate DNA replication is particularly critical in stem and progenitor cells for successful proliferation and survival. The replisome, an amalgam of protein complexes, is responsible for binding potential origins of replication, unwinding the double helix, and then synthesizing complimentary strands of DNA. According to current models, the initial steps of DNA unwinding and opening are facilitated by the CMG complex, which is composed of a GINS heterotetramer that connects Cdc45 with the mini-chromosome maintenance (Mcm) helicase. In this work, we provide evidence that in the absence of GINS function DNA replication is cell autonomously impaired, and we also show that gins1 and gins2 mutants exhibit elevated levels of apoptosis restricted to actively proliferating regions of the central nervous system (CNS). Intriguingly, our results also suggest that the rapid cell cycles during early embryonic development in zebrafish may not require the function of the GINS complex as neither Gins1 nor Gins2 seem to be present during these stages.

show abstract

GINS2 promotes cell proliferation and inhibits cell apoptosis in thyroid cancer by regulating CITED2 and LOXL2

Song

et al. 2018

Cancer Gene Ther

View full text Add to dashboard Cite

To explore the mechanisms of GINS2 on cell proliferation and apoptosis in thyroid cancer (TC) cells. Expressions of GINS2 were inhibited in K1 and SW579 cells using gene interference technology. The abilities of proliferation and apoptosis, and cell cycle were determined by MTT assay and flow cytometric assay. The downstream molecules of GINS2 were searched by microarray and bioinformatics and validated by qRT-PCR and western blotting. In the in vivo study, the tumor growth was compared and the whole-body fluorescent imaging was analyzed. After GINS2 was interfered, cell proliferation was significantly inhibited (P < 0.01) and apoptosis rate increased (P < 0.01) in both K1 and SW579 cells. Cell cycle changed significantly in K1 cells, but not in SW579 cells. With bioinformatics upstream analysis, TGF-β1 was found as the most significantly upstream regulator. Expressions of TGF-β1 and its downstream target molecules CITED2 and LOXL2 were validated and found downregulated significantly in mRNA and protein levels (P < 0.05). The results of the nude mouse xenograft assay suggested that the volume and weight of tumor in ones infected with shGINS2 were statistically smaller than controls (P < 0.05). GINS2 plays an important role in cell proliferation and apoptosis of thyroid cancer by regulating the expressions of CITED2 and LOXL2, which may be a potential biomarker for diagnosis or prognosis and a drug target for therapy.

show abstract

Psf3 is a prognostic biomarker in lung adenocarcinoma: a larger trial using tissue microarrays of 864 consecutive resections

Abstract: The present findings strengthened our previous data demonstrating that high Psf3 expression in primary lung adenocarcinoma plays an important role in disease progression and is a prognostic indicator, particularly in early-stage adenocarcinoma.

Cited by 11 publications

References 22 publications

Psf3 as a possible biomarker of postoperative chemotherapy for patients with early pulmonary adenocarcinoma

Psf3 as a possible biomarker of postoperative chemotherapy for patients with early pulmonary adenocarcinoma

The GINS complex is required for the survival of rapidly proliferating retinal and tectal progenitor cells during zebrafish development

GINS2 promotes cell proliferation and inhibits cell apoptosis in thyroid cancer by regulating CITED2 and LOXL2

Contact Info

Product

Resources

About