Psf3 is a prognostic biomarker in lung adenocarcinoma

Hokka, Daisuke; Maniwa, Yoshimasa; Tane, Shinya; Nishio, Wataru; Yoshimura, Masahiro; Okita, Yutaka; Ohbayashi, Chiho; Sakai, Yasuhiro; Chen, Xue; Hayashi, Yoshitake

doi:10.1016/j.lungcan.2012.10.001

Cited by 26 publications

(32 citation statements)

References 22 publications

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“…Recent studies show that several GINS components are highly expressed in different kinds of cancer and related to poor prognosis and tumor progression [9,14,15,11,12].…”

Section: Discussionmentioning

confidence: 99%

“…Several recent reports have suggested that PSF1 is required for acute proliferation of cells, particularly immature cells such as stem cells and progenitor cells and that this protein is also used as a marker for the detection of cancer stem cells [6,8]. Moreover, previous studies have suggested that PSF1 and some other GINS complex members are unregulated in cancer and associated with tumor malignancy characters [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro

Zhang

Wang

et al. 2014

Tumor Biol.

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Partner of sld five 1 (PSF1) is a member of the heterotetrameric complex termed GINS. Previous studies have shown that PSF1 is unregulated in several cancer and associated with tumor malignant characters. However, the effects of PSF1 in lung cancer are still unclear. The goal of this study was to investigate the effects of PSF1 on the proliferation capacities of lung cancer. To start with, expression of PSF1 in 22 human lung cancer samples and adjacent non-tumor samples were detected by real-time RT-PCR and Western blotting. Our results showed that PSF1 was overexpressed in lung cancer samples compared to adjacent non-tumor samples. To achieve better insights of PSF1 functions in lung cancer cells, we used PSF1-specific small interfering RNA (siRNA) successfully inhibit the expression of PSF1 in messenger RNA (mRNA) and protein levels. In addition, we used lung cancer cell lines with different p53 gene background (p53 null and p53 wild-type). The results showed that knockdown of PSF1 inhibited cell proliferation and caused cell cycle arrest of lung cancer cells in a p53-independent manner. Our data indicated that PSF1 is functionally involved in lung cancer cell proliferation and is a potential target for lung cancer therapy.

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“…Recent studies show that several GINS components are highly expressed in different kinds of cancer and related to poor prognosis and tumor progression [9,14,15,11,12].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro

Zhang

Wang

et al. 2014

Tumor Biol.

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“…For the assessment of the protein expression of Sirt1, samples were classified as Sirt1-positive when the ratio of stained cells in all epithelial cancer cells of a tumor tissue was >50%, and as Sirt1-negative if it was <50%. The cut-off value was set at 50%, as this value was statistically useful for this study (24). …”

Section: Methodsmentioning

confidence: 99%

“…IHC was previously performed for 10 cancer-related proteins (including CDC45, HIF1, psf3, E-cadhelin and necl5) with the same paraffin-embedded specimens of the 125 cases investigated in the present study. The correlation between their expression in cancer cells compared with Sirt1 expression was also examined, however, only HIF1 showed a statistically significant different (P<0.05) (24–26). …”

Section: Methodsmentioning

confidence: 99%

Sirt1 is a tumor promoter in lung adenocarcinoma

et al. 2014

Self Cite

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Sirtuin 1 (Sirt1) is a nicotinamide adenine dinucleotide-dependent class III histone deacetylase. It reportedly can repress cellular apoptosis and senescence to affect DNA repair, stress response and aging. Notably, previous data have indicated that Sirt1 is both a tumor promoter and a tumor suppressor in tumorigenesis. However, Sirt1 expression in primary lung adenocarcinoma remains unknown. Immunohistochemical staining was performed to investigate Sirt1 expression in cancer cells in 125 consecutive resected cases of primary lung adenocarcinoma. Sirt1 expression was found to be increased in 26 (20.8%) of the 125 cases, which correlated significantly with five clinicopathological factors: Ki67 index, hypoxia-inducible factor 1 (HIF1) molecule expression, tumor-node-metastasis (TNM) classification, pulmonary vein invasion and lymphatic duct invasion. In the Sirt1-positive expression group, Sirt1 expression correlated with a higher Ki67 index and higher TNM classification, particularly for lymph node invasion and metastasis, and with a higher number of pulmonary vein invasion and lymphatic duct invasion. Additionally, a negative correlation was identified between HIF1-positive expression and Sirt1-negative expression. These results indicate that Sirt1 overexpression plays a promotional role in tumorigenesis and is closely associated with invasion and metastasis and, thus, it may be associated with prognosis.

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“…The advance of these technologies allows molecular diagnostic biomarkers for the detection of lung cancer in addition to computed tomography (CT) screening (4–7). For example, the utility of epidermal growth factor receptor (EGFR) mutation testing is strongly recommended (8) in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Integrative Analysis for Lung Adenocarcinoma Predicts Morphological Features Associated With Genetic Variations

Wang

Yang

et al. 2016

Biocomputing 2017

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Lung cancer is one of the most deadly cancers and lung adenocarcinoma (LUAD) is the most common histological type of lung cancer. However, LUAD is highly heterogeneous due to genetic difference as well as phenotypic differences such as cellular and tissue morphology. In this paper, we systematically examine the relationships between histological features and gene transcription. Specifically, we calculated 283 morphological features from histology images for 201 LUAD patients from TCGA project and identified the morphological feature with strong correlation with patient outcome. We then modeled the morphology feature using multiple co-expressed gene clusters using Lasso-regression. Many of the gene clusters are highly associated with genetic variations, specifically DNA copy number variations, implying that genetic variations play important roles in the development cancer morphology. As far as we know, our finding is the first to directly link the genetic variations and functional genomics to LUAD histology. These observations will lead to new insight on lung cancer development and potential new integrative biomarkers for prediction patient prognosis and response to treatments.

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Psf3 is a prognostic biomarker in lung adenocarcinoma

Cited by 26 publications

References 22 publications

Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro

Knockdown of PSF1 expression inhibits cell proliferation in lung cancer cells in vitro

Sirt1 is a tumor promoter in lung adenocarcinoma

Integrative Analysis for Lung Adenocarcinoma Predicts Morphological Features Associated With Genetic Variations

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