PSF is an IbeA-binding protein contributing to meningitic Escherichia coli K1 invasion of human brain microvascular endothelial cells

Zou, Yanming; He, Liyun; Wu, Chunhua; Cao, Hong; Xie, Zhihua; Ouyang, Yanling; Wang, Yang; Jong, Ambrose; Huang, Sheng‐He

doi:10.1007/s00430-006-0034-x

Cited by 24 publications

(37 citation statements)

References 36 publications

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“…This feature is in accordance with all of the predictions that we obtained in a previous work using several dedicated software programs (9). Nevertheless, it is in contradiction to previous studies assigning to IbeA a direct adhesive function (59,60). To verify our hypothesis, it is now essential that the precise reaction mediated by IbeA be determined.…”

Section: Discussionsupporting

confidence: 87%

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New Role for the ibeA Gene in H2O2 Stress Resistance of Escherichia coli

Fléchard

Cortès

Répérant

et al. 2012

Journal of Bacteriology

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Section: Discussionsupporting

confidence: 87%

“…In the APEC strain BEN2908, the deletion of ibeA led to a significant reduction of virulence (20). So far, the precise function of IbeA has remained elusive and controversial, as some authors suggest it could be an adhesin, while our studies have shown no such role in APEC strain BEN2908 (9,59,60).…”

mentioning

confidence: 52%

New Role for the ibeA Gene in H2O2 Stress Resistance of Escherichia coli

Fléchard

Cortès

Répérant

et al. 2012

Journal of Bacteriology

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“…We also showed that, as in meningitic E. coli strains, IbeA is involved in the virulence of strain BEN2908 for chickens (18). However, the role of IbeA in the virulence of ExPEC remains unclear, despite the characterization of proteins interacting with IbeA in vitro (43,55,56).…”

Section: Discussionmentioning

confidence: 76%

Inactivation of ibeA and ibeT Results in Decreased Expression of Type 1 Fimbriae in Extraintestinal Pathogenic Escherichia coli Strain BEN2908

et al. 2008

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IbeA in extraintestinal pathogenic Escherichia coli (ExPEC) strains was previously described for its role in invasion. Here we investigated the role of IbeA and IbeT, encoded by a gene located downstream of ibeA, in the adhesion of the avian ExPEC strain BEN2908 to human brain microvascular endothelial cells (HBMEC). The ⌬ibeA mutant was less adhesive to HBMEC than the wild-type strain BEN2908 was. Because strain BEN2908 also expresses type 1 fimbriae, we measured the adhesion specifically due to IbeA by comparing the adhesive properties of a ⌬fim derivative of strain BEN2908 to those of a double ⌬fim ⌬ibeA mutant. No differences were observed, indicating that the reduction of adhesion in BEN2908 ⌬ibeA could be due to a decrease in type 1 fimbria expression. We indeed showed that the decreased adhesion of BEN2908 ⌬ibeA was correlated with a decrease in type 1 fimbria expression. Accordingly, more bacteria had a fim promoter orientated in the off position in a culture of BEN2908 ⌬ibeA than in a culture of BEN2908. Expression of fimB and fimE, two genes encoding recombinases participating in controlling the orientation of the fim promoter, was decreased in BEN2908 ⌬ibeA. A reduction of type 1 fimbria expression due to a preferential orientation of the fim promoter in the off position was also seen in an ibeT mutant of strain BEN2908. We finally suggest a role for IbeA and IbeT in modulating the expression of type 1 fimbriae through an as yet unknown mechanism.Extraintestinal pathogenic Escherichia coli (ExPEC) strains are responsible for a wide range of diseases in humans and animals. In humans, almost 80% of urinary tract infections, as well as almost 20% of newborn meningitis cases, are caused by ExPEC strains (23,45). In chickens, ExPEC strains are responsible for avian colibacillosis, which results in different syndromes, ranging from superficial dermatitis to respiratory infections that frequently develop into systemic infections (12,32). Phylogenetic studies have shown that avian ExPEC strains are closely related to human ExPEC strains (40, 44). Additionally, avian ExPEC strains share common virulence genes with ExPEC strains isolated from patients with urinary tract infections or meningitis (16,40,44). Several potential virulence factors have been described for ExPEC strains. These virulence factors confer different properties, such as adhesion to host cells through type 1 and P fimbriae, iron acquisition, toxic activity toward eukaryotic cells (cytotoxins), and resistance to host defenses.ibeA is an ExPEC virulence gene that was first described for strain RS218, isolated from a patient with human newborn meningitis (26,46). ibeA encodes a 50-kDa protein and is located on the GimA genomic island. This island is inserted between yjiD and yjiE and is predicted to contain four operons, three of them potentially involved in carbohydrate metabolism (24). In addition to ibeA, the GimA4 operon is predicted to contain two other genes, ibeR and ibeT. ibeR encodes a potential transcriptional activator homologous to t...

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“…This virulence factor was observed to contribute to the invasion of ExPEC into host cells (18,27). Furthermore, the IbeA receptor PSF was described for both human and bovine BMEC (28,48,67,68), but the precise role of ibeA in the process of infection was still unclear. Thus, this study was conducted to investigate the role of ibeA in APEC.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, IbeA was identified to interact with its receptor, an albumin-like protein, on the surface of both human (45-kDa) and bovine (55-kDa) BMEC. Furthermore, an IbeA-binding protein was purified from intestinal epithelial Caco-2 cells (28,48,67,68). IbeA could therefore be involved in the receptor ligand-mediated invasion of BMEC.…”

mentioning

confidence: 99%

Effects of ibeA Deletion on Virulence and Biofilm Formation of Avian Pathogenic Escherichia coli

et al. 2011

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The ibeA gene is located on a genomic island, GimA, which is involved in the pathogenesis of neonatal meningitis Escherichia coli (NMEC) and avian pathogenic E. coli (APEC). The prevalence of ibeA in the APEC collection in China was investigated, and 20 of 467 strains (4.3%) were positive. In addition, analysis of the association of the E. coli reference (ECOR) groups with positive strains revealed that ibeA was linked to group B2. The ibeA gene in DE205B was analyzed and compared to those of APEC and NMEC, which indicated that the specificity of ibeA was not consistent along pathotypes. The invasion of chicken embryo fibroblast DF-1 cells by APEC DE205B and RS218 was observed, which suggested that DF-1 cells could be a model to study the mechanism of APEC invasion. The inactivation of ibeA in APEC DE205B led to the reduced capacity to invade DF-1 cells, defective virulence in vivo, and decreased biofilm formation compared to the wild-type strain. In addition, strain AAEC189 expressing ibeA exhibited enhanced invasion capacity and biofilm formation. The results of the quantitative real-time reverse transcription-PCR (qRT-PCR) analysis and animal system infection experiments indicated that the loss of ibeA decreased the colonization and proliferation capacities of APEC in the brain during system infection.Escherichia coli typically colonizes the mammalian and avian gastrointestinal tract and other mucosal surfaces. While many of these strains are commensal, certain pathogenic strains can cause severe diseases (33). Extraintestinal pathogenic E. coli (ExPEC) is a group of strains that have been implicated in a large range of infections in humans and animals, such as neonatal meningitis, urinary tract infections (UTIs), pneumonia, osteomyelitis, and septicemia (14,16,30,35). Among these, typical infections caused by ExPEC in humans are UTIs and neonatal meningitis (5). Similarly, systemic infections caused by avian pathogenic E. coli (APEC) are economically devastating to poultry industries (14, 16). APEC enters and colonizes the avian respiratory tract by inhalation of fecal dust, leading to localized infections, such as airsacculitis and pneumonia. In certain cases, they spread into various internal organs, typically causing pericarditis, perihepatitis, peritonitis, salpingitis, and other extraintestinal diseases. Systemic infection of poultry is characterized in its acute form by septicemia, commonly resulting in sudden death (14,16,52).Previous studies showed that certain subsets of ExPEC strains isolated from different host organisms are highly similar (17,31,44,51), thus increasing the need to study their zoonotic potential. Virulence determinants common to uropathogenic E. coli (UPEC), APEC, neonatal meningitis Escherichia coli (NMEC), or septicemia-associated E. coli (SEPEC), such as the aerobactin iron transport system, the K1 capsule, and type 1 and P fimbriae, have been identified (2,14,19,21,43,47,59). Furthermore, the function of the K1 capsule was similar in virulence to those of APEC and NMEC. The K1...

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PSF is an IbeA-binding protein contributing to meningitic Escherichia coli K1 invasion of human brain microvascular endothelial cells

Cited by 24 publications

References 36 publications

New Role for the ibeA Gene in H2O2 Stress Resistance of Escherichia coli

New Role for the ibeA Gene in H2O2 Stress Resistance of Escherichia coli

Inactivation of ibeA and ibeT Results in Decreased Expression of Type 1 Fimbriae in Extraintestinal Pathogenic Escherichia coli Strain BEN2908

Effects of ibeA Deletion on Virulence and Biofilm Formation of Avian Pathogenic Escherichia coli

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