Pseudozyma vetiver sp. nov., a novel anamorphic ustilaginomycetous yeast species isolated from the phylloplane in Thailand

Abstract

Three strains representing one novel yeast species were isolated from the phylloplanes of the vetiver grasses (DMKU-LV90 and DMKU-LV99(T)) and sugarcane (DMKU-SP260) collected in Thailand by leaf washing followed by a plating technique. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and the sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer region (ITS), the three strains were found to represent a single novel anamorphic ustilaginomycetous yeast species in the genus Pseudozyma. The name Pseudozyma vetiver sp. nov. is proposed for this novel species. The type strain is DMKU-LV99(T) (BCC 61021 = CBS 12824). The novel species showed phylogenetic relationships to the other members of the genus Pseudozyma and to teleomorphic fungal genera, namely Ustilago, Sporisorium and Anomalomyces in Ustilaginaceae, Ustilaginales. The three strains showed identical sequences both in the D1/D2 and ITS regions. The Pseudozyma species closest to the novel species in terms of pairwise sequence similarity in the D1/D2 region was Pseudozyma pruni but with 2.3 % nucleotide substitutions (14 nucleotide substitutions and no gaps out of 606 nt). The novel species and P. pruni differed by 10.9 % nucleotide substitutions (75 nucleotide substitutions and 31 gaps out of 691 nt) in the ITS region. The phylogenetic analysis based on the combined sequences of the ITS region and the D1/D2 region of the LSU rRNA gene showed that the novel species was found to be most closely related to Pseudozyma fusiformata but with 2.9 % nucleotide substitutions in the D1/D2 region and 7.4 % nucleotide substitutions in the ITS region.

Yamadazyma endophytica f.a. sp. nov., an ascomycetous yeast species isolated from leaf tissue

“…D. siamensis, and epiphytic yeast species such as Hannaella siamensis, H. phetchabunensis, H. pagnoccae, Nakazawaea siamensis, Pseudozyma vetiver and Wickerhamomyces siamensis (Chamnanpa et al, 2013;Kaewwichian et al, 2013a, 20142015;Landell et al, 2014). Some species were described based on the strains isolated from the phylloplane and inner tissue of plant leaves such as O. tropicalis, Papiliotrema siamense and Wickerhamomyces siamensis (Surussawadee et al, 2014;Khunnamwong et al, 2014Khunnamwong et al, , 2015a.…”

Multigene phylogeny and taxonomic revision of yeasts and related fungi in the Ustilaginomycotina

“…The genera Acaromyces and Meira contain probably mite-associated species, which are morphologically similar to Pseudozyma species, but phylogenetically belong to different lineages within Exobasidiomycetes ( Boekhout et al., 2003 , Boekhout et al., 2011 , Rush and Aime, 2013 ). The genus Pseudozyma is a polyphyletic anamorphic genus with species occurring in various clusters together with teleomorphic species of Ustilago , Sporisorium and Moesziomyces in the Ustilaginaceae ( Ustilaginales ) ( Begerow et al., 2000 , Begerow et al., 2006 , Begerow et al., 2014 , Stoll et al., 2003 , Stoll et al., 2005 , Liou et al., 2009 , McTaggart et al., 2012a , McTaggart et al., 2012b , Chamnanpa et al., 2013 , Shivas et al., 2013 , Oliveira et al., 2014 ). The genus Farysizyma is an anamorphic genus in the Anthracoideaceae ( Ustilaginales ) described by Inácio et al.…”

A molecular phylogenetic framework for Anthracocystis (Ustilaginales), including five new combinations (inter alia for the asexual Pseudozyma flocculosa), and description of Anthracocystis grodzinskae sp. nov.

“…Sequences were either obtained directly from yeast strains or from environmental samples (as environmental sequences) that likely represent saprobic species. Previously, several phylogenetic studies on yeasts, with limited sampling of teleomorphic species, suggested an affinity of Pseudozyma flocculosa to some species now accommodated in Anthracocystis (e.g., Sampaio 2004;Wang et al 2006;Seo et al 2007;Statzell-Tallman et al 2010;Chamnanpa et al 2013). However, the current study provides the first explicit evidence that several asexual species are nested within the Anthracocystis lineage using broad sampling of teleomorphic species.…”