Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity

Chang, Jo Shu; Chou, Cheng Liang; Lin, Guang Huey; Sheu, Shih Yi; Chen, Wen Ming

doi:10.1016/j.syapm.2004.11.003

Cited by 43 publications

(24 citation statements)

References 20 publications

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“…Other studies on the identiWcation of bacteria contaminating paper mills that were conducted in USA [1,5,35] have also demonstrated that Pseudomonas, Bacillus and Microbacterium were among the paper mill bacterial community. Our results conWrmed the work of Desjardins and Beaulieu [2] suggesting that Pseudoxanthomonas, a bacterial genus associated with various environments such as polluted soil and hot springs [36,37] was a common inhabitant of the warm water of two diVerent Canadian paper machines. In this study, we isolated the same bacterial genera that were previously isolated from headboxes by Desjardins and Beaulieu [2] from the same paper machines.…”

Section: Discussionsupporting

confidence: 87%

Carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a Canadian mill

Kashama

Prince

Simao-Beaunoir

et al. 2009

J Ind Microbiol Biotechnol

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Forty-one bacterial strains isolated from the headbox water of two machines in a Canadian paper mill were associated with the genera Asticcacaulis, Acidovorax, Bacillus, Exiguobacterium, Hydrogenophaga, Pseudomonas, Pseudoxanthomonas, Staphylococcus, Stenotrophomonas based on the sequence of their 16S rRNA genes. The metabolic profile of these strains were determined using Biolog EcoPlate, and the bacteria were divided into four metabolic groups. Metabolic profiles of the bacterial communities colonizing the headbox water of two paper machines was also determined weekly over a 1 year period. The only compound that was not reduced by the bacterial community was 2-hydroxybenzoic acid. Utilization frequency of the other carbon sources in the Biolog EcoPlate ranged from 3 to 100%. The metabolic profiles of the bacterial community did not vary considerably between the two paper machines. However, the metabolic profile varied among the sampling dates.

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Section: Discussionsupporting

confidence: 87%

Carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a Canadian mill

Kashama

Prince

Simao-Beaunoir

et al. 2009

J Ind Microbiol Biotechnol

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“…4B and C and Table 2). In fact, with the exception of D. koreensis, which was not previously related to either alkane degradation or oil-polluted sites, the other species (P. spadix and S. maltophilia) were previously associated with oil degradation and surfactant production (13,72). The alphaproteobacterium genus Erythrobacter, commonly encountered after first fast degradation processes (40,50), was detected as well as part of the population related to alkane degradation (OS-Hx).…”

mentioning

confidence: 94%

Bacterial Communities from Shoreline Environments (Costa da Morte, Northwestern Spain) Affected by the Prestige Oil Spill

Alonso-Gutierrez

Figueras

Albaigés

et al. 2009

Appl Environ Microbiol

129

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The bacterial communities in two different shoreline matrices, rocks and sand, from the Costa da Morte, northwestern Spain, were investigated 12 months after being affected by the Prestige oil spill. Culture-based and culture-independent approaches were used to compare the bacterial diversity present in these environments with that at a nonoiled site. A long-term effect of fuel on the microbial communities in the oiled sand and rock was suggested by the higher proportion of alkane and polyaromatic hydrocarbon (PAH) degraders and the differences in denaturing gradient gel electrophoresis patterns compared with those of the reference site. Members of the classes Alphaproteobacteria and Actinobacteria were the prevailing groups of bacteria detected in both matrices, although the sand bacterial community exhibited higher species richness than the rock bacterial community did. Culture-dependent and -independent approaches suggested that the genus Rhodococcus could play a key role in the in situ degradation of the alkane fraction of the Prestige fuel together with other members of the suborder Corynebacterineae. Moreover, other members of this suborder, such as Mycobacterium spp., together with Sphingomonadaceae bacteria (mainly Lutibacterium anuloederans), were related as well to the degradation of the aromatic fraction of the Prestige fuel. The multiapproach methodology applied in the present study allowed us to assess the complexity of autochthonous microbial communities related to the degradation of heavy fuel from the Prestige and to isolate some of their components for a further physiological study. Since several Corynebacterineae members related to the degradation of alkanes and PAHs were frequently detected in this and other supralittoral environments affected by the Prestige oil spill along the northwestern Spanish coast, the addition of mycolic acids to bioremediation amendments is proposed to favor the presence of these degraders in long-term fuel pollution-affected areas with similar characteristics.

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“…The spectra of substrates utilized is presented in Table 1. Additionally, strain JA40 T oxidizes diverse carbon sources available in Biolog GN and GP, namely a-cyclodextrin, b-cyclodextrin, dextrin, (Thierry et al, 2004); 3, Pseudoxanthomonas mexicana DSM 15133 T (Thierry et al, 2004); 4, Pseudoxanthomonas japonensis JCM 11525 T (Thierry et al, 2004); 5, Pseudoxanthomonas taiwanensis ATCC BAA-404 T (Chen et al, 2002); 6, Pseudoxanthomonas koreensis KCTC 11208 T (Yang et al, 2005); 7, Pseudoxanthomonas daejeonensis KCTC 12207 T (Yang et al, 2005); 8, Pseudoxanthomonas kaohsiungensis LMG 22530 T (Chang et al, 2005 Nitrite is reduced, with the production of nitrous oxide as a main product; nitrate is not reduced.…”

Section: Description Of Pseudoxanthomonas Kalamensis Sp Novmentioning

confidence: 99%

Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll, North Pacific Ocean

Harada

Campbell

2006

International Journal of Systematic and Evolutionary Microbiology

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An aerobic, mesophilic bacterium, strain JA40 T , was isolated from soil contaminated with polycyclic aromatic hydrocarbons and polychlorinated biphenyls collected from Johnston Atoll in the North Pacific Ocean. The strain formed yellow-pigmented colonies on heterotrophic media. The cells were Gram-negative, non-motile, non-sporulating rods. The strain reduced nitrite to nitrous oxide, the DNA G+C content was 64 mol% and the dominant fatty acids were 15 : 0 iso, 17 : 1 iso cis7 and 11 : 0 iso 3-OH. DNA sequencing of 1457 nt of the 16S rRNA gene established that JA40 T belongs in the genus Pseudoxanthomonas within the Xanthomonadaceae branch of the Gammaproteobacteria. Strain JA40 T can be differentiated from other mesophilic species in the genus on the basis of its physiological and biochemical characteristics and distinctive fatty acid profile. Thus strain JA40 T (=ATCC BAA-1031 T =CIP 108476 T ) is the type strain of a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas kalamensis sp. nov. is proposed. The genera Stenotrophomonas, Xanthomonas and Pseudoxanthomonas partly comprise the family Xanthomonadaceae in the Gammaproteobacteria (Finkmann et al., 2000;Yang et al., 2005). These genera share phenotypic traits (e.g. colony shape and colour, cell morphology, Gram reaction), the presence of branched-chain fatty acids and the presence of ubiquinones with eight isoprenoid units (Q-8). Members of the genus Pseudoxanthomonas, however, are differentiated from members of the genera Xanthomonas and Stenotrophomonas by their ability to reduce nitrite but not nitrate to N 2 O and by the absence of the fatty acid C 13 : 0 iso 3-OH (Thierry et al., 2004). Here we describe the isolation of strain JA40 T and its characterization as a novel species of the genus Pseudoxanthomonas. Johnston Atoll is located in the NorthStrain JA40 T was isolated from soil contaminated with polycyclic aromatic hydrocarbons and polychlorinated biphenyls from Johnston Atoll. The strain was originally cultivated from serial dilutions of soil spread-plated on marine agar 2216 (MA; Difco). Colonies that arose after 3-5 days incubation at 30 uC were streaked for isolation on MA. Strain purity was checked (through repeated transfers) by assessing the consistency of the colony characteristics, the cell morphology and the Gram reaction. Strain JA40 T was maintained on MA or in marine broth 2216 (MB; Difco). Stock cultures were stored at 280 u C in MB with 50 % (v/v) glycerol.Unless noted, all characterizations of strain JA40 T were performed on cells grown for 5 days at 30 uC on MA. Motility was checked by means of the hanging drop method using light microscopy under a 6100 objective with oil immersion. The morphology of negatively stained cells (1 % uranyl acetate) was observed in an LEO 912 energy-filtering transmission electron microscope. Images were recorded as digital files with a Pro-scan frame transfer CCD camera. Cells prepared for transmission electron microscopy were grown for 4 days on tryptic soy agar (TSA; D...

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Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity

Cited by 43 publications

References 20 publications

Carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a Canadian mill

Carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a Canadian mill

Bacterial Communities from Shoreline Environments (Costa da Morte, Northwestern Spain) Affected by the Prestige Oil Spill

Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll, North Pacific Ocean

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