Pseudoxanthobacter soli gen. nov., sp. nov., a nitrogen-fixing alphaproteobacterium isolated from soil

Arun, A. B.; Schümann, Peter; Chu, Horng-I; Tan, Chen-Chung; Chen, Wen-Ming; Lai, Wei-An; Kämpfer, Peter; Shen, Fo-Ting; Rekha, P. D.; Hung, Mei-Hua; Chou, Jui-Hsing; Young, Chiu-Chung

doi:10.1099/ijs.0.65206-0

Cited by 19 publications

(9 citation statements)

References 15 publications

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“…Taxonomic identification of the strains was carried out by 16S rRNA gene sequencing as described elsewhere [14] . Briefly, the genomic DNA was extracted from the 48 h grown bacterial culture using genomic DNA extraction kit (Mobio Inc).…”

Section: Taxonomic Identification Of the Selected Isolatesmentioning

confidence: 99%

Prospecting exopolysaccharides produced by selected bacteria associated with marine organisms for biotechnological applications

Priyanka

Arun

Young³

et al. 2014

Chin J Polym Sci

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In this study, bacteria associated with marine organisms were screened for the production of exopolysaccharides (EPSs) on MY media containing sea salts (2.5%10%). Three selected isolates were identified as Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26 using 16S rRNA gene sequencing. Optimization of the growth and EPS production kinetics in relation to incubation time were assessed. The purified EPS yield was 590, 650 and 540 mg·L 1 culture media respectively in Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26. Biochemical and FTIR analyses revealed the presence of biologically important functional groups in the EPS produced by all the three isolates. The EPS produced by Nitratireductor sp. PRIM-24 and Alteromonas sp. PRIM-21 showed 2.0% sulfate content. These bacterial EPS also showed antioxidant and emulsifying activities and the EPS produced by Enterobacter sp.PRIM-26 showed significantly higher antioxidant activities in terms of superoxide (IC 50 0.33 mg·mL 1 ) and DPPH (IC 50 0.44 mg·mL 1 ) radical scavenging. It also showed higher emulsifying activities against selected hydrophobic substrates with EI 24 values above 60%. From the results of the study, it can be concluded that the isolated bacteria produce EPS that can be investigated in detail for biotechnological applications.

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Section: Taxonomic Identification Of the Selected Isolatesmentioning

confidence: 99%

Prospecting exopolysaccharides produced by selected bacteria associated with marine organisms for biotechnological applications

Priyanka

Arun

Young³

et al. 2014

Chin J Polym Sci

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“…The genus Pseudoxanthobacter , in the family Xanthobacteraceae , was first proposed by Arun et al (2008) to accommodate some Gram-stain negative, aerobic, short, rod-shaped and non-spore-forming micro-organisms. At the time of writing, there is only one species in the genus with a validly published name, Pseudoxanthobacter soli CC4 T (Arun et al , 2008). During the isolation of potential dichlorodiphenyltrichloroethane (DDT)-degrading micro-organisms from a soil sample that had been contaminated with DDT for many years in Liyang, PR China, a bacterial strain, designated DDT-3 T , was isolated.…”

mentioning

confidence: 99%

“…Minor amounts of C 18 : 0 (4.0 %) and C 18 : 0 3-OH (1.8 %) were also found. The presence of the major fatty acids C 19 : 0 cyclo ω8 c , C 16 : 0 and C 18 : 1 ω7 c affiliated strain DDT-3 T with other members of the genus Pseudoxanthobacter (Arun et al , 2008). However, differences in fatty acid profiles were also found between DDT-3 T and CC4 T (Table S1).…”

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confidence: 99%

Pseudoxanthobacter liyangensis sp. nov., isolated from dichlorodiphenyltrichloroethane-contaminated soil

Liu

Chen

Meng

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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An aerobic, Gram-stain negative, pale, rod-shaped, nitrogen-fixing bacterial strain, DDT-3 T , was isolated from dichlorodiphenyltrichloroethane-contaminated soil in Liyang, PR China. Strain DDT-3 T grew at temperatures ranging from 20 to 40 6C (optimum 30-37 6C) and a pH of between 5.0 and 10.0 (optimum pH 7.0-8.0). The G+C content of the total DNA was 70.1 mol%. The 16S rRNA gene sequence of strain DDT-3 T showed the highest similarity to that of Pseudoxanthobacter soli CC4 T (99.6 %), followed by Kaistia dalseonensis B6-8 T (93.3 %), Kaistia soli 5YN9-8 T (93.0 %) and Amorphus orientalis YIM D10 T (93.0 %). Strain DDT-3 T showed less than 92.6 % similarity with other species of the family Xanthobacteraceae. The major cellular fatty acids of strain DDT-3 T were C 19 : 0 cyclo v8c (42.6 %), C 16 : 0 (33.2 %) and C 18 : 1 v7c (10.0 %). The only respiratory quinone was ubiquinone Q-10. The characteristic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The major polar lipids were glycolipid, lipid, phosphatidylcholine, aminolipid, phosphatidylmethylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The polyamine profile consisted of major amounts of putrescine (92.9 %) and minor amounts of spermidine (5.0 %) and spermine (2.1 %). These chemotaxonomic data support the affiliation of strain DDT-3 T with the genus Pseudoxanthobacter. The DNA-DNA hybridization value between strain DDT-3 T and strain CC4 T was 47.8 % (reciprocal 44.3 %). DNA-DNA hybridization data as well as the biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain DDT-3 T and strain CC4 T . Strain DDT-3 T , therefore, represents a novel species of the genus Pseudoxanthobacter, for which the name Pseudoxanthobacter liyangensis sp. nov. is proposed. The type strain is DDT-3 T (5KACC 16601 T 5CCTCC AB 2013167 T ).The genus Pseudoxanthobacter, in the family Xanthobacteraceae, was first proposed by Arun et al. (2008) to accommodate some Gram-stain negative, aerobic, short, rod-shaped and non-spore-forming micro-organisms. At the time of writing, there is only one species in the genus with a validly published name, Pseudoxanthobacter soli CC4 T (Arun et al., 2008). During the isolation of potential dichlorodiphenyltrichloroethane (DDT)-degrading microorganisms from a soil sample that had been contaminated with DDT for many years in Liyang, PR China, a bacterial strain, designated DDT-3 T , was isolated. Strain DDT-3 T was found to be able to adsorb DDT but not to degrade it, which was confirmed by ultra-violet scanning and gaschromatographic analysis. The strain was then characterized using a polyphasic approach, including the analysis of chemotaxonomic and phenotypic properties; phylogenetic analysis was based on 16S rRNA gene sequence similarities and genomic relatedness. On the basis of the results obtained in this study, we propose that strain DDT-3 T should be placed within the genus Pseudoxanthobacter as the type strain of a novel species, with the suggested ...

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“…The occurrence of ubiquinone Q-10 as the predominant quinone is in accordance with earlier reports on members of the family Xanthobacteraceae (summarized by Hwang & Cho, 2008). Ubiquinone Q-9 has been detected in species of the genus Xanthobacter (Wiegel, 2005) and the phylogenetically more distant Pseudoxanthobacter soli (Arun et al , 2008), and Q-11 has been found in the distantly related genus Devosia (Yoon et al , 2007).…”

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confidence: 99%

Azorhizobium oxalatiphilum sp. nov., and emended description of the genus Azorhizobium

Lang

Schümann

Adler

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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Pseudoxanthobacter soli gen. nov., sp. nov., a nitrogen-fixing alphaproteobacterium isolated from soil

Cited by 19 publications

References 15 publications

Prospecting exopolysaccharides produced by selected bacteria associated with marine organisms for biotechnological applications

Prospecting exopolysaccharides produced by selected bacteria associated with marine organisms for biotechnological applications

Pseudoxanthobacter liyangensis sp. nov., isolated from dichlorodiphenyltrichloroethane-contaminated soil

Azorhizobium oxalatiphilum sp. nov., and emended description of the genus Azorhizobium

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