Pseudotyping of Moloney leukemia virus-based retroviral vectors with simian immunodeficiency virus envelope leads to targeted infection of human CD4+ lymphoid cells

Abstract

In view of our recent findings that a truncated form of the CD4 and sera from SIV-infected macaques. We also envelope (Env) glycoprotein of human immunodeficiency observed pseudotype-mediated gene transfer of a green virus type 1 (HIV-1) was efficiently incorporated into fluorescent protein marker into the CD4 + CEMX174 and MoMLV particles, we studied the generation of Moloney C8166 lymphoid cell lines. More importantly, primary murine leukemia virus (MoMLV)/simian immunodeficiency human lymphocytes were also successfully transduced ex virus (SIV) pseudotypes. Unlike HIV-1, both the wild-type vivo by MoMLV/SIV pseudotypes, albeit at lower efficiency, SIV Env and a truncated form, which lacks most of the and gene transfer was specifically restricted to the CD4 + cytoplasmic domain of the transmembrane glycoprotein, subset. These findings demonstrate that MoMLV/SIV were incorporated into MoMLV particles and generated pseudotypes can be used to transduce cells which are susinfectious retroviral vectors which could transduce CD4 + ceptible to SIV infection, and thus might be advantageously sMAGI macaque cells. The infection depended on target employed in animal models for direct in vivo delivery of cell CD4 expression, and was neutralized by both soluble gene therapy-based approaches.

Selective transduction of protease-rich tumors by matrix-metalloproteinase-targeted retroviral vectors

“…26 This limitation provided the impetus for a number of groups to engineer novel lentiviral vectors from different species of lentivirus, all of which are capable of transducing quiescent cells. [27][28][29] In light of these developments, it is worth noting that MLV envelope glycoproteins can be pseudotyped into lentiviral vectors and the protease targeting strategies described in this study should therefore be readily transferable from C-type retroviral vectors to lentiviral vectors. Experiments to test this assumption are currently underway.…”

Pseudotyping of Porcine Endogenous Retrovirus by Xenotropic Murine Leukemia Virus in a Pig Islet Xenotransplantation Model

“…Thus, it is reasonable to consider the possibility that pseudotyping by human endogenous or exogenous retrovirus, with a consequent expansion of PERV tropism, could be observed in xenotransplanted human patients. For example, MuLV retroviral particles can be pseudotyped by the incorporation of full length, naturally occurring simian immunodeficiency virus env proteins (46). On the other hand, only a truncated version of HIV env is capable of pseudotyping MuLV virions, though the potential of this occurring in a natural state is unknown (47).…”

Differential effects of angiostatin, endostatin and interferon-α1 gene transfer on in vivo growth of human breast cancer cells

“…Infectious particles carrying the angiostatin, the endostatin, and the control LE retroviral genomes were generated by a transient three-plasmid vector-packaging system, as previously described. 41 Briefly, 293T cells were seeded in 100-mm-diameter Petri dishes at 5 × 10 6 cells/dish, and transfected overnight according to a calcium-phosphate protocol using 12 g of a plasmid DNA encoding the different retroviral vector genomes, 6 g of a Mo-MLV gagpol expression construct, and 0.3 g of a VSV-G expression plasmid, which encodes the envelope used by the vectors in this study. Thirty-six hours after transfection, culture medium was replaced with fresh DMEM without FCS.…”