Pseudonocardia acaciae sp. nov., isolated from roots of Acacia auriculiformis A. Cunn. ex Benth.

Abstract

Pseudonocardia acaciae sp. nov., isolated from roots of Acacia auriculiformis A. Cunn. ex Benth. Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan A novel Gram-positive-staining actinomycete designated strain GMKU095 T was isolated from surface-sterilized roots of Acacia auriculiformis A. Cunn. ex Benth. (earpod wattle). The organism produced branching mycelium. The spores were non-motile and had a spiny surface. Growth of strain GMKU095 T occurred at 18-42 6C, pH 5.0-8.0 and at NaCl concentrations up to 5 %. Whole-cell hydrolysates contained arabinose and galactose as major characteristic sugars. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The glycan moiety of the murein contained acetyl residues. The menaquinone was MK-8(H 4 ); mycolic acids were not detected. The G+C content of the DNA was 71.6 mol%. iso-C 16 : 0 was detected as the major cellular fatty acid. Comparative studies of 16S rRNA gene sequences indicated that the strain was phylogenetically related to members of the genus Pseudonocardia. The most closely related type strain is Pseudonocardia spinosispora IMSNU 50581 T , which is 96.2 % similar in 16S rRNA gene sequence. On the basis of the genotypic and phenotypic properties presented, a novel species of the genus Pseudonocardia is proposed, Pseudonocardia acaciae sp. nov. The type strain is strain GMKU095 T

Pseudonocardia sichuanensis sp. nov., a novel endophytic actinomycete isolated from the root of Jatropha curcas L.

“…Its description was later emended by McVeigh et al (1994), Warwick et al (1994), Reichert et al (1998), Huang et al (2002) and Park et al (2008). At the time of writing, there are 36 recognized species belonging to the genus Pseudonocardia, including the recently described organisms P. oroxyli (Gu et al 2006), P. endophytica (Chen et al 2009), P. acaciae (Duangmal et al 2009) and P. tropica (Qin et al 2009b) that were all isolated from the endophytic environments of surface sterilized plants. During a study of the diversity and function of endophytic actinobacteria from oil-seed plant Jatropha curcas L., an actinomycete strain, designated KLBMP 1115 T was isolated that exhibited the typical morphological characteristics of the genus Pseudonocardia.…”

Pseudonocardia nematodicida sp. nov., isolated from mangrove sediment in Hainan, China

“…net/pseudonocardia.html). Bacterial species of this genus have been isolated from various environments, such as soil (Henssen et al 1983;Lee et al 2002;Park et al 2008;Qin et al 2008;Ara et al 2011), plant roots (Duangmal et al 2009;Kaewkla and Franco 2011;Zhao et al 2011), plant stem (Qin et al 2010), coastal sediment (Liu et al 2006), deep-sea sediment (Tian et al 2013), industrial sludge (Mahendra and AlvarezCohen 2005) and a gold mine cave (Lee et al 2001). Mangrove ecosystems, as highly productive wetlands distributed along estuaries, provide refuge, growing, breeding and feeding zones for many marine organisms (Holguin et al 2001).…”

Pseudonocardia yuanmoensis sp. nov., a novel actinobacterium isolated from soil in Yunnan, south-west China

“…The DNA G?C contents are from 68 to 79 mol%. In recent years, members of the genus Pseudonocardia have been reported widely and have been isolated mostly from plant samples (including stems, leaves, root nodules, tree-bark composts and traditional medicinal plants) (Gu et al 2006;Chen et al 2009;Duangmal et al 2009;Franco 2010, 2011;Qin et al 2010Qin et al , 2011Zhao et al 2011b), some also have been isolated from soil samples (including polluted soil samples by industrial chemical) (Qin et al 2008;Mahendra and Cohen 2005), coastal sediment ) and so on.…”