Pseudomonas versuta sp. nov., isolated from Antarctic soil

See-Too, Wah-Seng; Salazar, Sergio Miguel; Ee, Robson; Convey, Peter; Chan, Kok‐Gan; Peix, Álvaro

doi:10.1016/j.syapm.2017.03.002

Cited by 23 publications

(13 citation statements)

References 49 publications

(49 reference statements)

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“…; See‐Too et al . ). Pseudomonas veronii has been isolated from natural mineral water (Elomari et al .…”

Section: Discussionmentioning

confidence: 97%

“…By now, there have been no reports of the abundance of these species on meat or contribution to spoilage. Pseudomonas versuta and P. meridiana have been isolated from antarctic soil, although they are described as psychrophilic, the reported observed growth occurred at 4-30°C (Reddy et al 2004;See-Too et al 2017). Pseudomonas veronii has been isolated from natural mineral water (Elomari et al 1996) and P. simiae from clinical samples of primates (Vela et al 2006).…”

Section: Spoilage-associated Microbiota On Map Minced Beefmentioning

confidence: 99%

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Diversity and anaerobic growth ofPseudomonasspp. isolated from modified atmosphere packaged minced beef

et al. 2019

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Aims This study aimed to monitor development of spoilage‐associated microbiota on high‐oxygen modified atmosphere packaged (MAP) minced beef, assess diversity of Pseudomonas sp. therein employing a polyphasic approach and probe their ability to grow anaerobically in the presence of carbon dioxide. Methods and Results Headspace atmosphere and total viable count of MAP minced beef were monitored, and spoilage‐associated microbiota was identified using matrix‐assisted laser desorption/ionization time of flight mass spectrometry (MALDI‐TOF MS). Pseudomonas spp. represented a major part of the spoilage‐associated microbiota throughout the spoilage process and were characterized with a polyphasic approach including MALDI‐TOF, randomly amplified polymorphic DNA biotyping, 16S rDNA and rpoD sequence analysis, and carA multiplex polymerase chain reaction. Pseudomonas isolates displayed high diversity and varying assertiveness under conditions employed in MAP minced beef with P. fragi, P. lundensis and P. weihenstephanensis as dominant species. Conclusions The polyphasic approach enabled high‐throughput characterization of Pseudomonas sp. Their adapted capability to grow anaerobically and resistance to high levels of CO2 is suggested to be a general feature within the genus, which is hitherto underexplored. Significance and Impact of the Study This study shows that diverse Pseudomonas generally regarded as strict aerobes and CO2‐sensitive appear well adapted to grow under MAP conditions, leading to high cell counts in minced beef and ultimately contribute to spoilage of the product.

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“…; See‐Too et al . ). Pseudomonas veronii has been isolated from natural mineral water (Elomari et al .…”

Section: Discussionmentioning

confidence: 97%

Section: Spoilage-associated Microbiota On Map Minced Beefmentioning

confidence: 99%

Diversity and anaerobic growth ofPseudomonasspp. isolated from modified atmosphere packaged minced beef

et al. 2019

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“…paraversuta beside [ Pseudomonas ] versuta ), referring to the close relationship to the recently described species P. versuta [20, 46].…”

Section: Description Of Pseudomonas Paraversuta Sp Novmentioning

confidence: 99%

“…fragi subgroup has expanded and now includes eleven acknowledged members. The more recently described members are Pseudomonas psychrophila detected in a food storage cold room in Japan in 2001 [15], whose isolation originally dates back to 1978 [16], Pseudomonas deceptionensis from marine sediment of the Antarctic area in 2011 [17], Pseudomonas endophytica from stem tissue of Solanum tuberosum in Spain in 2015 [18], Pseudomonas helleri and Pseudomonas weihenstephanensis isolated from raw cow’s milk in Germany in 2016 [19], Pseudomonas versuta from Antarctic soil in 2017 [20], Pseudomonas saxonica from raw milk in Germany in 2020 [21] and Pseudomonas bubulae from beef in Germany in 2020 [22]. In this work, we propose a novel member of the P.…”

Section: Full-textmentioning

confidence: 99%

Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef

Lick

Wibberg

Winkler

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A polyphasic approach was applied to investigate the diversity of microbiota that evolved during cold storage beef ripening. Isolate V4/DAB/S4/2aT with a unique BOX-rep-PCR fingerprint profile revealed more than 99 % nucleotide identities upon pairwise comparisons of 16S rDNA sequences from the type strains Pseudomonas versuta DSM 101070T, Pseudomonas saxonica DSM 108989T, Pseudomonas deceptionensis DSM 26521T and Pseudomonas weihenstephanensis DSM 29166T, placing it within the Pseudomonas fragi / lundensis branch of the genus Pseudomonas . Additional rpoB based comparison revealed P. versuta DSM 101070T as the nearest relative, with 98.5 % nucleotide identity. Calculation of ANIb values of the V4/DAB/S4/2aT draft genome identified P. versuta DSM 101070T with 90.1 %, P. deceptionensis DSM 26521T with 85.1 %, P. fragi DSM 3456T with 84.4 %, Pseudomonas psychrophila DSM 17535T and Pseudomonas bubulae DSM 107389T with 84.2 % similarities each. Pairwise genome-to-genome distance calculations [digital DNA–DNA hybridization (dDDH)] resulted in values of 47.1, 35.1, 34.8, 34.2 and 34.1 %, respectively. A second isolate was detected years later in ground beef and showed ANIb values of 99.3 % and dDDH of 96.1 % relatedness to V4/DAB/S4/2aT. The DNA G+C content was 58.6 mol% for both isolates. The predominant cellular fatty acids of V4/DAB/S4/2aT were C16 : 0, C18 : 1ω7c, C17 : 0 cyclo and a summed feature containing C16 : 1ω7c and/or C15 : 0 iso 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, the major respiratory quinone was Q9, with a small portion of Q8. The combined data on genotypic and phenotypic features support the proposal of a novel species, for which the name Pseudomonas paraversuta sp. nov. is proposed. The type strain is V4/DAB/S4/2aT (=DSM 111361T=LMG 31844T) and a second isolate is UBT376 (=DSM 111360=LMG 31845).

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“…From these first reports, several Antarctic isolates have been described, some of them phylogenetically related to psychrotolerant strains described in temperate environments, while others have been defined as novel species. Among them, we can include Pseudomonas antarctica, Pseudomonas meridiana and Pseudomonas proteolytica, characterized from mats of cyanobacteria included in continental water bodies [9], Pseudomonas extremaustralis obtained from temporary freshwater ponds [10], Pseudomonas deceptionensis characterized from marine sediments of Deception Island [11], Pseudomonas pelagia isolated from a culture of an Antarctic green microalga [12], Pseudomonas gregormendelii isolated from volcanic rock on Ulu Peninsula of James Ross Island [13], and Pseudomonas guineae [14], Pseudomonas prosekii [15] and Pseudomonas versuta [16], identified from soil samples from South Shetland, James Ross and Lagoon Islands, respectively. The present study aimed to characterize the strain KG01 T taxonomically through a polyphasic approach and to formally propose it as a novel Antarctic species of the genus Pseudomonas.…”

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confidence: 99%

Pseudomonas fildesensis sp. nov., a psychrotolerant bacterium isolated from Antarctic soil of King George Island, South Shetland Islands

Pavlov

Lira

Martínez

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The strain KG01T was isolated from a soil sample from King George Island, Antarctica. Cells of KG01T are rod-shaped and motile by means of multiple polar flagella. The absence of arginine dihydrolase activity could be a key feature to readily distinguish KG01T from its closest phylogenetic relative species. The main fatty acids of the strain include summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH), C16 : 0 and C18 : 1 ω7c. Phylogenetic analysis based on the 16S rRNA gene sequence and on a multilocus sequence analysis (MLSA) using housekeeping genes (16S rRNA, rpoB, rpoD, gyrB) were carried out. These analyses allowed us to include the strain within the Pseudomonas fluorescens group, presenting the highest similarity of multilocus sequence with Pseudomonas veronii LMG 17761T (96.67 %). The genome of KG01T was sequenced and in silico compared with genomes of the most closely related species of the P. fluore scens group. The average nucleotide identity (ANIb) and average amino acid identity (AAI) values of the species phylogenetically closest to KG01T were less than 95–96 %, threshold currently accepted to define strain as belonging to a bacterial species, the highest scores being those to Pseudomonas veronii LMG 17761T (87.98 %) and Pseudomonas marginalis ICMP 3553T (91.90 %). Therefore, the phenotypic and genotypic analyses results, allow us to propose that KG01T represents a member of a novel species of the genus Pseudomonas , for which the name Pseudomonas fildesensis is proposed, and KG01T (=CECT 9084T;=DSM 102036T) is established as the type strain .

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Pseudomonas versuta sp. nov., isolated from Antarctic soil

Cited by 23 publications

References 49 publications

Diversity and anaerobic growth ofPseudomonasspp. isolated from modified atmosphere packaged minced beef

Diversity and anaerobic growth ofPseudomonasspp. isolated from modified atmosphere packaged minced beef

Pseudomonas paraversuta sp. nov. isolated from refrigerated dry-aged beef

Pseudomonas fildesensis sp. nov., a psychrotolerant bacterium isolated from Antarctic soil of King George Island, South Shetland Islands

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