Pseudomonas umsongensis GO16 as a platform for the in vivo synthesis of short and medium chain length polyhydroxyalkanoate blends

Cerrone, Federico; Zhou, Binbin; Mouren, Agathe; Avérous, Luc; Conroy, Stephen; Simpson, Jeremy C.; O’Connor, Kevin E.; Narancic, Tanja

doi:10.1016/j.biortech.2023.129668

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…5 mg sample was mixed with 200 mg of powdered KBr. After pulverization, mixture (sample and KBr) placed in to pellet forming machine and resulting pellet was taken for IR analysis ( Cerrone et al, 2023 ). To analyze the chemical shift of the PHA, we utilized 1H Nuclear Magnetic Resonance (NMR) by dissolving a 5 mg sample in 600 μL of CDCl 3 , employing a (BRUKER Proton NMR 500 MHz), as described by Kacanski et al (2023) .…”

Section: Methodsmentioning

confidence: 99%

Acid hydrolysis of Solanum tuberosum periderm for accumulation of polyhydroxyalkanoates in Pseudomonas putida MTCC 2475

Kag,

Kumar,

Kataria

2024

Front. Bioeng. Biotechnol.

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Polyhydroxyalkanoates are a class of biodegradable, biocompatible polymers composed of polyesters of R-hydroxyalkanoic acids and deposited intracellularly by a variety of microorganisms which have potential to serve as alternative to commercial plastic. Bioplastics are gaining attention due to sustainability, biodegradability, biocompatibility, and lower carbon footprint. Nevertheless, the commercialization of PHA is predominantly hindered by the elevated production expenses arising primarily from the use of a pure sugar substrate. Our study has established a feasible method for bioplastic formation applying Pseudomonas putida MTCC 2475 and Solanum tuberosum periderm as a carbon source. To optimize the sugar yield response surface methodology was used, which released 69.34% ± 0.25% reducing sugar. PHA production experiments were performed in hydrolysate containing media as well as commercial sugar containing mineral salt media. After 48 h of fermentation of using this sugar, a biomass concentration of 2.19 gL−1, with a PHA production of 0.60 gL−1 (28.71% ± 0.55%) was obtained which was comparatively similar with synthetic media (2.56 gL−1 cell dry weight and 29.97% ± 0.45% PHA). Furthermore, the monomers of PHA produced by hydrolysate were characterized using Gas chromatography-mass spectrometry, Fourier transform infrared spectroscopy, differential scanning calorimetry, and nuclear magnetic resonance. This investigation has identified three distinct monomers of medium-chain PHAs, namely, methyl 3-Hydroxydodecanoate, 3-Hydroxytetradecanoate, and Hexadecanoic acid 3-Hydroxy methyl esters. Hence this study concludes a sustainable production of bioplastics from S. tuberosum periderm waste.

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Section: Methodsmentioning

confidence: 99%

Acid hydrolysis of Solanum tuberosum periderm for accumulation of polyhydroxyalkanoates in Pseudomonas putida MTCC 2475

Kag,

Kumar,

Kataria

2024

Front. Bioeng. Biotechnol.

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“…The observed minor initial breakdown could be attributed to the presence of impurities. 55 Significantly, the primary decomposition temperature for mcl-PHA is well above T m , indicating a wide processing window for the polymer, in contrast to PHB, where the processing range is limited to 200−230 °C.…”

Section: Acs Sustainable Chemistry and Engineeringmentioning

confidence: 96%

Tailoring Pseudomonas putida feedstocks for enhanced medium-chain-length polyhydroxyalkanoate production and biomedical nanoemulsion applications

Nur-A-Tomal,

Attenborough,

Mazrad

et al. 2024

ACS Sustainable Chem. Eng.

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“…PHO was kindly bioproduced and supplied by the University of Dublin in the frame of MIX-UP Project (Horizon 2020 research and innovation program under grant agreement No 870294). PHO was bioproduced according to the protocol given by Cerrone et al (Cerrone et al, 2023).…”

Section: Chemicals and Materialsmentioning

confidence: 99%

“…These monomers can then be converted by genetically modified bacteria to produce PHAs (Tiso et al, 2021). Depending on the substrate structure and the enzymes involved, a wide range of bacterial polymers can be obtained, differentiated by their number of carbons per repeat unit (Cerrone et al, 2023). Among them, medium side-chain length (mcl) PHAs have repeat units of 6 to 12 carbons, such as poly(3-hydroxy-octanoate) (PHO).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and evaluation of novel sustainable aliphatic plasticizers into different polymer systems.

Mouren,

Pollet,

Avérous

2023

Preprint

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Plasticizers are essential to the polymer industry to (i) facilitate the processing and (ii) modify the mechanical and thermal performance of polymer materials. The need to reduce the environmental impact and toxicity of plasticizers means that viable alternatives to phthalates must be found. This has become a major current challenge. Bacterial polymers such as polyhydroxyalkanoates (PHA) like poly(hydroxy-octanoate) (PHO) appear to be promising precursors for meeting these expectations. Bacterially synthesized from sustainable resources such as plastic waste or biomass, PHOs possess ester functions and alkyl side chains that are relevant for plasticizing some rigid polymers such as PVC or thermoplastic polyurethanes (TPU). In this preliminary and conceptual study, oligomers of PHO with terminal hydroxyl functions (PHO-diol) were successfully synthesized by transesterification from PHO. The addition of alkyl chain ends was achieved by reaction with hexyl mono-isocyanate, to produce PHO-hexyl. These 2 compounds were tested for their plasticizing properties. Rigid PVC and TPU matrices were selected to evaluate the different additives (PHO-diol, PHO-hexyl and two conventional reference phthalates) at different concentrations. Polymer glass transition temperatures (Tg) were significantly reduced with all plasticizers. Plasticizing effects similar to those of phthalates were obtained with PHO-hexyl, both on the thermal and mechanical properties, compared to PHO-diol wich shows less plasticizing effects. The processability of a very rigid and brittle bio-based TPU was greatly improved with the addition of just 15 % of PHO-hexyl as plasticizer into the TPU matrix, as well as with the commercial phthalates. It is opening up a wide range of applications for this previously hardly exploitable sustainable betulin-based TPU. PHO-hexyl is thus emerging as a very promising example of a sustainable plasticizer and a potentially credible alternative to phthalates. This new plasticizer makes it possible to obtain high-performance materials that are more respectful of the environment.

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Pseudomonas umsongensis GO16 as a platform for the in vivo synthesis of short and medium chain length polyhydroxyalkanoate blends

Cited by 7 publications

References 42 publications

Acid hydrolysis of Solanum tuberosum periderm for accumulation of polyhydroxyalkanoates in Pseudomonas putida MTCC 2475

Acid hydrolysis of Solanum tuberosum periderm for accumulation of polyhydroxyalkanoates in Pseudomonas putida MTCC 2475

Tailoring Pseudomonas putida feedstocks for enhanced medium-chain-length polyhydroxyalkanoate production and biomedical nanoemulsion applications

Synthesis and evaluation of novel sustainable aliphatic plasticizers into different polymer systems.

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