Pseudomonas syzygii, sp. nov., the Cause of Sumatra Disease of Cloves

Analysis of the Phylogenetic Relationships of Strains of Burkholderia solanacearum, Pseudomonas syzygii, and the Blood Disease Bacterium of Banana Based on 16S rRNA Gene Sequences

“…Strains of the same species are expected to exhibit DNA reassociation values of more than 70% (30), whereas the levels of DNA-DNA hybridization between the members of different biovars of B. solanacearum have commonly been substantially less than 70% (23,25). However, Roberts et al (25) and Palleroni and Doudoroff (23) found that the techniques used to measure levels of DNA-DNA hybridization of B. solanacearum strains resulted in inherent variability (25) or produced inconsistent results (23) and may therefore only be useful as a guide to the relatedness of strains. It is evident from restriction fragment length polymorphism analysis data, 16s rRNA gene sequence data, and phenotypic differences that the two divisions of B. solanacearum may represent subspecies, as suggested by Li et al (18), or separate species.…”

“…Two close relatives of B. sofanacearum, the BDB, which causes blood disease of bananas in Indonesia (6, 32), and P. syzygii, which causes Sumatra disease of cloves in Indonesia, were included in this study to cIarify their taxonomic status. P. syzygii and the BDB are found only in Indonesia (5), have similar fatty acid profiles (25,31), and are serologically related (1,25) to B. solanacearum. DNA homology studies have shown that P. syzygii is closely related to B. solanacearum (25), and partial sequencing of the 16s rRNA gene has confirmed the close relationship of B. solanacearum, the BDB, and P. syzygii (28).…”

“…P. syzygii and the BDB are found only in Indonesia (5), have similar fatty acid profiles (25,31), and are serologically related (1,25) to B. solanacearum. DNA homology studies have shown that P. syzygii is closely related to B. solanacearum (25), and partial sequencing of the 16s rRNA gene has confirmed the close relationship of B. solanacearum, the BDB, and P. syzygii (28). Partial sequencing of the 16s rRNA gene of the BDB by Seal et al (28) showed that the first 292 bases are identical to the bases found in some B. solanacearum isolates, including isolate R142 (which was also included in this study), and that P. syzygiii has the same sequence as the BDB and R142 but its sequence is one base shorter.…”

“…Pseudomonas syzygii, the causal agent of Sumatra disease of cloves (Syzygium aromaticum), is a close relative of B. solanacearum, as determined by phenotypic data and DNA-DNA hybridization data (25). The blood disease bacterium (BDB), the causal agent of blood disease of bananas, affects certain members of the Musaceae in Indonesia (1,6).…”

Transformação genética de laranja doce (Citrus sinensis L. Osbeck) com o gene cecropin MB39 e avaliação de plantas transgênicas inoculadas com Xylella fastidiosa Wells et al.

“…(ROBERTS et al, 1990;VANEECHOUTTE et al, 2004) e Clavibacter xyli (DAVIS et al, 1984). A X. fastidiosa é responsável pela causa de várias doenças em inúmeras espécies de plantas e também coloniza outras plantas sem mostrar sintomas aparentes (PURCELL; HOPKINS, 1996).…”