Pseudomonas syringae pv. actinidiae strains isolated from past and current epidemics to Actinidia spp. reveal a diverse population structure of the pathogen

Ferrante, Patrizia; Takikawa, Yuichi; Scortichini, M.

doi:10.1007/s10658-015-0643-6

Cited by 16 publications

(9 citation statements)

References 38 publications

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“…Only strain WA29 did not possess these genes. The lack of one or two genes can be explained by their absence or a mutation in the genes that did not allow for their amplification (Ferrante et al 2015). In contrast to our results, in the studies of Abbasi et al (2013), Gilbert et al (2009), and Abdellatif et al (2017) all Pss strains had the syrB gene.…”

Section: Resultscontrasting

confidence: 99%

Bacterial etiology of necrotic spots on leaves and shoots of grapevine (Vitis vinifera L.) in Poland

et al. 2020

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Dark brown, spotted necrotic areas were observed on the leaves and young shoots of grapevine (Vitis vinifera) plants of cvs. Fanny and Muscat Bleu growing on plantations in Central Poland. From 35 samples of symptomatic leaf and young shoot tissues, 70 bacterial isolates were obtained, of which 16 induced HR on tobacco leaves, but only two showed pectolytic activity on potato tubers and CVP medium. Pathogenicity tests performed with seven selected isolates, including the two pectolytic isolates, on young leaves of grapes cv. Fanny resulted in similar symptoms to those of the plants from which they originated and inoculated bacteria could be re-isolated, confirming Koch's postulates. Using phenotypic tests, analysis of 16S rDNA, housekeeping genes sequences and PCR tests for syrB and syrD genes presence, five of the isolates were identified as Pseudomonas syringae pv. syringae, and the two pectolytic isolates as Xanthomonas arboricola.

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Section: Resultscontrasting

confidence: 99%

Bacterial etiology of necrotic spots on leaves and shoots of grapevine (Vitis vinifera L.) in Poland

et al. 2020

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“…Hort16A in Saga Prefecture since 2010. The evolution of various Japanese lineages different from the strains reported in the past is also supported by results obtained recently by Ferrante and colleagues [ 76 ] using rep-PCR analysis as well as variation in phaseolotoxin gene cluster and type III gene effectors.…”

Section: Discussionsupporting

confidence: 83%

Development of a Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA) to Unravel the Intra-Pathovar Structure of Pseudomonas syringae pv. actinidiae Populations Worldwide

et al. 2015

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The bacterial canker of kiwifruit by Pseudomonas syringae pv. actinidiae is an emblematic example of a catastrophic disease of fruit crops. In 2008 a new, extremely virulent form of the pathogen emerged and rapidly devastated many Actinidia spp. orchards all over the world. In order to understand differences in populations within this pathovar and to elucidate their diffusion and movements on world scale, it is necessary to be able to quickly and on a routine basis compare new isolates with previous records. In this report a worldwide collection of 142 strains was analyzed by MLVA, chosen as investigative technique for its efficacy, reproducibility, simplicity and low cost. A panel of 13 Variable Number of Tandem Repeats (VNTR) loci was identified and used to describe the pathogen population. The MLVA clustering is highly congruent with the population structure as previously established by other molecular approaches including whole genome sequencing and correlates with geographic origin, time of isolation and virulence. For convenience, we divided the VNTR loci in two panels. Panel 1 assay, using six loci, recognizes 23 different haplotypes, clustered into ten complexes with highest congruence with previous classifications. Panel 2, with seven VNTR loci, provides discriminatory power. Using the total set of 13 VNTR loci, 58 haplotypes can be distinguished. The recent hypervirulent type shows very limited diversity and includes, beside the strains from Europe, New Zealand and Chile, a few strains from Shaanxi, China. A broad genetic variability is observed in China, but different types are also retrievable in Japan and Korea. The low virulent strains cluster together and are very different from the other MLVA genotypes. Data were used to generate a public database in MLVAbank. MLVA represents a very promising first-line assay for large-scale routine genotyping, prior to whole genome sequencing of only the most relevant samples.

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“…However, in the latter study a relevant genetic diversity and existence of different populations were found when the recent outbreak strains of P. syringae pv. actinidiae were compared to those isolated in the past in Japan (Ferrante et al ., ). The homogeneity of the strains here is different from the data presented by Kałużna et al .…”

Section: Discussionmentioning

confidence: 97%

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

Abdellatif¹,

Kałużna

Janse

et al. 2017

Plant Pathology

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In the spring of 2012, symptoms of a disease resembling citrus blast and citrus black pit were observed in some orchards in Tunisia. The epidemic spread rapidly in the following years. Twenty‐four commercial citrus orchards from four Tunisian regions showing characteristic symptoms of bacterial diseases were surveyed during a 3‐year study. Eighty‐eight Pseudomonas‐like bacterial isolates were successfully obtained from the northeast and west of Tunisia. No isolates were recovered from the central region. Overall, 46 isolates were identified as Pseudomonas syringae pv. syringae and most of them showed similar phenotypic and genetic profiles. The virulence of three selected isolates differed from one plant cultivar to another as well as from the type of plant organ used for the inoculation. In a bioassay test, all isolates produced syringomycin, which was confirmed by molecular detection based on the syrB and syrD genes. Only EC122 possessed syrD but not syrB. DNA fingerprints, based on repetitive sequence‐based polymerase chain reaction (rep‐PCR) and PCR melting profile (PCR MP), were used to determine the potential genetic diversity among strains. Clustering of PCR MP fingerprinting data matched with rep‐PCR fingerprinting data. The generated distribution tree showed that Tunisian isolates were closely related to the citrus reference strain LMG5496. In contrast, EC112, isolated from citrus, and the almond isolate EC122 were distantly related to the type strain LMG1247T isolated from lilac. Such studies have not been reported until now for P. syringae from citrus.

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Pseudomonas syringae pv. actinidiae strains isolated from past and current epidemics to Actinidia spp. reveal a diverse population structure of the pathogen

Cited by 16 publications

References 38 publications

Bacterial etiology of necrotic spots on leaves and shoots of grapevine (Vitis vinifera L.) in Poland

Bacterial etiology of necrotic spots on leaves and shoots of grapevine (Vitis vinifera L.) in Poland

Development of a Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA) to Unravel the Intra-Pathovar Structure of Pseudomonas syringae pv. actinidiae Populations Worldwide

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

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