Pseudomonas putida infection in rainbow trout

Altınok, İlhan; Kayış, Şevki; Çapkın, Erol

doi:10.1016/j.aquaculture.2006.09.009

Cited by 93 publications

(77 citation statements)

References 14 publications

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“…The results in the Table (1) showed that, all Pseudomonas and Aeromonas species are characterized by Gram-negative motile rods with cytochrome oxidase and those findings agree with Altinok et al (2006); El-Barbary (2010a,b); Nathan et al (2011) whose reported that Pseudomonas species are Gram negative, oxidase positive, rod shaped bacteria that exist in the environment. P. fluorescens showed variation in some phenotypic tests such as gelatin hydrolysis where it has ability to hydrolyze gelatin in contrast to P. putida (Krieg and Holt, 1984).…”

Section: Discussionsupporting

confidence: 80%

“…Some of these results of antibiogram sensitivity are in agreement with those of Eissa et al (2010) who reported that P. anguilliseptica, P. putida and P. aureginosa revealed that almost all of them were sensitive to Gentamicin, Erythromycin Novobiocin and Sulfa-trimethoprime. Also, Altinok et al (2006) reported that P. putida has intrinsically high resistance to Chloramphenicol, Ampicillin, Erythromycin, Tetracycline, Rifampicin, Naladixic acid and Streptomycin. In another study, Darak and Barde (2015) reported that P. fluorescens was very sensitive to Kanamycin, Nalidixic acid, Gentamicin, Neomycin and less sensitive to Amikacin and Tetracycline, and Chlorophenicol.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

El‐Barbary¹,

Hal²

2016

Egypt. J. of Aquatic Biolo. and Fish.

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The current study was carried out to isolate and identify some of bacteria infecting fish of El-Serw fish farm during the harvest season from December to March 2014-2015. Fish samples (155) were collected from different ponds of farm; 65 Nile tilapia (Oreochromis niloticus), 55 catfish (Clarias gariepinus) and 35 mullet (Liza ramada) were used to isolate bacteria in fish organs (gills, liver and kidney) using different selective media, from the examined fish, bacteria belonging to Pseudomonas, Aeromonas and Enterobacteriaceae were isolated. The group most frequently isolated from fish was Aeromonas, the highest number of putative Aeomonas isolates were obtained from O. niloticus 19 (29%), followed C. gariepinus 12 (21.8%) and L. ramada 5 (14.2%). Whereas putative Pseudomonas sp. showed fewer prevalence 9 (13.8%), 8 (14.5%) and 4 (11.4%) from O. niloticus, C. gariepinus and L. ramada, respectively, while only two colonies were recovered from Yersinia Agar Base. Seven isolates were selected and identified by sequencing of 16S rRNA. The analysis of sequence of 16S rRNA gene using universal primers (RW01 and DG74) resulted in the identification of three bacterial isolates of Pseudomonas fluorescens, one of Pseudomonas putida, two of Aeromonas hydrophila and one of Klebsiella oxytoca. The antibiogram test of these isolates revealed their sensitivity to ciprofloxacin, norfloxacin, and gentamycin.This study concluded that the performance 16S rRNA assay has the prospective to create an important contribution to infection management providing rapid of identification the bacterial pathogens in fish.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

El‐Barbary¹,

Hal²

2016

Egypt. J. of Aquatic Biolo. and Fish.

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“…The higher efficiency that was achieved by the addition of nutrients into seawater was partially due to the gradually increasing population of P. putida SP1 in the environment. As several isolates of P. putida were recently reported to be of pathogenic significance (Bouallèguea et al 2004;Altinok et al 2006), we estimated the pathogenicity of P. putida SP1 to secure its use for bioremediation. The LD 50 of P. putida SP1 to two kinds of important marine fish was 1,000-fold higher than the LD 50 of P. fluorescens TSS (10 6.1 ) ), demonstrating that P. putida SP1 was drastically attenuated in its overall bacterial virulence and potentially was a bacterial strain with low pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Zhang

Chen

Liu

2011

Appl Microbiol Biotechnol

126

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The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 μM HgCl 2 . SP1 was also highly resistant to other metals, including CdCl 2 , CoCl 2 , CrCl 3 , CuCl 2 , PbCl 2 , and ZnSO 4 , and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl 2 and the removal of HgCl 2 by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg 2+ to volatile and relatively inert monoatomic Hg 0 vapor, was around 5.0. LD 50 of P. putida SP1 to flounder and turbot was 1.5× 10 9 CFU. Biofilm developed by P. putida SP1 was 1-to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl 2 contamination over a broad range of pH.

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“…Some species of Pseudomonad particularly P. aeruginosa are opportunistic pathogens that can cause serious and life-threatening infections in immunocompromised and cystic fibrosis patients [2,3]. In aquaculture, P. aeruginosa and P. fluorescens especially are the most frequently isolated opportunistic pathogenic species [4,5] although other species may also be serious opportunistic pathogens, including P. anguilliseptica in eels, Anguilla japonica [6] P. chlororaphis in amago trout, Oncorhynchus rhodurus [7], P. plecoglossicida in ayu, P. altivelis [8] and more recently P. putida in rainbow trout, Oncorhynchus mykiss [9].…”

Section: Introductionmentioning

confidence: 99%

Resistance Determinants of Pseudomonas Species from Aquaculture in Australia

Ndi¹

2012

J Aquac Res Development

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Pseudomonas putida infection in rainbow trout

Cited by 93 publications

References 14 publications

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Resistance Determinants of Pseudomonas Species from Aquaculture in Australia

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