Pseudomonas putida – Induced response in phenolic profile of tomato seedlings (Solanum lycopersicum L.) infected by Clavibacter michiganensis subsp. michiganensis

Aksoy, Hasan Murat; Kaya, Yılmaz; Öztürk, Murat; Seçgin, Zafer; Önder, Hasan; Okumuş, Ahmet

doi:10.1016/j.biocontrol.2016.11.001

Cited by 32 publications

(20 citation statements)

References 41 publications

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“…In Egypt, this disease was detected on tomato plants grown in certain newly reclaimed areas (Abd El-Sayed 2002, 2003. To avoid the heavy application of pesticides in the production of vegetable crops, many biological strategies, such as natural products, antibiotic production, growth promotion, and competition for nutrients, have been suggested to protect the plants against pathogens (Aksoy et al 2017). Plant growth-promoting rhizobacteria (PGPR) are known for disease reduction and growth promotion in crops.…”

Section: Introductionmentioning

confidence: 99%

Biological control of the tomato wilt caused by Clavibacter michiganensis subsp. michiganensis using formulated plant growth-promoting bacteria

Abo-Elyousr

Bagy

Hashem

et al. 2019

Egypt J Biol Pest Control

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Rhizobacteria have been reported as bioagents of bacterial diseases and plant growth promoters. The present in vitro study and greenhouse experiment aimed to evaluate the efficiency of Bacillus subtilis, B. amyloliquefaciens, Pseudomonas fluorescens, and P. aeruginosa for the control of bacterial wilt caused by Clavibacter michiganensis subsp. michiganensis and promoting the growth of tomato plants. Effect of formulated and cell suspensions on disease reduction and the pathogen colonization frequency in plants were assessed. The tested bacterial species were able to inhibit the growth of the causal pathogen in vitro. Under greenhouse conditions, the highest reduction in disease severity was detected in tomato plants treated with formulated B. amyloliquefaciens (74.4%), followed by P. aeruginosa (66.7%), while the lowest reduction occurred in tomato seedlings treated with cell suspensions of P. fluorescens (40%) and B. subtilis (53.3%). The four bacterial species produced siderophores, hydrogen cyanide and indole acetic acid in different concentrations. The study confirmed that the use of the four bacterial species as suspensions or formulations could be applied as future eco-friendly alternatives to the synthetic fungicides for controlling the bacterial wilt of tomato caused by C. michiganensis subsp. michiganensis.

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Section: Introductionmentioning

confidence: 99%

Biological control of the tomato wilt caused by Clavibacter michiganensis subsp. michiganensis using formulated plant growth-promoting bacteria

Abo-Elyousr

Bagy

Hashem

et al. 2019

Egypt J Biol Pest Control

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“…Regarding tomato bacterial canker, several PGPR having antagonistic activities toward Cmm have been isolated and studied ( Amkraz et al, 2010 ; Lanteigne et al, 2012 ; Deng et al, 2015 ; Aksoy et al, 2017 ). Among them, the induction of ISR was only reported for Pseudomonas putida (CKPp9; Aksoy et al, 2017 ). This ISR was accompanied by induction of significant amounts of phenolic compounds, which contributed to the disease reduction.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol Rhizobacterium Pseudomonas sp. 23S Induces Systemic Resistance in Tomato (Solanum lycopersicum L.) Against Bacterial Canker Clavibacter michiganensis subsp. michiganensis

2018

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Tomato bacterial canker disease, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is a destructive disease and has been a serious concern for tomato industries worldwide. Previously, a rhizosphere isolated strain of Pseudomonas sp. 23S showed antagonistic activity toward Cmm in vitro. This Pseudomonas sp. 23S was characterized to explore the potential of this bacterium for its use in agriculture. Pseudomonas sp. 23S possesses ability to solubilize inorganic phosphorus, and to produce siderophores, indole acetic acid, and hydrogen cyanide. The strain also showed antagonistic activity against Pseudomonas syringae pv. tomato DC 3000. A plant assay indicated that Pseudomonas sp. 23S could promote growth of tomato seedlings. The potential of treating tomato plants with Pseudomonas sp. 23S to reduce the severity of tomato bacterial canker by inducing systemic resistance (ISR) was investigated using well characterized marker genes such as PR1a [salicylic acid (SA)], PI2 [jasmonic acid (JA)], and ACO [ethylene (ET)]. Two-week-old tomato plants were treated with Pseudomonas sp. 23S by soil drench, and Cmm was inoculated into the stem by needle injection on 3, 5, or 7 days post drench. The results indicated that plants treated with Pseudomonas sp. 23S, 5 days prior to Cmm inoculation significantly delayed the progression of the disease. These plants, after 3 weeks from the date of Cmm inoculation, had significantly higher dry shoot and root weight, higher levels of carbon, nitrogen, phosphorus, and potassium in the leaf tissue, and the number of Cmm population in the stem was significantly lower for the plants treated with Pseudomonas sp. 23S. From the real-time quantitative PCR (qRT-PCR) analysis, the treatment with Pseudomonas sp. 23S alone was found to trigger a significant increase in the level of PR1a transcripts in tomato plants. When the plants were treated with Pseudomonas sp. 23S and inoculated with Cmm, the level of PR1a and ACO transcripts were increased, and this response was faster and greater as compared to plants inoculated with Cmm but not treated with Pseudomonas sp. 23S. Overall, the results suggested the involvement of SA signaling pathways for ISR induced by Pseudomonas sp. 23S.

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“…Various beneficial microorganisms have been used as biocontrol agents against fungal and mold plant pathogens [ 5 ]. Good examples of microorganisms that are able to antagonize the growth of phytopathogenic fungi and mold include many species of bacteria in the genera Streptomyces , Bacillus, and Pseudomonas [ 6–8 ].…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

Sowanpreecha

Rerngsamran

2018

Mycobiology

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Black rot disease in orchids is caused by the water mold Phytophthora palmivora. To gain better biocontrol performance, several factors affecting growth and antifungal substance production by Pseudomonas aeruginosa RS1 were verified. These factors include type and pH of media, temperature, and time for antifungal production. The results showed that the best conditions for P. aeruginosa RS1 to produce the active compounds was cultivating the bacteria in Luria-Bertani medium at pH 7.0 for 21 h at 37 °C. The culture filtrate was subjected to stepwise ammonium sulfate precipitation. The precipitated proteins from the 40% to 80% fraction showed antifungal activity and were further purified by column chromatography. The eluted proteins from fractions 9–10 and 33–34 had the highest antifungal activity at about 75% and 82% inhibition, respectively. SDS-PAGE revealed that the 9–10 fraction contained mixed proteins with molecular weights of 54 kDa, 32 kDa, and 20 kDa, while the 33–34 fraction contained mixed proteins with molecular weights of 40 kDa, 32 kDa, and 29 kDa. Each band of the proteins was analyzed by LC/MS to identify the protein. The result from Spectrum Modeler indicated that these proteins were closed similarly to three groups of the following proteins; catalase, chitin binding protein, and protease. Morphological study under scanning electron microscopy demonstrated that the partially purified proteins from P. aeruginosa RS1 caused abnormal growth and hypha elongation in P. palmivora. The bacteria and/or these proteins may be useful for controlling black rot disease caused by P. palmivora in orchid orchards.

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Pseudomonas putida – Induced response in phenolic profile of tomato seedlings (Solanum lycopersicum L.) infected by Clavibacter michiganensis subsp. michiganensis

Cited by 32 publications

References 41 publications

Biological control of the tomato wilt caused by Clavibacter michiganensis subsp. michiganensis using formulated plant growth-promoting bacteria

Biological control of the tomato wilt caused by Clavibacter michiganensis subsp. michiganensis using formulated plant growth-promoting bacteria

Biocontrol Rhizobacterium Pseudomonas sp. 23S Induces Systemic Resistance in Tomato (Solanum lycopersicum L.) Against Bacterial Canker Clavibacter michiganensis subsp. michiganensis

Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1

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