Pseudomonas putida Biofilm Depends on the vWFa-Domain of LapA in Peptides-Containing Growth Medium

Abstract

The biofilm of Pseudomonas putida is complexly regulated by several intercellular and extracellular factors. The cell surface adhesin LapA of this bacterium is a central factor for the biofilm and, consequently, the regulation of lapA expression, for example, by Fis. It has been recently shown that peptides in growth media enhance the formation of P. putida biofilm, but not as a source of carbon and nitrogen. Moreover, the peptide-dependent biofilm appeared especially clearly in the fis-overexpression strain, which also has increased LapA. Therefore, we investigate here whether there is a relationship between LapA and peptide-dependent biofilm. The P. putida strains with inducible lapA expression and LapA without the vWFa domain, which is described as a domain similar to von Willebrand factor domain A, were constructed. Thereafter, the biofilm of these strains was assessed in growth media containing extracellular peptides in the shape of tryptone and without it. We show that the vWFa domain in LapA is necessary for biofilm enhancement by the extracellular peptides in the growth medium. The importance of vWFa in LapA was particularly evident for the fis-overexpression strain F15. The absence of the vWFa domain diminished the positive effect of Fis on the F15 biofilm.

Comparative Proteomics Analysis Reveals the Domesticated Lepista Sordida Primordium Differentiation Regulation Mechanism and the Subsequent Different Development Patterns in the Pileus and Stipe

“…The maintenance of telomere capping protein 1 (MDBLsor1_03037) could limit cell division and was essential to the survival of eukaryotic cells (Ngo & Lydall, 2010) [78] . The visual word form area (VWFA) domain-containing proteins (MDBLsor1_02352, MDBLsor1_07454) were necessary for biofilm enhancement by the extracellular peptides in the growth medium (Puhm et al, 2022) [79] . The GPI-anchored wall transfer protein (MDBLsor1_02372) was involved in cell wall biogenesis and remodeling (Bowman et al, 2006) [80] .…”

Becoming settlers: Elements and mechanisms for surface colonization by Pseudomonas putida

“…Some strains of P. putida have been described to produce putisolvin, a surfactant molecule that causes biofilm dispersal (Kuiper et al, 2004) and can contribute to displacing competing bacteria in dual-species biofilms (Gazzola et al, 2019). Recently, a Type IVB secretion system present in P. putida IsoF has been shown to deliver a toxic effector into bacterial competitors of a biofilm in a contact-dependent manner (Purtschert-Montenegro et al, 2022). Also, the differential expression of other biocontrol determinants under planktonic and biofilm growth styles is starting to be unveiled: transcription of genes encoding a Type VI secretion system involved in antibacterial activity appears to be down-regulated by FleQ/FleN in combination with c-di-GMP (Nie et al, 2022).…”

“…A structural and regulatory interplay between adhesins and EPS has been postulated (Gjermansen et al, 2010; Martínez‐Gil et al, 2013; Nilsson et al, 2011), but not studied in fine detail. Recently, a domain within the C‐terminal part of LapA, the von Willebrand factor A‐like domain (vWFa), has been described to be important for biofilm formation under certain growth conditions (Puhm et al, 2022). Since in eukaryotes, vWFa is a glycoprotein, it would be an attractive idea that this domain of LapA may participate in interactions with extracellular polysaccharides.…”

Molecular Mechanisms of Bacterial Communication and Their Biocontrol

“…This coordination is mediated by bacterial communications which are obviously encountered within biofilm. In this Special Issue, two works reveal the roles of AHL-and peptide-signaling molecules in the switching of bacteria between motile behavior and a sessile lifestyle, leading to biofilm formation and maturation [4,20]. Thus, it is known that the Pseudomonas putida's lifestyle is complexly regulated by several cellular factors.…”

“…Among them, its cell surface adhesin LapA is a major determinant for attachment and biofilm formation. Extracellular peptides contained in tryptone growth media enhance P. putida biofilm formation not because they serve as nutrients, but because they impact lapA expression [20]. AHL-based QS networks are also strongly suspected to function as master regulators of biofilm development in many Gram-negative bacteria.…”