Pseudomonas protegens sp. nov., widespread plant-protecting bacteria producing the biocontrol compounds 2,4-diacetylphloroglucinol and pyoluteorin

Ramette, Alban; Frapolli, Michele; Saux, Marion Fischer-Le; Gruffaz, Christelle; Meyer, Jean‐Marie; Défago, Geneviève; Sutra, L.; Moënne‐Loccoz, Yvan

doi:10.1016/j.syapm.2010.10.005

Cited by 303 publications

(229 citation statements)

References 57 publications

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“…11 inhibited up to 70% of Stenocarpella maydis and Stenocarpella macrospora and the filtrates obtained in logarithmic growth phase from the P. fluorescens 16 inhibited 54% of the growth of S. maydis. In addition to siderophore and chitinase production, the observed biological control activity of the isolates is probably also due to the production of several antibiotic compounds, like 2,4-diacetylphloroglucinol and pyoluteorin, as is well known for fluorescent pseudomonads [21,44]. Currently, genomic analysis of the two isolates is in progress, which will demonstrate which biosynthetic gene clusters for antibiotic production and other antagonistic activities are present in the two well water isolates from Algeria.…”

Section: Discussionmentioning

confidence: 95%

“…The isolates S4LiBe and S5LiBe appeared to be related to fluorescent pseudomonads based on 16S rDNA gene similarity analysis ( Figure 1) and carbon utilization pattern (Table S1). The isolates S4LiBe and S5LiBe are very similar (99.8% 16SrRNA gene similarity) to each other and are very closely related (99.4 to 99.7%) to the P. protegens strains CHA0 T and Pf-5 [44]. However, a definite phylogenetic clarification would need further analysis based on concatenated alignments of several household genes [30,42], because 16S rRNA analysis alone cannot conclusively resolve very closely related species, like in the Pseudomonas fluorescens cluster.…”

Section: Discussionmentioning

confidence: 99%

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Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Bensidhoum

Nabti

Tabli

et al. 2016

European Journal of Soil Biology

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The application of plant growth-promoting bacteria (PGPB) with biocontrol activities as inoculants of crops plants against phytopathogenic fungi and insect pests provides a biological alternative to the use of agrochemicals. Two Pseudomonas sp. strains were isolated from agricultural well water in the area of Bejaia, northeastern Algeria, located rather closely to a lead mine deposit. The isolates S4LiBe and S5LiBe had 16S rRNA gene sequence similarities of 99.4% to 99.7% with Pseudomonas protegens CHAO T and other P. protegens strains. The phenotypic profiles tested with BIOLOG-GN2-microplates were very similar, but showed also some remarkable differences. The isolates S4LiBe and S5LiBe showed plant growthpromoting potential based on the production of the phytohormone indole acetic acid and siderophores and the solubilization of insoluble phosphate. In addition, they produced chitinase and other polymer degrading enzymes. Interestingly, while S4LiBe and S5LiBe were resistant against heavy metals (2.0 mM K2Cr2O7 and 3.0 mM CoSO4, HgSO4, CdSO4 8H2O and PbCl2), the reference strain P. protegens CHAO T was very sensitive to Hg 2+ and Cd 2+ and had lower tolerance towards Co 2+ and Pb 2+ . The isolates S4LiBe and S5LiBe were very active in mycelial growth inhibition assays against Botrytis cinerea, Verticillium dahlia, Fusarium graminearum, Aspergillus niger and A. flavus (growth inhibition between 88% and 48%). Furthermore, S4LiBe and S5LiBe showed effective insecticidal activities, when tested in the Galleria injection assay and they were tested positive for the insect toxin gene fitD alike the reference strain CHA0 T . Finally, inoculation of barley seeds with S5LiBe resulted in significantly stimulated germination rate and growth of seedlings, with increased shoot length, shoot and root fresh weight, shoot and root dry weight as compared to non-inoculated plants.Thus, the heavy metal tolerant isolates S4LiBe and S5LiBe harbor a diverse potential as beneficial bacteria for agricultural application. They may be very useful even in polluted soils for the stimulation of e.g. biomass crops. The demonstration of successful isolation from agricultural well water may open more ready access for a wide variety of this kind of beneficial bacteria for agricultural application.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Bensidhoum

Nabti

Tabli

et al. 2016

European Journal of Soil Biology

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“…S1 in the supplemental material). The clear demarcation, however, of subgroup 1d strains from other DAPG-producing fluorescent pseudomonads observed in other studies based on genetic and phenotypic traits (8,18,31) led to a comprehensive taxonomic study on CHA0/Pf-5-like strains, for which the name Pseudomonas protegens was suggested (32). In the present study, the phylogenetic analysis of the pqqC gene of this species and its greater ability to solubilize phosphate than that of other strains (see below) points to the same conclusion with respect to the particular position of this group within the fluorescent pseudomonads.…”

Section: Discussionmentioning

confidence: 97%

“…Previous studies on the occurrence, diversity, and evolution of plant-associated Pseudomonas spp. have focused mostly on the diversity of the 16S rRNA (4,31,33), other housekeeping genes (4,8,32), or biocontrol-relevant genes involved in the suppression of various plant pathogenic fungi (6,8,9,18,31,33,40). In addition, little is known about the occurrence of pqq genes among Pseudomonas species.…”

Section: Genes Pqqa -B -C -D -E -F -H -I -J -K and Pqqm (5)mentioning

confidence: 99%

“…Scale bar ϭ 0.02 substitutions per site. Thin-lined box, P. syringae group; dashed-lined box, P. chlororaphis group; and thick-lined box, subgroup 1d (containing strains described as P. protegens by Ramette et al [32]). Capital letters following the names of DAPG-producing strains indicate the multilocus group defined by Frapolli et al (8).…”

Section: Soil Bacteria This Studymentioning

confidence: 99%

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Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Meyer

Frapolli

Keel

et al. 2011

Appl Environ Microbiol

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Many root-colonizing pseudomonads are able to promote plant growth by increasing phosphate availability in soil through solubilization of poorly soluble rock phosphates. The major mechanism of phosphate solubilization by pseudomonads is the secretion of gluconic acid, which requires the enzyme glucose dehydrogenase and its cofactor pyrroloquinoline quinone (PQQ). The main aim of this study was to evaluate whether a PQQ biosynthetic gene is suitable to study the phylogeny of phosphate-solubilizing pseudomonads. To this end, two new primers, which specifically amplify the pqqC gene of the Pseudomonas genus, were designed. pqqC fragments were amplified and sequenced from a Pseudomonas strain collection and from a natural wheat rhizosphere population using cultivation-dependent and cultivation-independent approaches. Phylogenetic trees based on pqqC sequences were compared to trees obtained with the two concatenated housekeeping genes rpoD and gyrB. For both pqqC and rpoD-gyrB, similar main phylogenetic clusters were found. However, in the pqqC but not in the rpoD-gyrB tree, the group of fluorescent pseudomonads producing the antifungal compounds 2,4-diacetylphloroglucinol and pyoluteorin was located outside the Pseudomonas fluorescens group. pqqC sequences from isolated pseudomonads were differently distributed among the identified phylogenetic groups than pqqC sequences derived from the cultivation-independent approach. Comparing pqqC phylogeny and phosphate solubilization activity, we identified one phylogenetic group with high solubilization activity. In summary, we demonstrate that the gene pqqC is a novel molecular marker that can be used complementary to housekeeping genes for studying the diversity and evolution of plant-beneficial pseudomonads.

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Genetics and Evolution of 2, 4‐Diacetylphloroglucinol Synthesis inPseudomonas Fluorescens

Troppens

Moynihan

Barret

et al. 2013

Molecular Microbial Ecology of the Rhizosphere

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Pseudomonas protegens sp. nov., widespread plant-protecting bacteria producing the biocontrol compounds 2,4-diacetylphloroglucinol and pyoluteorin

Cited by 303 publications

References 57 publications

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Heavy metal tolerant Pseudomonas protegens isolates from agricultural well water in northeastern Algeria with plant growth promoting, insecticidal and antifungal activities

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Genetics and Evolution of 2, 4‐Diacetylphloroglucinol Synthesis inPseudomonas Fluorescens

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