Pseudomonas kunmingensis sp. nov., an exopolysaccharide-producing bacterium isolated from a phosphate mine

Xie, Fuhong; Ma, Huan; Quan, Shujing; Liu, Dehai; Chen, Guocan; Chao, Yapeng; Qian, Shijun

doi:10.1099/ijs.0.055632-0

Cited by 21 publications

(12 citation statements)

References 26 publications

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“…The first lineage comprises the P. fluorescens , P. syringae , P. lutea , P. putida , P. anguilliseptica and P. stra<1?show=[fo]?>minea groups, whereas the second includes the P. aeruginosa , P. oleovorans , P. oryzihabitans and P. stutzeri groups. The genus Pseudomonas continues to expand and, since 2013, nine novel species isolated from a wide variety of environments have been described: Pseudomonas chengduensis (Tao et al , 2014), P. formosensis (Lin et al , 2013b), P. guangdongensis (Yang et al , 2013), P. guariconensis (Toro et al , 2013), P. guguanensis (Liu et al , 2013), P. helmanticensis (Ramírez-Bahena et al , 2014), P. kunmingensis (Xie et al , 2014), P. punonensis (Ramos et al , 2013) and P. sagittaria (Lin et al , 2013a).…”

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confidence: 99%

Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain

Pascual

García-López

Bills

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain During the course of screening bacterial isolates as sources of as-yet unknown bioactive compounds with pharmaceutical applications, a chemo-organotrophic, Gram-negative bacterium was isolated from a soil sample taken from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain. Strain F-278,770 T was oxidase-and catalase-positive, aerobic, with a respiratory type of metabolism with oxygen as the terminal electron acceptor, non-spore-forming and motile by one polar flagellum, although some cells had two polar flagella. Phylogenetic analysis of the 16S rRNA, gyrB, rpoB and rpoD genes revealed that strain F-278,770 T belongs to the Pseudomonas koreensis subgroup (Pseudomonas fluorescens lineage), with Pseudomonas moraviensis, P. koreensis, P. baetica and P. helmanticensis as its closest relatives. Chemotaxonomic traits such as polar lipid and fatty acid compositions and G+C content of genomic DNA corroborated the placement of strain F-278,770 T in the genus Pseudomonas. DNA-DNA hybridization assays and phenotypic traits confirmed that this strain represents a novel species of the genus Pseudomonas, for which the name Pseudomonas granadensis sp. nov. is proposed. The type strain is F-278,770 T (5DSM 28040 T 5LMG 27940 T ).

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confidence: 99%

Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain

Pascual

García-López

Bills

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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“…Subsequent studies 17 and this work clearly demonstrate that P. chloritidismutans AW-1 and P. stutzeri ATCC 17588 T are distinct species 34 . P. kunmingensis DSM 25974 T 35 was proposed as a distinct species based on a combination of 16S rRNA gene analysis, phenotypic characterisation and DDH values 13 . However, the analysis omitted P. chloritidismutans AW-1 from the comparison.…”

Section: Resultsmentioning

confidence: 99%

“…However, the current phylogeny is subject to considerable debate 12 and many of the named species may be considered as conspecific. For example, Pseudomonas kunmingensis HL22-2T and P. chloritidismutans AW-1T which are closely related to P. stutzeri may not deserve separate species status 13 , 14 .…”

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confidence: 99%

Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

Mahjoubi

Aliyu

Neifar

et al. 2021

Sci Rep

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Bioremediation offers a viable alternative for the reduction of contaminants from the environment, particularly petroleum and its recalcitrant derivatives. In this study, the ability of a strain of Pseudomonas BUN14 to degrade crude oil, pristane and dioxin compounds, and to produce biosurfactants, was investigated. BUN14 is a halotolerant strain isolated from polluted sediment recovered from the refinery harbor on the Bizerte coast, north Tunisia and capable of producing surfactants. The strain BUN14 was assembled into 22 contigs of 4,898,053 bp with a mean GC content of 62.4%. Whole genome phylogeny and comparative genome analyses showed that strain BUN14 could be affiliated with two validly described Pseudomonas Type Strains, P. kunmingensis DSM 25974T and P. chloritidismutans AW-1T. The current study, however, revealed that the two Type Strains are probably conspecific and, given the priority of the latter, we proposed that P. kunmingensis DSM 25974 is a heteronym of P. chloritidismutans AW-1T. Using GC-FID analysis, we determined that BUN14 was able to use a range of hydrocarbons (crude oil, pristane, dibenzofuran, dibenzothiophene, naphthalene) as a sole carbon source. Genome analysis of BUN14 revealed the presence of a large repertoire of proteins (154) related to xenobiotic biodegradation and metabolism. Thus, 44 proteins were linked to the pathways for complete degradation of benzoate and naphthalene. The annotation of conserved functional domains led to the detection of putative genes encoding enzymes of the rhamnolipid biosynthesis pathway. Overall, the polyvalent hydrocarbon degradation capacity of BUN14 makes it a promising candidate for application in the bioremediation of polluted saline environments.

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“…They are highly related to each other (dissimilarity < 0.01%), and very close to Pseudomonas kunmingensis, Pseudomonas knackmussii , and Pseudomonas chloritidismutans sequences. Pseudomonas kunmingensis is an aerobic bacteria able to perform nitrate reduction (Romanenko et al, 2005; Xie et al, 2014). Pseudomonas knackmussii and Pseudomonas chloritidismutans are facultative aerobic bacteria, unable to perform nitrate reduction (Supplementary Material SM4).…”

Section: Resultsmentioning

confidence: 99%

Shift in Natural Groundwater Bacterial Community Structure Due to Zero-Valent Iron Nanoparticles (nZVI)

et al. 2019

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Toxic and persistent contaminants in groundwater are technologically difficult to remediate. Remediation techniques using nanoparticles (NPs) such as nZVI (Zero-Valent Iron) are applicable as in situ reduction or oxidation agents and give promising results for groundwater treatment. However, these NP may also represent an additional contamination in groundwater. The aims of this study are to assess the impact of nZVI on the nitrate-reducing potential, the abundance and the structure of a planktonic nitrate-reducing bacterial community sampled in groundwater from a multicontaminated site. An active nitrate-reducing bacterial community was obtained from groundwater samples, and inoculated into batch reactors containing a carbon substrate, nitrate and a range of nZVI concentrations (from 0 to 70.1 mg Fe.L -1 ). Physical (pH, redox potential), chemical ( concentrations) and biological (DNA, RNA) parameters were monitored during 1 week, as well as nZVI size distribution and mortality of bacteria. Nitrate-reducing activity was temporally stopped in the presence of nZVI at concentrations higher than 30 mg L -1 , and bacterial molecular parameters all decreased before resuming to initial values 48 h after nZVI addition. Bacterial community composition was also modified in all cultures exposed to nZVI as shown by CE-SSCP fingerprints. Surprisingly, it appeared overall that bacteria viability was lower for lower nZVI concentrations. This is possibly due to the presence of larger, less reactive NP aggregates for higher nZVI concentrations, which inhibit bacterial activity but could limit cell mortality. After 1 week, the bacterial cultures were transplanted into fresh media without nZVI, to assess their resilience in terms of activity. A lag-phase, corresponding to an adaptation phase of the community, was observed during this step before nitrate reduction reiterated, demonstrating the community’s resilience. The induction by nZVI of modifications in the bacterial community composition and thus in its metabolic potentials, if also occurring on site, could affect groundwater functioning on the long term following nZVI application. Further work dedicated to the study of nZVI impact on bacterial community directly on site is needed to assess a potential impact on groundwater functioning following nZVI application.

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Pseudomonas kunmingensis sp. nov., an exopolysaccharide-producing bacterium isolated from a phosphate mine

Cited by 21 publications

References 26 publications

Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain

Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain

Genomic characterization of a polyvalent hydrocarbonoclastic bacterium Pseudomonas sp. strain BUN14

Shift in Natural Groundwater Bacterial Community Structure Due to Zero-Valent Iron Nanoparticles (nZVI)

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