Pseudomonas fluvialis sp. nov., a novel member of the genus Pseudomonas isolated from the river Ganges, India

Abstract

A bacterial strain, designated ASS-1, was isolated and identified from a sediment sample of the river Ganges, Allahabad, India. The strain was Gram-stain-negative, formed straw-yellow pigmented colonies, was strictly aerobic, motile with a single polar flagellum, and positive for oxidase and catalase. The major fatty acids were C16 : 1ω7c/ 16 : 1 C16 : 1ω6c, C18 : 1ω7c and C16 : 0. Sequence analysis based on the 16S rRNA gene revealed that strain ASS-1 showed high similarity to Pseudomonas guguanensis CC-G9A (98.2 %), Pseudomonas alcaligenes ATCC 14909 (98.2 %), Pseudomonas oleovorans DSM 1045 (98.1 %), Pseudomonas indolxydans IPL-1 (98.1 %) and Pseudomonas toyotomiensis HT-3 (98.0 %). Analysis of its rpoB and rpoD housekeeping genes confirmed its phylogenetic affiliation and showed identities lower than 93 % with respect to the closest relatives. Phylogenetic analysis based on the 16S rRNA, rpoB, rpoD genes and the whole genome assigned it to the genus Pseudomonas. The results of digital DNA-DNA hybridization based on the genome-to-genome distance calculator and average nucleotide identity revealed low genome relatedness to its close phylogenetic neighbours (below the recommended thresholds of 70 and 95 %, respectively, for species delineation). Strain ASS-1 also differed from the related strains by some phenotypic characteristics, i.e. growth at pH 5.0 and 42 °C, starch and casein hydrolysis, and citrate utilization. Therefore, based on data obtained from phenotypic and genotypic analysis, it is evident that strain ASS-1 should be regarded as a novel species within the genus Pseudomonas, for which the name Pseudomonasfluvialis sp. nov. is proposed. The type strain is ASS-1 (=KCTC 52437=CCM 8778).

Pseudomonas guryensis sp. nov. and Pseudomonas ullengensis sp. nov., isolated from soil

“…[22] and Sudan et al . [26] respectively. DNA G+C contents for reference species were taken from the GenBank genome database (www.ncbi.nlm.nih.gov/genome/).…”

Pseudomonas yangonensis sp. nov., isolated from wound samples of patients in a hospital in Myanmar

“…The sequences of four housekeeping genes, 16S rRNA, gyrB, rpoB and rpoD, were obtained from the de novo assembled draft genomes. The 16S rRNA sequences of these strains were PCR-amplified using the primers 8F (5′-AGAGTTTGATC-CTGGCTCAG-3′) and 1541R (5′-AAGGAGGTGATC-CAGCCGCA-3′), and the resulting products were sequenced on an ABI 3500XL DNA sequencer (Applied Biosystems), as described previously [8,9]. .…”

Bacteria Analysis in the Ciwalen River Garut using Next Generation Sequencing of 16S Ribosomal RNA

“…Bakteri Paludibacter propionicigenes ditemukan di sawah di Jepang sebagai bakteri gram negatif, anaerobik yang dapat menurunkan nitrat, katalase, dan oksidase (Ueki et al, 2006). Pseudomonas fluvialis adalah bakteri aerob gram negatif yang ditemukan oleh Sudan et al (2018) dalam sampel sedimen dari Sungai Gangga di India. Thauera mechernichensis adalah bakteria dalam genus Thauera.…”