Pseudomonas fluorescens: A Potential Biocontrol Agent for Management of Fungal Diseases of Crop Plants

Majumder, Dipali; Kongbrailatpam, J. D.; Suting, E. G.; Kangjam, Binalata; Lyngdoh, Demandson

doi:10.1007/978-1-4939-1188-2_11

Cited by 2 publications

(2 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of the P. fluorescens bacteria are natural inhabitants of plant roots and soil. An increasing number of P. fluorescens strains are being studied due to their ability to produce secondary metabolites, such as antibiotics, volatile compounds, HCN, siderophores, cell wall degrading enzymes, and phytohormones ( Majumder et al, 2014 ). As part of our research into the genetic basis for biocontrol capacity of P. fluorescens strain 2P24, we isolated and identified a gene cluster that encoded a typical T3SS from the genomic library.…”

Section: Discussionmentioning

confidence: 99%

Supramolecular Structure and Functional Analysis of the Type III Secretion System in Pseudomonas fluorescens 2P24

Liu

Zhang

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

The type III secretion system (T3SS) of plant and animal bacterial pathogens directs the secretion and injection of proteins into host cells. Some homologous genes of T3SS were found also in non-pathogenic bacteria, but the organization of its machinery and basic function are still unknown. In this study, we identified a T3SS gene cluster from the plant growth-promoting Pseudomonas fluorescens 2P24 and isolated the corresponding T3SS apparatus. The T3SS gene cluster of strain 2P24 is similar organizationally to that of pathogenic P. syringae, except that it lacks the regulator hrpR and the hrpK1 and hrpH genes, which are involved in translocation of proteins. Electron microscopy revealed that the T3SS supramolecular structure of strain 2P24 was comprised of two distinctive substructures: a long extracellular, filamentous pilus, and a membrane-embedded base. We show that strain 2P24 deploys a harpin homolog protein, RspZ1, to elicit a hypersensitive response when infiltrated into Nicotiana tabacum cv. xanthi leaves with protein that is partially purified, and by complementing the hrpZ1 mutation of pHIR11. The T3SS of strain 2P24 retained ability to secrete effectors, whereas its effector translocation activity appeared to be excessively lost. Mutation of the rscC gene from 2P24 T3SS abolished the secretion of effectors, but the general biocontrol properties were unaffected. Remarkably, strain 2P24 induced functional MAMP-triggered immunity that included a burst of reactive oxygen species, strong suppression of challenge cell death, and disease expansion, while it was not associated with the secretion functional T3SS.

show abstract

Section: Discussionmentioning

confidence: 99%

Supramolecular Structure and Functional Analysis of the Type III Secretion System in Pseudomonas fluorescens 2P24

Liu

Zhang

et al. 2016

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Currently, the whole genomes of several P. fluorescens strains that exhibit antifungal activity, such as P. fluorescens strains Pf-5, CHA0, F113, SS101, KD, and LBUM223 ( 2 – 5 ), have been sequenced. P. fluorescens strains Pf-5, CHA0, and F113 produce a spectrum of antibiotics, such as 2,4-diacetylphloroglucinol (DAPG) ( 4 , 6 , 7 ), hydrogen cyanide (HCN) ( 8 ), and pyoluteorin ( 2 , 9 ), which are active against various phytopathogenic fungi ( 10 ). The type III secretion system (T3SS) is involved in inhibition of Pythium ultimum by strain KD ( 3 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Pseudomonas fluorescens Strain TR3, a Potential Biocontrol Agent against the Rice Blast Fungus Magnaporthe oryzae

Zhang

Nakatsu

2017

Genome Announc

View full text Add to dashboard Cite

show abstract

Pseudomonas fluorescens: A Potential Biocontrol Agent for Management of Fungal Diseases of Crop Plants

Cited by 2 publications

References 115 publications

Supramolecular Structure and Functional Analysis of the Type III Secretion System in Pseudomonas fluorescens 2P24

Supramolecular Structure and Functional Analysis of the Type III Secretion System in Pseudomonas fluorescens 2P24

Draft Genome Sequence of Pseudomonas fluorescens Strain TR3, a Potential Biocontrol Agent against the Rice Blast Fungus Magnaporthe oryzae

Contact Info

Product

Resources

About