Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry

Kałużna, Monika; Willems, Anne; Pothier, Joël F.; Ruinelli, Michela; Sobiczewski, P.; Puławska, Joanna

doi:10.1016/j.syapm.2016.05.005

Cited by 45 publications

(24 citation statements)

References 46 publications

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“…For some of them they were very aggressive on cantaloupe and could be involved in outbreaks of such crops if grown near citrus orchards (Table 3 ). The species P. cerasi is closely related to P. syringae (PG02b) and was first described with strains isolated from diseased tissue of cherry trees in Poland (Kaluzna et al 2016 ). However, this species potentially included strains of the PG02a phylogroup, isolated from wild plant (Hirano and Upper 1990 ), rain and irrigation basin (Berge et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

et al. 2020

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The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.

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Section: Discussionmentioning

confidence: 99%

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

et al. 2020

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“…A similar hypothesis was made for Pss isolates from mango (Aiello et al, 2015) and P. cerasi sp. nov. isolates from sour cherry (Kału_ zna et al, 2016).…”

Section: Black Pits On Fruits and Blasts On Leaves And Twigs Ofmentioning

confidence: 99%

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

Abdellatif¹,

Kałużna

Janse

et al. 2017

Plant Pathology

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In the spring of 2012, symptoms of a disease resembling citrus blast and citrus black pit were observed in some orchards in Tunisia. The epidemic spread rapidly in the following years. Twenty‐four commercial citrus orchards from four Tunisian regions showing characteristic symptoms of bacterial diseases were surveyed during a 3‐year study. Eighty‐eight Pseudomonas‐like bacterial isolates were successfully obtained from the northeast and west of Tunisia. No isolates were recovered from the central region. Overall, 46 isolates were identified as Pseudomonas syringae pv. syringae and most of them showed similar phenotypic and genetic profiles. The virulence of three selected isolates differed from one plant cultivar to another as well as from the type of plant organ used for the inoculation. In a bioassay test, all isolates produced syringomycin, which was confirmed by molecular detection based on the syrB and syrD genes. Only EC122 possessed syrD but not syrB. DNA fingerprints, based on repetitive sequence‐based polymerase chain reaction (rep‐PCR) and PCR melting profile (PCR MP), were used to determine the potential genetic diversity among strains. Clustering of PCR MP fingerprinting data matched with rep‐PCR fingerprinting data. The generated distribution tree showed that Tunisian isolates were closely related to the citrus reference strain LMG5496. In contrast, EC112, isolated from citrus, and the almond isolate EC122 were distantly related to the type strain LMG1247T isolated from lilac. Such studies have not been reported until now for P. syringae from citrus.

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“…Until that moment, species characterizations and proposals have been performed using physiological, biochemical, serological, and pathological traits. Later, several other species closely related to P. syringae were proposed and validated: Pseudomonas meliae (Ogimi, 1977 ), Pseudomonas savastanoi (Gardan et al, 1992 ), Pseudomonas ficuserectae (Goto, 1983 ), Pseudomonas avellanae (Janse et al, 1996 ), Pseudomonas cannabina (Gardan et al, 1999 ), Pseudomonas tremae (Gardan et al, 1999 ), Pseudomonas congelans (Behrendt et al, 2003 ), Pseudomonas asturiensis (González et al, 2013 ), Pseudomonas cerasi (Kałuzna et al, 2016 ), and Pseudomonas caspiana (Busquets et al, 2017 ). The P. syringae species complex is usually considered to include all these taxonomically closely related species.…”

Section: Introductionmentioning

confidence: 99%

Clarification of Taxonomic Status within the Pseudomonas syringae Species Group Based on a Phylogenomic Analysis

et al. 2017

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The Pseudomonas syringae phylogenetic group comprises 15 recognized bacterial species and more than 60 pathovars. The classification and identification of strains is relevant for practical reasons but also for understanding the epidemiology and ecology of this group of plant pathogenic bacteria. Genome-based taxonomic analyses have been introduced recently to clarify the taxonomy of the whole genus. A set of 139 draft and complete genome sequences of strains belonging to all species of the P. syringae group available in public databases were analyzed, together with the genomes of closely related species used as outgroups. Comparative genomics based on the genome sequences of the species type strains in the group allowed the delineation of phylogenomic species and demonstrated that a high proportion of strains included in the study are misclassified. Furthermore, representatives of at least 7 putative novel species were detected. It was also confirmed that P. ficuserectae, P. meliae, and P. savastanoi are later synonyms of P. amygdali and that “P. coronafaciens” should be revived as a nomenspecies.

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Pseudomonas cerasi sp. nov. (non Griffin, 1911) isolated from diseased tissue of cherry

Cited by 45 publications

References 46 publications

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

Clarification of Taxonomic Status within the Pseudomonas syringae Species Group Based on a Phylogenomic Analysis

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