Pseudomonas (Burkholderia) pseudomallei in Thailand, 1964-1967: geographic distribution of the organism, attempts to identify cases of active infection, and presence of antibody in representative sera.

Finkelstein, Richard A.; Atthasampunna, Pongsom; Chulasamaya, Monthree

doi:10.4269/ajtmh.2000.62.232

Cited by 34 publications

(36 citation statements)

References 15 publications

(12 reference statements)

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“…6,9 All strains of B. pseudomallei survived for at least 5 days in relatively acidic conditions, although survival at pH 3 varied with the strains tested. B. pseudomallei isolates have been recovered from water sources ranging from pH 2 to 9.…”

Section: Resultsmentioning

confidence: 99%

“…6 B. pseudomallei isolates have been recovered from water sources ranging from pH 2-9. 9 Tong and others 6 showed survival of eight strains of B. pseudomallei isolated from soil and water in China in pH 3 saline for a maximum of 7 days. 6 Other Gram-negative bacteria including Pseudomonas fluorescens have been shown to survive for extended periods of time in distilled water up to 16 years, 10 but extended survival has not been reported previously in salt water.…”

Section: Introductionmentioning

confidence: 99%

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The Survival of Burkholderia pseudomallei in Liquid Media

Robertson¹,

Levy²,

Sagripanti³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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We studied the effect of environmental parameters on the survival of Burkholderia pseudomallei. There was a small increase in bacterial count for up to 28 days in sterilized distilled water or rain water, in water at 20°C or 40°C, and in buffered solutions of pH 4 or higher. Counts of culturable B. pseudomallei declined at pH 3, in the presence of seawater or water with concentrations of 4% salt or higher, and under refrigeration. The morphological appearances of B. pseudomallei changed under conditions that maintained culturable numbers from bacilli to coccoid cells and spiral forms under pH or salt stress. These observations indicate that B. pseudomallei can endure nutrient-depleted environments as well as a wide range of pH, salt concentrations, and temperatures for periods of up to 28 days. The relative stability of B. pseudomallei under these conditions underlines the tenacity of this species and its potential for natural dispersal in water: in surface water collections, in managed water distribution systems, and through rainfall. These survival properties help explain the recent expansion of the known melioidosis endemic zone in Australia and may have played a part in recent melioidosis outbreaks.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Survival of Burkholderia pseudomallei in Liquid Media

Robertson¹,

Levy²,

Sagripanti³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…The colony appearance of the different isolates was variable, ranging from smooth to dry, haemolytic to non-haemolytic on blood agar and lactose fermenting to lactose non-fermenting on MacConkey agar, illustrating the considerable inter-strain and mediumdependent variation in colonial morphology shown by this bacterium. 16,19,20 However incubation of the plates at room temperature for one to four days resulted in wrinkling of the colonies and the typical colony appearance in all isolates. The sweet, earthy smell of the colonies was prominent in some isolates.…”

Section: Pitfalls In the Laboratory Diagnosis Of Melioidosismentioning

confidence: 94%

“…15 In most cases, the definitive diagnosis depends on the isolation and identification of B. pseudomallei from clinical specimens. 16,17 Although B. pseudomallei will grow on most routine laboratory media using standard techniques, isolation rates mirror the availability of laboratory facilities and laboratory experience with this pathogen. 16 All the Sri Lankan strains were isolated on primary culture of specimens from sterile sites.…”

Section: Pitfalls In the Laboratory Diagnosis Of Melioidosismentioning

confidence: 99%

“…16,17 Although B. pseudomallei will grow on most routine laboratory media using standard techniques, isolation rates mirror the availability of laboratory facilities and laboratory experience with this pathogen. 16 All the Sri Lankan strains were isolated on primary culture of specimens from sterile sites. Isolation and identification of B. pseudomallei from non-sterile sites is difficult due to scanty growth which is outnumbered by contamination with commensal flora.…”

Section: Pitfalls In the Laboratory Diagnosis Of Melioidosismentioning

confidence: 99%

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Melioidosis in Sri Lanka: an emerging infection

Corea

Thevanesam

Perera³

et al. 2012

Sri Lankan J Infec Dis

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Melioidosis, a pyogenic infection that presents acutely or as a chronic infection, is caused by the soil-associated bacterium Burkholderia pseudomallei. Infection is acquired by inoculation or inhalation and is more common in patients with underlying chronic disease. It is endemic in the tropical belt. Although Sri Lanka is not considered as a country where melioidosis is endemic, an increasing number of cases have been reported recently. Definitive diagnosis requires the isolation of B. pseudomallei in culture from clinical specimens. However, the laboratory diagnosis of melioidosis in Sri Lanka and other under-resourced countries is limited by a lack of familiarity with the bacterium and a lack of facilities to accurately confirm the identity of the isolate. It is highly likely that melioidosis is under-diagnosed in this country. There is a need to increase awareness of this infection among clinicians and clinical microbiologists and improve laboratory facilities for the selective isolation and accurate identification of B. pseudomallei. In addition, studies are needed to determine the epidemiology of melioidosis in Sri Lanka.

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Melioidosis

2013

Emerging Epidemics

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Pseudomonas (Burkholderia) pseudomallei in Thailand, 1964-1967: geographic distribution of the organism, attempts to identify cases of active infection, and presence of antibody in representative sera.

Cited by 34 publications

References 15 publications

The Survival of Burkholderia pseudomallei in Liquid Media

The Survival of Burkholderia pseudomallei in Liquid Media

Melioidosis in Sri Lanka: an emerging infection

Melioidosis

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