Pseudomonas alcaligenes infection and mortality in cultured Chinese sturgeon, Acipenser sinensis

Xu, Jin; Zeng, Xianhui; Jiang, Nan; Zhou, Yong; Zeng, Lingbing

doi:10.1016/j.aquaculture.2015.04.014

Cited by 31 publications

(29 citation statements)

References 22 publications

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“…In this case, outbreaks of haemorrhagic septicaemia in Chinese sturgeon occurred in the summer in fish kept under a high stocking density. A previous study reported that the optimal water temperature for cultured Chinese sturgeon is 20–22°C; these fish are less active when the temperature exceeds 28°C (Xu et al, ). Meanwhile, a high stocking density has also been described as an immunosuppressive factor for Chinese sturgeon (Zhang, Guo, & Gao, ).…”

Section: Discussionmentioning

confidence: 99%

“…To date, the cultured sturgeons are susceptible to several bacterial diseases such as haemorrhagic septicaemia, which is caused by Aeromonas hydrophila or Aeromonas veronii (Amin et al, ; Cao et al, ; Meng, Xiao, & Zeng, ; Soltani & Kalbassi, ); streptococcosis, which is caused by Streptococcus iniae or Streptococcus dysgalactiae (Deng et al, ; Yang & Li, ), and mycobacteriosis, which is caused by non‐tuberculous mycobacteria (Zhang et al, ). In addition, other pathogenic bacteria were occasionally reported in sturgeons, such as Yersinia ruckeri (Shaowu, Di, Hongbai, & Tongyan, ), Pseudomonas alcaligenes (Xu, Zeng, Jiang, Zhou, & Zeng, ), and Elizabethkingia meningoseptica (Di et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and identification of pathogens causing haemorrhagic septicaemia in cultured Chinese sturgeon (Acipenser sinensis )

Zhang

Huang

et al. 2018

Aquac Res

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Chinese sturgeon (Acipenser sinensis) is an endemic fish species of China listed as a critically endangered (CR) species on the IUCN Red List (since 2010). There was a recent outbreak of a novel disease characterized by haemorrhagic ascites and intestinal and renal haemorrhaging in cultured Chinese sturgeon, which clearly posed a serious threat to this species. Two kinds of pathogenic bacteria were isolated from moribund diseased Chinese sturgeon. Two isolates, AHth-1 and AVth-2, were selected as representative strains and were identified as Aeromonas hydrophila andAeromonas veronii based on their morphology, biochemical characteristics, and a phylogenetic analysis derived from 16S rRNA, gyrB, and rpoD gene sequencing.Experimental infection tests showed that both identified isolates were pathogenic to juvenile Dabry's sturgeon (A. dabryanus) via intraperitoneal injection. Histopathology of Dabry's sturgeon infected with A. hydrophila or A. veronii revealed liver hyperaemia, hepatic, and splenic necrosis, and enteritis. Antimicrobial susceptibility tests showed that both bacterial strains were equally sensitive to fluoroquinolones antibiotics, and to third-and fourth-generation cephalosporins. These results provide a scientific reference for the diagnosis, prevention, and treatment of haemorrhagic septicaemia caused by A. hydrophila or A. veronii in cultured sturgeon. K E Y W O R D SAcipenser sinensis, Aeromonas hydrophila, Aeromonas veronii, haemorrhagic septicaemia

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and identification of pathogens causing haemorrhagic septicaemia in cultured Chinese sturgeon (Acipenser sinensis )

Zhang

Huang

et al. 2018

Aquac Res

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“…(Jeremic et al 2003), Serratia marcescens (Baya et al 1992), Klebsiella spp. (Daskalov et al 1998), Pseudomonas alcaligenes (Xu et al 2015) and Pseudomonas fluorescens (Csaba et al 1984). It was difficult to associate these bacteria with disease in this study, since all disease cases with their involvement occurred as concurrent infections with known pathogens.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

et al. 2018

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The intention of this study was to identify the bacterial pathogens infecting Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish), and to establish the antibiotic susceptibility of fish bacteria in Uganda. A total of 288 fish samples from 40 fish farms (ponds, cages, and tanks) and 8 wild water sites were aseptically collected and bacteria isolated from the head kidney, liver, brain and spleen. The isolates were identified by their morphological characteristics, conventional biochemical tests and Analytical Profile Index test kits. Antibiotic susceptibility of selected bacteria was determined by the Kirby-Bauer disc diffusion method. The following well-known fish pathogens were identified at a farm prevalence of; Aeromonas hydrophila (43.8%), Aeromonas sobria (20.8%), Edwardsiella tarda (8.3%), Flavobacterium spp. (4.2%) and Streptococcus spp. (6.3%). Other bacteria with varying significance as fish pathogens were also identified including Plesiomonas shigelloides (25.0%), Chryseobacterium indoligenes (12.5%), Pseudomonas fluorescens (10.4%), Pseudomonas aeruginosa (4.2%), Pseudomonas stutzeri (2.1%), Vibrio cholerae (10.4%), Proteus spp. (6.3%), Citrobacter spp. (4.2%), Klebsiella spp. (4.2%) Serratia marcescens (4.2%), Burkholderia cepacia (2.1%), Comamonas testosteroni (8.3%) and Ralstonia picketti (2.1%). Aeromonas spp., Edwardsiella tarda and Streptococcus spp. were commonly isolated from diseased fish. Aeromonas spp. (n = 82) and Plesiomonas shigelloides (n = 73) were evaluated for antibiotic susceptibility. All isolates tested were susceptible to at-least ten (10) of the fourteen antibiotics evaluated. High levels of resistance were however expressed by all isolates to penicillin, oxacillin and ampicillin. This observed resistance is most probably intrinsic to those bacteria, suggesting minimal levels of acquired antibiotic resistance in fish bacteria from the study area. To our knowledge, this is the first study to establish the occurrence of several bacteria species infecting fish; and to determine antibiotic susceptibility of fish bacteria in Uganda. The current study provides baseline information for future reference and fish disease management in the country.

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“…The combination of P. synxantha and P. aeruginosa improved growth, survival and the immune responses of western king prawns Penaeus latisulcatus juveniles, while P. chlororaphis enhances resistance of European perch Perca fluviatilis against Aeromonas sobria infection (Sánchez et al 2014). On the contrary, Pseudomonas plecoglossicida (Nakai, 2010), Pseudomonas gessardii (Hamza et al 2018), Pseudomonas alcaligenes (Xu et al 2015), Pseudomonas beatica (Lopez et al 2012), Pseudomonas koreensis (Shahi and Mallik, 2014) and Pseudomonas putida (Mao et al 2012) caused infection in aquaculture. Nakai (2010) and Huang et al (2019) reported on the mass mortality of Ayu Plecoglossus altivelis and large yellow croaker Pseudosciaena crocea resulting from P. plecoglossicida attacks, while P. gessardii and P. alcaligenes facilitated the total mortality of freshwater crayfish Pacifastacus leniusculus (Korkut et al 2018) and Chinese sturgeon Acipenser sinensis (Xu et al 2015), respectively.…”

Section: Resultsmentioning

confidence: 99%

Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae

et al. 2020

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Abstract. Wullur S, Napitupulu H, Wantania LL, Ginting EL, Mamangkey NGF, Smolak R, Ogello E. 2020. Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae. Biodiversitas 21: 5291-5297. This study was conducted for the molecular identification of bacteria species isolated from culture medium of the gold-lipped pearl oyster, Pinctada maxima larvae. The pearl oysters were cultured using live-microalgae (Isochrysis sp) and fish waste diet (FWD) as food sources. Bacteria were isolated from the oyster larvae and identified based on the 16S rRNA gene sequence. The isolated bacteria were grown on agar plates and incubated at 37°C for 24 to 48 hours. Representative colonies of the bacteria were selected and cultured for molecular analysis. The 16S rRNA genes of the bacteria were amplified and the sequences were matched with the NCBI GenBank database. Seven different colonies were observed based on morphological characters. Similarity test by conducting the Basic Local Alignment Search Tool (BLAST) in the NCBI GenBank database, using the 16S rRNA gene sequences showed that the seven isolates colonies possess high similarity to five bacteria species i.e. Pseudomonas pachastrellae, Vibrio alginolyticus, Bacillus filamentosus, Bacillus cereus and Idiomarina fontislapidosi belonging to four different genera i.e. Bacillus, Staphylococcus, Vibrio, and Alteromonas.

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Pseudomonas alcaligenes infection and mortality in cultured Chinese sturgeon, Acipenser sinensis

Cited by 31 publications

References 22 publications

Isolation and identification of pathogens causing haemorrhagic septicaemia in cultured Chinese sturgeon (Acipenser sinensis )

Isolation and identification of pathogens causing haemorrhagic septicaemia in cultured Chinese sturgeon (Acipenser sinensis )

Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda

Molecular identification of bacteria isolated from culture medium of the gold-lipped pearl oyster Pinctada maxima larvae

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