Pseudomonas aeruginosa virulence attenuation by inhibiting siderophore functions

Jeong, Geum-Jae; Khan, F. Nawaz; Khan, Sohail; Tabassum, Nazia; Mehta, Sandeep; Kim, Young‐Mog

doi:10.1007/s00253-022-12347-6

Cited by 24 publications

(11 citation statements)

References 111 publications

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“…Pseudomonas is a genus of ubiquitous Gram-negative γ-proteobacterium, the members of which are known for their capacity to colonize various ecological niches; in addition, they are highly versatile and adaptable [ 1 , 2 , 3 ]. This adaptability is considered to be associated with their diverse and highly elaborate siderophore systems [ 4 , 5 , 6 , 7 ]. Iron is an essential trace element for virtually all living organisms to maintain a normal life as it is involved in several key metabolic processes [ 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

The Gene paaZ of the Phenylacetic Acid (PAA) Catabolic Pathway Branching Point and ech outside the PAA Catabolon Gene Cluster Are Synergistically Involved in the Biosynthesis of the Iron Scavenger 7-Hydroxytropolone in Pseudomonas donghuensis HYS

Wang

Xiao

Gao

et al. 2023

IJMS

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The newly discovered iron scavenger 7-hydroxytropolone (7-HT) is secreted by Pseudomonas donghuensis HYS. In addition to possessing an iron-chelating ability, 7-HT has various other biological activities. However, 7-HT’s biosynthetic pathway remains unclear. This study was the first to report that the phenylacetic acid (PAA) catabolon genes in cluster 2 are involved in the biosynthesis of 7-HT and that two genes, paaZ (orf13) and ech, are synergistically involved in the biosynthesis of 7-HT in P. donghuensis HYS. Firstly, gene knockout and a sole carbon experiment indicated that the genes orf17–21 (paaEDCBA) and orf26 (paaG) were involved in the biosynthesis of 7-HT and participated in the PAA catabolon pathway in P. donghuensis HYS; these genes were arranged in gene cluster 2 in P. donghuensis HYS. Interestingly, ORF13 was a homologous protein of PaaZ, but orf13 (paaZ) was not essential for the biosynthesis of 7-HT in P. donghuensis HYS. A genome-wide BLASTP search, including gene knockout, complemented assays, and site mutation, showed that the gene ech homologous to the ECH domain of orf13 (paaZ) is essential for the biosynthesis of 7-HT. Three key conserved residues of ech (Asp39, His44, and Gly62) were identified in P. donghuensis HYS. Furthermore, orf13 (paaZ) could not complement the role of ech in the production of 7-HT, and the single carbon experiment indicated that paaZ mainly participates in PAA catabolism. Overall, this study reveals a natural association between PAA catabolon and the biosynthesis of 7-HT in P. donghuensis HYS. These two genes have a synergistic effect and different functions: paaZ is mainly involved in the degradation of PAA, while ech is mainly related to the biosynthesis of 7-HT in P. donghuensis HYS. These findings complement our understanding of the mechanism of the biosynthesis of 7-HT in the genus Pseudomonas.

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Section: Introductionmentioning

confidence: 99%

The Gene paaZ of the Phenylacetic Acid (PAA) Catabolic Pathway Branching Point and ech outside the PAA Catabolon Gene Cluster Are Synergistically Involved in the Biosynthesis of the Iron Scavenger 7-Hydroxytropolone in Pseudomonas donghuensis HYS

Wang

Xiao

Gao

et al. 2023

IJMS

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“…Therefore, we could not make a hypothesis on whether pyoverdine production would change over time in the evolved isolates. Pyoverdine is highly implicated in P. aeruginosa pathogenicity [37] so pyoverdine production was assessed using spectrophotometry in the ancestral and evolved lineages.…”

Section: Individual Molecular Phenotypesmentioning

confidence: 99%

Long-term Culturing ofPseudomonas aeruginosain Static, Minimal Nutrient Medium Results in Increased Pyocyanin Production, Reduced Biofilm Production, and Loss of Motility

Cecil,

Ornelas,

Phan

et al. 2024

Preprint

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Pseudomonas aeruginosais a multidrug-resistant opportunistic human pathogen that can survive in many natural and anthropogenic environments. It is a leading cause of morbidity in individuals with cystic fibrosis and is one of the most prevalent pathogens associated with nosocomial infections in the United States. It has been shown that this organism can survive and persist in low nutrient environments such as sink drains and inside the respiratory system of human hosts. How adaptation to these types of environments influences phenotypic traits of this organism has not been well studied. Here we implemented an experimental evolution system designed to imitate these environmental niches and identified phenotypic changes that occurred as a result of adaptation to such environments in six strains ofP. aeruginosawith varying life history and colony phenotypes. We observed that adaptation to low nutrient environments resulted in decreased generation time, reduced cell size, reduced biofilm formation, increased pyocyanin production, and decreased motility. Our work is significant as it allows us to predict how this organism will evolve in hospital and domestic environments and can help us improve treatment options for patients.

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“…Not only that, the effectiveness of antimicrobials in treating P. aeruginosa infection has gradually declined in recent years [ 738 , 739 ]. Hopefully, regulate ferroptosis to intervene in the development of various resistance mechanisms in P. aeruginosa has emerged as a promising treatment option [ 740 ]. P. aeruginosa possesses the capability to express lipoxygenase (pLoxA), which catalyzes the oxidation of host AA-phosphatidylethanolamine (AA-PE) to 15-hydroperoxy-AA-PE (15-Ho-AA-PE), thereby instigating ferroptosis in human bronchial epithelial cells [ 741 ].…”

Section: Ferroptosis In Pathologiesmentioning

confidence: 99%

The mechanism of ferroptosis and its related diseases

Feng,

Tang,

Wang

et al. 2023

Mol Biomed

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Ferroptosis, a regulated form of cellular death characterized by the iron-mediated accumulation of lipid peroxides, provides a novel avenue for delving into the intersection of cellular metabolism, oxidative stress, and disease pathology. We have witnessed a mounting fascination with ferroptosis, attributed to its pivotal roles across diverse physiological and pathological conditions including developmental processes, metabolic dynamics, oncogenic pathways, neurodegenerative cascades, and traumatic tissue injuries. By unraveling the intricate underpinnings of the molecular machinery, pivotal contributors, intricate signaling conduits, and regulatory networks governing ferroptosis, researchers aim to bridge the gap between the intricacies of this unique mode of cellular death and its multifaceted implications for health and disease. In light of the rapidly advancing landscape of ferroptosis research, we present a comprehensive review aiming at the extensive implications of ferroptosis in the origins and progress of human diseases. This review concludes with a careful analysis of potential treatment approaches carefully designed to either inhibit or promote ferroptosis. Additionally, we have succinctly summarized the potential therapeutic targets and compounds that hold promise in targeting ferroptosis within various diseases. This pivotal facet underscores the burgeoning possibilities for manipulating ferroptosis as a therapeutic strategy. In summary, this review enriched the insights of both investigators and practitioners, while fostering an elevated comprehension of ferroptosis and its latent translational utilities. By revealing the basic processes and investigating treatment possibilities, this review provides a crucial resource for scientists and medical practitioners, aiding in a deep understanding of ferroptosis and its effects in various disease situations.

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Pseudomonas aeruginosa virulence attenuation by inhibiting siderophore functions

Cited by 24 publications

References 111 publications

The Gene paaZ of the Phenylacetic Acid (PAA) Catabolic Pathway Branching Point and ech outside the PAA Catabolon Gene Cluster Are Synergistically Involved in the Biosynthesis of the Iron Scavenger 7-Hydroxytropolone in Pseudomonas donghuensis HYS

The Gene paaZ of the Phenylacetic Acid (PAA) Catabolic Pathway Branching Point and ech outside the PAA Catabolon Gene Cluster Are Synergistically Involved in the Biosynthesis of the Iron Scavenger 7-Hydroxytropolone in Pseudomonas donghuensis HYS

Long-term Culturing ofPseudomonas aeruginosain Static, Minimal Nutrient Medium Results in Increased Pyocyanin Production, Reduced Biofilm Production, and Loss of Motility

The mechanism of ferroptosis and its related diseases

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