Pseudomonas aeruginosa septicaemia in burns

Gang, R. K.; Bang, Rameshwar L.; Sanyal, Sudip; Mokaddas, Eiman; Lari, Abdul Reda

doi:10.1016/s0305-4179(99)00042-x

Cited by 62 publications

(41 citation statements)

References 17 publications

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“…D espite recent advances in patient care, bacterial infections remain a leading cause of death in severely burned patients (1)(2)(3). In our previous study (4,5) we demonstrated that burn injury suppresses the production of IFN-␥ after Escherichia coli challenge in a mouse model whereas IL-18 treatment restores the IFN-␥ production, thereby improving the survival of burn-injured mice after infection.…”

mentioning

confidence: 94%

“…In our previous study (4,5) we demonstrated that burn injury suppresses the production of IFN-␥ after Escherichia coli challenge in a mouse model whereas IL-18 treatment restores the IFN-␥ production, thereby improving the survival of burn-injured mice after infection. However, many clinical studies have described Pseudomonas aeruginosa and methicillinresistant Staphylococcus aureus, but not E. coli, as the most common agents of postburn infections (1)(2)(3). P. aeruginosa infection especially has a high mortality rate and remains a major cause of death in burned patients (1,2).…”

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confidence: 99%

“…However, many clinical studies have described Pseudomonas aeruginosa and methicillinresistant Staphylococcus aureus, but not E. coli, as the most common agents of postburn infections (1)(2)(3). P. aeruginosa infection especially has a high mortality rate and remains a major cause of death in burned patients (1,2). To improve the prognosis of burned patients, the potentiation of postburn defense mechanisms against P. aeruginosa infection is therefore considered to be very important.…”

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confidence: 99%

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Restoration of Natural IgM Production from Liver B Cells by Exogenous IL-18 Improves the Survival of Burn-Injured Mice Infected with Pseudomonas aeruginosa

Kinoshita

Shinomiya²,

Ono

et al. 2006

The Journal of Immunology

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Pseudomonas aeruginosa is the most common bacterium of postburn infection. In the present study we investigated the immune mechanism of susceptibility to this type of postburn infection and also examined the efficacy of IL-18 treatment. C57BL/6 mice were challenged with P. aeruginosa on day 7 after burn injury. Although the burn-injured mice showed a poor survival rate after bacterial challenge, they retained their IFN-γ production. The burned mice showed lower serum IgM levels and a poor IgM response following P. aeruginosa challenge in comparison with the sham mice, whereas IL-18 treatment after burn injury (alternate day injections for 1 wk) greatly improved the serum IgM levels, which are P. aeruginosa-independent natural IgM before bacterial challenge, thereby increasing the survival rate after the challenge. IL-18 treatment also induced specific IgM to P. aeruginosa in the sera 5 days after bacterial challenge in the burned mice. Interestingly, CD43+CD5−CD23−B220dim cells, namely B-1b cells, increased in the liver after the IL-18 treatment and were found to actively produce IgM in vitro without any additional stimulation. Furthermore, the IL-18 treatment up-regulated the neutrophil count and the C3a levels in the blood as a result of the increased IgM level, which may thus play a critical role in the opsonization and elimination of any invading bacteria. IL-18 treatment for the burned mice and their resultant natural IgM production were thus found to strengthen the host defense against P. aeruginosa infection.

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confidence: 94%

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confidence: 99%

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confidence: 99%

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Restoration of Natural IgM Production from Liver B Cells by Exogenous IL-18 Improves the Survival of Burn-Injured Mice Infected with Pseudomonas aeruginosa

Kinoshita

Shinomiya²,

Ono

et al. 2006

The Journal of Immunology

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“…8,9 Moreover, S. aureus and P. aeruginosa possess the ability to form biofilms that facilitate the colonization of medical devices, thereby contributing to the spread of drug-resistant strains in a hospital environment. 10,11 Improvement of the bactericidal action of currently used antibiotics, by expanding their spectrum of activity, might be achieved using a strategy offered by nanotechnology in conjunction with nontraditional antibiotic agents such as synthetic cationic lipids, which have great potential in overcoming pathogen resistance.…”

Section: Introductionmentioning

confidence: 99%

Core–shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins

Niemirowicz¹,

Piktel²,

Wilczewska³

et al. 2016

IJN

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Core–shell magnetic nanoparticles (MNPs) are promising candidates in the development of new treatment methods against infections, including those caused by antibiotic-resistant pathogens. In this study, the bactericidal activity of human antibacterial peptide cathelicidin LL-37, synthetic ceragenins CSA-13 and CSA-131, and classical antibiotics vancomycin and colistin, against methicillin-resistant Staphylococcus aureus Xen 30 and Pseudomonas aeruginosa Xen 5, was assessed alone and in combination with core–shell MNPs. Fractional inhibitory concentration index and fractional bactericidal concentration index were determined by microdilution methods. The potential of combined therapy using nanomaterials and selected antibiotics was confirmed using chemiluminescence measurements. Additionally, the ability of tested agents to prevent bacterial biofilm formation was evaluated using crystal violet staining. In most conditions, synergistic or additive effects were observed when combinations of core–shell MNPs with ceragenins or classical antibiotics were used. Our study revealed that a mixture of membrane-active agents such as LL-37 peptide or ceragenin CSA-13 with MNPs potentialized their antibacterial properties and might be considered as a method of delaying and overcoming bacterial drug resistance.

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“…Thus, contamination of wound surfaces by these agents occur frequently (Hota et al, 2009;Macedo and Santos, 2006). This is particularly true for P. aeruginosa, which may grow on the moist surface of burn wounds and is highly pathogenic in thermally injured, immunosuppressed patients (Gang et al, 1999). In addition, health-care workers whose hands become contaminated after touching these surfaces can transfer bacteria to a patient or other sites in the environment (Hota et al, 2009 (Douglas et al, 2001;Komolafe et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Bacteriology of burns and antibiogram in an Iranian burn care center

Beheshti¹,

Zia²

2011

Afr. J. Pharm. Pharmacol.

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Burn patients face an obviously high risk for infections due to the immunocompromising effects of their injury. This study aimed to detect and identify bacteria isolated from patients and hospital environment in the burn unit and determine their antibiogram pattern in response to commonly used antimicrobial agents; in order to give recommendations for management of bacterial infections and drug-resistance. Materials of this study were 100 samples of burn wounds and multiple swab samples of different hospital environments. One hundred and twelve isolates were analyzed, from which there was a single agent in the majority of cases (73.3%). Pseudomonas aeruginosa was the most common isolate (32.2%), followed by Enterobacter spp. (16.9%), coagulase-negative staphylococci (12.5%), Acinetobacter spp. (11.7%), Klebsiella spp. (8.9%), Staphylococcus aureus (7.2%), -hemolytic streptococci (4.4%), and others (6.2%). The most commonly detected isolate from hospital environment was P. aeruginosa (35%) followed by Enterobacter spp., Klebsiella spp., coagulase-negative staphylococci. P. aeruginosa was the most resistant to third-and fourth-generation cephalosporins (100%), whereas other gram-negative bacteria were resistant to ciprofloxacin and cephalosporin (70 to 100%). Restriction in the abuse of antibiotics and establishment of an infection control unit will help lower the incidence of infection.

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Pseudomonas aeruginosa septicaemia in burns

Cited by 62 publications

References 17 publications

Restoration of Natural IgM Production from Liver B Cells by Exogenous IL-18 Improves the Survival of Burn-Injured Mice Infected with Pseudomonas aeruginosa

Restoration of Natural IgM Production from Liver B Cells by Exogenous IL-18 Improves the Survival of Burn-Injured Mice Infected with Pseudomonas aeruginosa

Core–shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins

Bacteriology of burns and antibiogram in an Iranian burn care center

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Pseudomonas aeruginosa septicaemia in burns

Cited by 62 publications

References 17 publications

Restoration of Natural IgM Production from Liver B Cells by Exogenous IL-18 Improves the Survival of Burn-Injured Mice Infected with Pseudomonas aeruginosa

Restoration of Natural IgM Production from Liver B Cells by Exogenous IL-18 Improves the Survival of Burn-Injured Mice Infected with Pseudomonas aeruginosa

Core&ndash;shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins

Bacteriology of burns and antibiogram in an Iranian burn care center

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Core–shell magnetic nanoparticles display synergistic antibacterial effects against <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em> when combined with cathelicidin LL-37 or selected ceragenins