Pseudomonas aeruginosa Produces Low Molecular Weight Molecules that Damage Human Respiratory Epithelium in vitro and Slow Mucociliary Transport in the Guinea Pig Trachea in vivo

Wilson, Robert; Munro, N. C.; Hostie, A.; Rutman, A; Sykes, David A.; Watson, David; Shryock, Thomas R.; Ga, Taylor; Cole, P J

doi:10.1378/chest.95.3_supplement.214s

Cited by 6 publications

(4 citation statements)

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“…PCN in clinical samples (e.g., sputa and P. aeruginosa isolates) has been extensively studied to understand its roles in P. aeruginosa infection among CF patients 12, 16–18 . The concentration of PCN in the airways of CF patients was determined and found in a range between 0.9 and 16.5 ppm 19 , and in vitro secretion of PCN by clinical isolates of P. aeruginosa was also reported 20 . The presence and concentration of PCN in clinical specimens are traditionally determined through extraction of the pigment into chloroform or by high performance liquid chromatography (HPLC) 21 .…”

Section: Introductionmentioning

confidence: 99%

Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples

Chen

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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Pseudomonas aeruginosa can cause major infection in immunocompromised patients, and successful antibiotic treatment of the infection relies on accurate and rapid identification of the infectious agents. Here, we reported a culture-free diagnostic method based on the surface-enhanced Raman spectroscopy (SERS) of pyocyanin (PCN), a major biomarker of P. aeruginosa. This platform can detect PCN as low as 5 ppb or 2.38 × 10−8 mol L−1 in both aqueous solutions and spiked clinical sputum samples. It has also been used to dynamically monitor the excretion of PCN by P. aeruginosa during its growth. The presence of PCN has been detected by SERS in 15 clinical sputum samples, which indicates P. aeruginosa infection, with 95.6% sensitivity and 93.3% specificity. The system can advantageously process multiple specimens rapidly, overcomes the need for bacterial culture and diagnostic microbiology assays, and have widespread implications in the early detection of P. aeruginosa infection.

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Section: Introductionmentioning

confidence: 99%

Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples

Chen

et al. 2014

Nanomedicine: Nanotechnology, Biology and Medicine

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“…It has been demonstrated that several bacteria, including Pseudomonas aeruginosa, S. pneumoniae, H. influenzae and others, produce factors that perturb the mucociliary activity and even cause injury to ciliated epithelia in vitro and in vivo [5][6][7]. Studies with S. pneumoniae, for example, have shown that its toxic protein 'pneumolysin' has distinct cytotoxic and complement-activating properties that are associated with marked ciliary dyskinesia [6].…”

Section: Discussionmentioning

confidence: 99%

“…Studies with S. pneumoniae, for example, have shown that its toxic protein 'pneumolysin' has distinct cytotoxic and complement-activating properties that are associated with marked ciliary dyskinesia [6]. Likewise does P. aeruginosa produce substances which damage human respiratory epithelium in vitro and slow the mucociliary transport in the guinea pig trachea in vivo [7]. Hereby, the mucosal contact times of nasal allergens and bacteria may be prolonged.…”

Section: Discussionmentioning

confidence: 99%

Local Pathogenic Bacteria in Allergic Rhinitis: A Novel Concept of Its Pathogenesis

Glück

Gebbers

2003

ORL

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Background: It is still unclear to what extent microorganisms are involved in the pathogenesis of allergic rhinitis. Objective: Therefore, we examined the mucosal colonization with potential pathogenic bacteria (PPB) of the nasal cavity in allergic and nonallergic subjects. Methods: In an open prospective study of 389 office workers (297 men, mean age 42.5 years, and 92 women, mean age 36.7 years), bacterial swabs were taken selectively from both nasal cavities. Standard skin tests for various aeroallergens and negative control tests were conducted in parallel in these subjects. Results: In the 389 subjects, we found positive skin tests in 58 (15%); 37 of these revealed a high level of sensitivity, whilst the other 21 persons had low to moderate levels. Ninety percent of the 58 sensitive persons had PPB in their nasal cavity while only 36% (119) of the remaining 331 subjects with negative skin tests were shown to have PPB in nasal cultures (p < 0.001). Sixty-four percent of the sensitized subjects with PPB were found to have more than 2 PPB species and a positive correlation with the intensity of the skin reaction. In contrast, only 18% (22) of the 119 nonallergic test persons with PPB had more than 1 PPB species. Conclusions: This finding of an unusually high frequency of nasal PPB in subjects with positive skin tests to aeroallergens may indicate an involvement of PPB in the pathogenesis of allergic rhinitis. Yet, further data are still lacking to support this novel concept.

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“…Ions are monitored at m/z 291 patients (e.g. those with cystic fibrosis) at levels high enough to evoke biological responses [20,21 ].…”

Section: Introductionmentioning

confidence: 99%

Excursions in biomedical mass spectrometry

Taylor

Donnelly

Murray

et al. 1996

Brit J Clinical Pharma

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1Mass spectrometry (MS) has played a vital role in the research of the department of clinical pharmacology for over 25 years. 2MS has been used for trace analysis of endogenous compounds and xenobiotics in plasma and urine, and also for a wide range of structural studies. 3Examples of current applications are reported, including data from gas chromatography‐mass spectrometry (GC‐MS) assays for mevalonic acid, the identification of an antibiotic produced by Pseudomonas aeruginosa which is active against Helicobacter pylori, high pressure liquid chromatography (h.p.l.c)‐electrospray MS studies on steroid sulphates, the aspergillus ciliotoxin, gliotoxin, and ADP ribosyltransferase activity in human polymorphonuclear neutrophil leucocytes (PMNs). The value of electrospray MS in the molecular weight determination of proteins is exemplified by the analysis of human serum amyloid component P.

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Pseudomonas aeruginosa Produces Low Molecular Weight Molecules that Damage Human Respiratory Epithelium in vitro and Slow Mucociliary Transport in the Guinea Pig Trachea in vivo

Cited by 6 publications

References 0 publications

Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples

Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples

Local Pathogenic Bacteria in Allergic Rhinitis: A Novel Concept of Its Pathogenesis

Excursions in biomedical mass spectrometry

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