Pseudomonas aeruginosa PA14 Enhances the Efficacy of Norfloxacin against Staphylococcus aureus Newman Biofilms

Orazi, Giulia; Jean-Pierre, Fabrice; O’Toole, George A.

doi:10.1128/jb.00159-20

Cited by 31 publications

(31 citation statements)

References 62 publications

(47 reference statements)

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“…Inoculation of the layered model containing TSB medium with both bacterial species did not result in stable coexistence of both bacterial species, as S. aureus did not reach bacterial numbers of even 10 5 CFU/ml, while P. aeruginosa CFU/ml was unaffected by coculturing with S. aureus (Figure 4). These findings are in concordance with studies that have shown P. aeruginosa to produce antimicrobial exoproducts effective against S. aureus 24,32–34 …”

Section: Resultssupporting

confidence: 92%

“…These findings are in concordance with studies that have shown P. aeruginosa to produce antimicrobial exoproducts effective against S. aureus . 24 , 32 , 33 , 34 …”

Section: Resultsmentioning

confidence: 99%

“…These findings are in concordance with studies that have shown P. aeruginosa to produce antimicrobial exoproducts effective against S. aureus. 24,[32][33][34] The layered woundlike growth medium contained laked horse blood, cattle serum, and peptone, which is similar to wound-simulating media used in several studies. 20,24,25 According to Thaarup and Bjarnsholt, a suitable medium simulating wound fluid should consist of a meat-based broth combined with mammal serum and red blood cells, because the wound bed is recognized to contain mainly damaged host tissue, plasma and red blood cells.…”

Section: Biofilm Model Developmentmentioning

confidence: 99%

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A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

Chen

Lorenzen

et al. 2021

Wound Repair Regeneration

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Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log 10 -reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests. K E Y W O R D S biofilm, coexistence of Pseudomonas aeruginosa and Staphylococcus aureus, model, test of antibiofilm effect | INTRODUCTIONChronic wounds constitute a large burden on patients, society and healthcare systems, affecting 2% of the population in the developed countries with an estimated 2%-3% of the healthcare budgets related to chronic wounds globally, equalling US $13-15 billion annually. [1][2][3] Bacteria inflict on the healing of chronic wounds, 4 with Pseudomonas aeruginosa being identified as a major pathogen 5 together with Staphylococcus aureus, but the underlying mechanisms on the pathogenicity of wound biofilm are not well understood. 6 Furthermore, the Chen and Lorenzen served as co-first authors.

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Section: Resultssupporting

confidence: 92%

“…These findings are in concordance with studies that have shown P. aeruginosa to produce antimicrobial exoproducts effective against S. aureus . 24 , 32 , 33 , 34 …”

Section: Resultsmentioning

confidence: 99%

Section: Biofilm Model Developmentmentioning

confidence: 99%

See 1 more Smart Citation

A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

Chen

Lorenzen

et al. 2021

Wound Repair Regeneration

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“…While direct contact between bacterial cells was presumed to mediate this effect, other studies identified P. aeruginosa secreted products affecting the resistance of S. aureus to antimicrobial compounds. In particular, secondary metabolites from the 4-hydroxy-2-alkylquinolines (HAQ) family such as HQNO increase the resistance of S. aureus to aminoglycosides notably through the induction of the SCV phenotype i ( Hoffman et al, 2006 ; Mitchell et al, 2010 ; DeLeon et al, 2014 ; Orazi and O’Toole, 2017 ; Orazi et al, 2019 , 2020 ). Interestingly, late P. aeruginosa isolates from CF infections often lack HQNO production but are still able to protect S. aureus from tobramycin.…”

Section: Coexistence Promotes Cooperative Behaviors Between mentioning

confidence: 99%

How Bacterial Adaptation to Cystic Fibrosis Environment Shapes Interactions Between Pseudomonas aeruginosa and Staphylococcus aureus

et al. 2021

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Pseudomonas aeruginosa and Staphylococcus aureus are the two most prevalent bacteria species in the lungs of cystic fibrosis (CF) patients and are associated with poor clinical outcomes. Co-infection by the two species is a frequent situation that promotes their interaction. The ability of P. aeruginosa to outperform S. aureus has been widely described, and this competitive interaction was, for a long time, the only one considered. More recently, several studies have described that the two species are able to coexist. This change in relationship is linked to the evolution of bacterial strains in the lungs. This review attempts to decipher how bacterial adaptation to the CF environment can induce a change in the type of interaction and promote coexisting interaction between P. aeruginosa and S. aureus. The impact of coexistence on the establishment and maintenance of a chronic infection will also be presented, by considering the latest research on the subject.

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“…Treating A. fumigatus cultures with supernatants from PAO1 grown in Romling ASM and SCFM2 would likely lead to different results. The P. aeruginosa AQ HQNO is known to alter S. aureus susceptibility to antibiotics (96). Therefore, differential production of HQNO by P. aeruginosa in different ASM could affect the results of antibiotic susceptibility assays performed in different ASM formulations.…”

Section: Resultsmentioning

confidence: 99%

Commercial porcine gastric mucin contributes to variation in production of small molecule virulence factors byPseudomonas aeruginosawhen cultured in different formulations of artificial sputum medium

Phelan

2021

Preprint

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Artificial sputum medium (ASM) is a class of in vitro bacterial culture medium intended to mimic the nutritional environment of cystic fibrosis (CF) pulmonary mucus. One of the most commonly studied microbes in ASM is Pseudomonas aeruginosa, a prevalent and dangerous pathogen of the CF pulmonary microbiome. Many ASM formulations have been reported in literature, with differing nutrient concentrations and availability. Here, we show that common formulations of ASM yield different phenotypes and chemotypes of P. aeruginosa. Further, we demonstrate that iron in commercial porcine gastric mucin (PGM) is sufficient to alter production of P. aeruginosa siderophores in the chemically defined ASM, synthetic CF medium 1 (SCFM1). These results highlight that the choice of ASM formulation for in vitro investigations of microbial pathogenicity, physiology, and interactions should be carefully considered.IMPORTANCEIn vitro culture media are being developed to resemble the in vivo nutritional environment more closely. These culture media are used to investigate microbial pathogenicity and ecology in environments that are more reflective of disease states. In cystic fibrosis (CF), a number of different artificial sputum media (ASM) formulations have been created to recapitulate the CF lung environment. However, these ASM have different sources and concentrations of nutrients. Here, we cultured Pseudomonas aeruginosa in nine different formulations of ASM. P. aeruginosa is the primary pathogen causing lung infection in CF.We show that different ASM formulations lead to different phenotypes and chemotypes by P. aeruginosa and one component of ASM, mucin, contains high levels of iron, which may affect P. aeruginosa physiology.

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Pseudomonas aeruginosa PA14 Enhances the Efficacy of Norfloxacin against Staphylococcus aureus Newman Biofilms

Cited by 31 publications

References 62 publications

A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings

How Bacterial Adaptation to Cystic Fibrosis Environment Shapes Interactions Between Pseudomonas aeruginosa and Staphylococcus aureus

Commercial porcine gastric mucin contributes to variation in production of small molecule virulence factors byPseudomonas aeruginosawhen cultured in different formulations of artificial sputum medium

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