Pseudomonas aeruginosa isolates from dental unit waterlines can be divided in two distinct groups, including one displaying phenotypes similar to isolates from cystic fibrosis patients

Abstract

Pseudomonas aeruginosa displays broad genetic diversity, giving it an astonishing capacity to adapt to a variety of environments and to infect a wide range of hosts. While many P. aeruginosa isolates of various origins have been analyzed, isolates from cystic fibrosis (CF) patients have received the most attention. Less is known about the genetic and phenotypic diversity of P. aeruginosa isolates that colonize other environments where flourishing biofilms can be found. In the present study, 29 P. aeruginosa isolates from dental unit waterlines and CF patients were collected and their genetic and phenotypes profiles were compared to determine whether environmental and clinical isolates are related. The isolates were first classified using the random amplified polymorphic DNA method. This made it possible to distribute the isolates into one clinical cluster and two environmental clusters. The isolates in the environmental cluster that were genetically closer to the clinical cluster also displayed phenotypes similar to the clinical isolates. The isolates from the second environmental cluster displayed opposite phenotypes, particularly an increased capacity to form biofilms. The isolates in this cluster were also the only ones harboring genes that encoded specific epimerases involved in the synthesis of lipopolysaccharides, which could explain their increased ability to form biofilms. In conclusion, the isolates from the dental unit waterlines could be distributed into two clusters, with some of the environmental isolates resembled the clinical isolates.

Occurrence and biofilm forming ability of Pseudomonas aeruginosa in the water output of dental unit waterlines in a dental center in Alexandria, Egypt

“…5 Results of the present study showed that not all of the 9 P. aeruginosa isolates recovered from the DUWL samples were capable of biofilm formation; the percentage of positive biofilm formers differed non-significantly from one method of detection to the other. The inability of some P. aeruginosa isolates from DUWLs to form biofilms has also been reported by Ouellet et al 24 The presence of P. aeruginosa isolates in DUWL output water despite the failure of some of them to produce biofilm in the different biofilm detection experiments can be explained by the fact that P. aeruginosa is capable of joining already existing biofilms furnished by other species. 25 In the current study, no statistically significant differences were found among, or in-between, the three biofilm detection methods regarding their ability to detect and classify biofilm production by the nine P. aeruginosa isolates.…”

Marine-Source Quorum Quenching Enzyme YtnP to Improve Hygiene Quality in Dental Units

“…Pseudomonas aeruginosa, a major human opportunistic pathogen that causes oral infection and secondary diseases [5], is adapted to DCUW colonization by biofilm formation [6] and tolerance to disinfection reagents [7,8]. Studies have revealed that these resistances were regulated by the auto-inducer initiated bacterial communication called quorum sensing (QS) [9], and the auto-inducer in P. aeruginosa and most Gram negative pathogens were classified as N-acyl homoserine lactones (AHL) [10].…”

Enhancement of pyocyanin production by subinhibitory concentration of royal jelly in Pseudomonas aeruginosa

“…Pseudomonas aeruginosa (P. aeruginosa) is one of the Gramnegative bacilli bacteria which causes nosocomial infections that can be fatal, especially in immunocompromised patients [1][2][3] . These bacteria are often found in the dental unit waterlines which allows the transmission of these bacteria into the oral cavity 4 . As an opportunist pathogen, P. aeruginosa is also frequently involved in oral infections, such as necrotizing ulcerative gingivitis, periodontitis, and mandibular osteomyelitis [5][6][7] .…”