Pseudomonas aeruginosa Infectious Keratitis in a High Oxygen Transmissible Rigid Contact Lens Rabbit Model

Abstract

These data indicate that in the absence of adequate tear clearance, the presence of P. aeruginosa trapped under the lens overrides the protective effects of oxygen on surface epithelial cells. These findings also suggest that alternative pathophysiological mechanisms exist whereby changes under the lens in the absence of frank hypoxic damage result in P. aeruginosa infection in the otherwise healthy corneal epithelium.

A novel murine model for contact lens wear reveals clandestine IL-1R dependent corneal parainflammation and susceptibility to microbial keratitis upon inoculation with Pseudomonas aeruginosa

“…Neutrophil infiltration into the cornea generally causes collateral damage [24,27,54]. However, the Ly6G-positive cell (neutrophil) response during mouse lens wear occurred without associated changes to corneal transparency suggesting an alternate neutrophil phenotype with reduced generation of reactive oxygen species.…”

“…Several studies have reported the use of contact lens-wearing animal models to investigate the pathogenesis of lens-associated microbial keratitis. Examples include cytokine and chemokine profiles of P. aeruginosa -challenged corneas in a lens-wearing rat model [21,22], the association of contact lens-associated biofilms/bacterial adaptations in development of P. aeruginosa keratitis in a lens-wearing rat model [23], the relationship between neutrophil infiltration and severity of P. aeruginosa keratitis in a rabbit model [24], and the role of lens colonization and microbial antigens in lens-induced infiltrative events in a guinea pig model [25]. However, these previous lens-wearing models have been limited by one or more factors: 1) limited lens supply [23], 2) use of a larger animal (e.g.…”

“…However, these previous lens-wearing models have been limited by one or more factors: 1) limited lens supply [23], 2) use of a larger animal (e.g. rabbit) for which eyelid closure or nictitating membrane surgery is required for lens retention [24,26], 3) infiltrative, inflammatory events that occur but do not lead to microbial keratitis (e.g. guinea pig) [25], 4) microbial keratitis that does not occur or is evident at a low level despite repeated bacterial inoculation (e.g.…”

Commonly Used Animal Models

“…Rabbits have been used as a model of human pregnancy and for the production of polyclonal antibodies for use in immunology research (Hanly et al, 1995;Ema et al, 2010;Ito et al, 2011;Fischer et al, 2012). Rabbits are routinely used in (Southard et al, 2000;Arslan et al, 2003;McMahon et al, 2005;Castaneda et al, 2008;Habjanec et al, 2008;Manabe et al, 2008;Xiangdong et al, 2011;Panda et al, 2014;Sriram et al, 2014;Wei et al, 2014;Zhou et al, 2014). The production of polyclonal antibodies is preferentially performed in the rabbit due to its relatively large blood volume compared to rodents (Hanly et al, 1995).…”

Desenvolvimento e caracterização de lipossomas mucoadesivos para liberação ocular de fármacos

“…Em regiões menos desenvolvidas e industrializadas, por exemplo, o risco maior está na população agrária, nas temporadas de colheita, em que o trabalhador passa horas exposto à micro-organismos presentes no solo e nas plantas e que podem ocasionar lesão direta e inoculação destes micro-organismos (BHARATHI et al, 2003;GODOY et al, 2004). Já em regiões mais desenvolvidas e industrializadas, o risco maior está no uso constante de lentes de contato (MARANGON et al, 2004;TODOKORO et al, 2014;WEI et al, 2014). DURAND et al, 2005;EPAULARD et al, 2011;HAGAN et al, 1995;MISRA;MALIK;SINGHAL, ;OZDEMIR et al, 2012;SANATI et al, 1997;STRICTO, 2015).…”