Pseudomonas aeruginosa infection in the hypoventilated middle ear: An experimental model

Trinidad, Almudena; Ramírez‐Camacho, Rafael; García-Berrocal, José Ramón; Verdaguer, José María; Vicente, Javier; Pinilla, Mauricio

doi:10.1080/00016480410022804

Cited by 11 publications

(3 citation statements)

References 19 publications

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“…In all of the above models, animals that demonstrated signs of otitis media and otitis externa were excluded. As there are a number of documented animal models for acute [62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78] and chronic 44,56,62,[79][80][81][82][83][84][85][86][87][88][89][90][91][92][93] otitis media, the key to developing an animal model for chronic perforation may involve perforation in the setting of an existing otitis media model. Myringotomy during acute otitis media prolongs the effects of infection on the tympanic membrane.…”

Section: Discussionmentioning

confidence: 99%

Chronic tympanic membrane perforation: a better animal model is needed

Maria

Atlas

Ghassemifar

2007

Wound Repair Regeneration

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Developments in the treatment of chronic tympanic membrane perforation have been hindered by the lack of an ideal animal model. It is not appropriate to test such treatments on acute perforations as the majority of these heal spontaneously. An ideal animal model would be one that most closely resembles the human clinical situation. It should be inexpensive, readily available, and easy to create. There have been a number of attempts to create a chronic tympanic membrane perforation model with limited success. All published attempts at chronic tympanic membrane perforations have been reviewed and the limitations of each model are discussed. A number of areas for research exist for further developing a chronic tympanic membrane perforation model. These areas include a perforation model in the presence of bacteria and eustachian tube dysfunction. Understanding the molecular and genetic mechanisms of chronic otitis media and potential treatments will also be useful.

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Section: Discussionmentioning

confidence: 99%

Chronic tympanic membrane perforation: a better animal model is needed

Maria

Atlas

Ghassemifar

2007

Wound Repair Regeneration

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“…This concentration has been shown to consistently induce an infection in rats. [7][8][9] The animals were anesthetized by intraperitoneal injection of ketamine hydrochloride (100 mg/kg) and diazepam (0.1 mg/kg). The body temperature was maintained at 37ºC through the use of a rectal thermometer and a temperature-controlled electric blanket.…”

Section: Methodsmentioning

confidence: 99%

“…Fluid was collected from ears with purulent effusion (ie, rats in group C) and cultured by a method previously reported. 7 After sampling, the rats were euthanized. The temporal bones were removed and fixed with 10% formalin for at least 2 days.…”

Section: Methodsmentioning

confidence: 99%

Morphological Sequence of Plastipore Extrusion in Experimental Otitis Media

Trinidad

Vicente²,

Verdaguer³

et al. 2007

Ann Otol Rhinol Laryngol

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Applicability of autologous incus: the influence of age and localization of cholesteatoma

Stanković

2007

Eur Arch Otorhinolaryngol

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There is a correlation between macroscopic and histologic changes of incus in chronic otitis with cholesteatoma. The incidence and types of damage of auditory ossicles in chronic otitis media were extensively studied, but except some studies on reoperations there are no morphometric studies of human auditory ossicles in chronic otitis media. In order to define the rate of ossicular applicability for tympanoplasties in different macroscopic forms of damage, we analysed histologic changes of human incudes in correlation to their macroscopic alterations. Histomorphometric study of 82 intraoperatively removed human incudes, from chronic otitis media with cholesteatoma (attic, sinus, tensa cholesteatoma), was performed. They were divided according to the age of patients and localization of cholesteatoma. Erosions, vitality, vascular areas, osteomyelitic zones, and applicability of ossicles were measured. Progressive increase of erosions, decrease of vitality, dilatation of vascular spaces and osteomyelitic areas, and decrease of rate of potential applicability for tympanoplasties were found. Residual osteomyelitis was present in incudes with advanced external osteitis and ossicular defect. Macroscopic changes of incudes in cholesteatoma and their histologic structure correlate. Localisation of cholesteatoma is important for ossicular damage. Defects of long process with external osteitis of incudes and more intense pathological changes demand their removal and use of other reconstructive materials for tympanoplasties in order to avoid tympanoplasty failure.

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Pseudomonas aeruginosa infection in the hypoventilated middle ear: An experimental model

Abstract: Abnormal otomicroscopic findings and persistence of infection were correlated with the histopathological changes found in middle ear tissues.

Cited by 11 publications

References 19 publications

Chronic tympanic membrane perforation: a better animal model is needed

Chronic tympanic membrane perforation: a better animal model is needed

Morphological Sequence of Plastipore Extrusion in Experimental Otitis Media

Applicability of autologous incus: the influence of age and localization of cholesteatoma

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