Pseudomonas aeruginosa Growth Inhibitor, PAGI264: A Natural Product from a Newly Isolated Marine Bacterium, Bacillus sp. Strain REB264

Bacillus subtilis as a host for natural product discovery and engineering of biosynthetic gene clusters

“…298 Marine-derived Bacillus and Paenibacillus strains in particular represent a rich source of novel chemistry. [299][300][301] Although interesting new secondary metabolites are discovered frequently, [302][303][304][305] research suggests that many Bacillales natural products remain undiscovered. 306,307 Furthermore, it has been shown that Firmicutes species, including many Bacillus strains, are oen underrepresented in metagenomics libraries due to their ability to form endospores, which hinders the efficacy of standard DNA extraction techniques.…”

Exploring Oceans for Curative Compounds: Potential New Antimicrobial and Anti-Virulence Molecules against Pseudomonas aeruginosa

“…Moreover, the genus Bacillus was revealed to be an important source of bioactive molecules against P. aeruginosa , such as the phthalate derivative di-(2-ethylhexyl) phthalate (DEHP) from a marine B. subtilis active at the MIC value of 8 µg/mL [ 153 ], the two novel glycolipids (ieodoglucomide C and ieodoglycolipid) isolated from B. licheniformis 09IDYM23, active at the MIC value of 0.01 and 0.03 µM, respectively [ 154 ], and the compound PAGI264 produced by Bacillus sp. REB264, able to inhibit the growth of P. aeruginosa in microdilution assay with a MIC of 15 µg/mL [ 155 ]. Moreover, macrolactines are a class of compounds mainly produced by Bacillus strains and show antimicrobial activity because of their unique chemical architecture.…”