Pseudomonas aeruginosa as a Powerful Biofilm Producer and Positive Action of Amikacin Against Isolates From Chronic Wounds

Rahim, Kashif; Saleha, Shamim; Basit, Abdul; Zhu, Xudong; Ahmed, Iqbal Ike K.; Liang, Hongbin; Zhang, Ping; Usman, Bakhtawar; Munir, Shahzad; Franco, Octávio L.

doi:10.5812/jjm.57564

Cited by 10 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, MDR and XDR strains were counted as 38.61% and 7.92% of isolates, respectively. These results are in agreement with different studies of Venezuela, 22 China, 23 and Germany, 24 that demonstrated high rates of antibacterial resistance of P. aeruginosa, where they showed similar resistance to the antibiotics examined in this study. In contrast to the current study, some reports showed a low rate of resistance in the last three years, except for colistin, in which the same findings were described.…”

Section: Discussionsupporting

confidence: 93%

Prevalence and Molecular Typing of Colistin-Resistant Pseudomonas aeruginosa (CRPA) Among β-Lactamase-Producing Isolates: A Study Based on High-Resolution Melting Curve Analysis Method

Tahmasebi¹,

Dehbashi²,

Arabestani³

2020

IDR

View full text Add to dashboard Cite

Background: The frequency and production of β-lactamase enzymes may be different in colistin-resistant Pseudomonas aeruginosa (CRPA) strains compared to susceptible strains. The purpose of this study was to investigate the relationship between colistin resistance and β-lactamase enzymes in different Sequence Types (ST) of P. aeruginosa. Methods: A total of 101 P. aeruginosa isolates were collected from different samples. The antimicrobial susceptibilities of the bacterial isolates were examined by disk diffusion and MIC E-test methods. Also, real-time PCR and high-resolution melting curve analysis (HRMA) assay were performed to detect the resistance genes. Results: Out of the 101 P. aeruginosa isolates, four isolates (3.96%) were resistant to colistin. Also, 39 isolates (38.61%) were considered as MDR, and eight isolates (7.92%) were considered as XDR. Further, 25 (24.75%) and 26 isolates (25.74%) were produced ESBL and carbapenemase enzymes, respectively. According to HRMA results, four isolates (3.96%) were positive for pmrA, three isolates (2.97%) were positive for mcr-1, 25 isolates (24.75%) were positive for blaTEM, 24 isolates (23.76%) were positive for blaSHV, 26 isolates (25.75%) were positive for blaKPC, and 23 isolates (22.77%) were positive for blaIMP genes. Furthermore, ST108 and ST250 showed the highest distribution in P. aeruginosa isolates. Also, ST217, ST1078, and ST3340 were reported as novel types in CRPA strains. Conclusion: Concerns about the prevalence of CRPA strains should be taken seriously. Also, our results showed that the mcr-1 gene plays a vital role in the distribution of ESBL and KPC-producing P. aeruginosa strains.

show abstract

Section: Discussionsupporting

confidence: 93%

Prevalence and Molecular Typing of Colistin-Resistant Pseudomonas aeruginosa (CRPA) Among β-Lactamase-Producing Isolates: A Study Based on High-Resolution Melting Curve Analysis Method

Tahmasebi¹,

Dehbashi²,

Arabestani³

2020

IDR

View full text Add to dashboard Cite

show abstract

“…Diabetic foot included the highest number of patients. Rahim K et al reported 7 that diabetic wounds were the highest prevalent (43.96%) wounds infected by P. aeruginosa , which was higher than that reported by the present study (32%). Diabetic foot infection is the most common and severe complication in patients with diabetes.…”

Section: Discussioncontrasting

confidence: 77%

Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on Pseudomonas aeruginosa Isolated from Chronic Wounds

Etemadinia¹,

Seyfoori²,

Foroushani³

et al. 2022

AJMB

View full text Add to dashboard Cite

Background: Biofilm formation helps Pseudomonas ‎aeruginosa (P. ‎aeruginosa) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropyl-acrylamide microgels (PNIPAM) on P. aeruginosa. Methods: Totally‎, ‎100 P. aeruginosa strains were isolated from chronic wound infections‎. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, algD, lasR, and PA2714 genes were amplified to investigate gene frequencies and expression rates. Results: Significant decreases were seen in lasR expression in EDTA-treated samples‎. Significant decreases were observed in expression of algD and lasR treated with xylitol. Decreased ‎expression of PA2714 was seen in samples treated with xylitol with no significance. ‎ Conclusion: The PNIPAM containing xylitol ‎or EDTA could penetrate biofilms of P. aeruginosa and significantly decrease expression of lasR and algD. This can be a novel strategy in the management of chronic wounds.

show abstract

“…Staphylococcus aureus causes many complications -from inflammation of the skin and soft tissues to lethal pneumonia -and is one of the most rapidly spreading "superbugs" [48,49]. Pseudomonas aeruginosa is associated mainly with the development of chronically infected wounds, significantly complicates their therapy due to the ability to actively form barrier biofilms, and is also characterized by increased antibiotic resistance [49,50]. Enterococcus hirae has previously been observed relatively rarely.…”

Section: Research Planningmentioning

confidence: 99%

Broad-purpose antimicrobial chlorine-active polymers: suppression of multidrug-resistant microorganisms and microbial penetration resistance

Murashevych

Koshova

Surmasheva

et al. 2022

SR: PS

View full text Add to dashboard Cite

The aim of the work was to evaluate the antimicrobial activity of polymeric materials with immobilized N-Chlorosulfonamide groups against multidrug-resistant hospital strains of common microorganisms and to determine the resistance to microbial penetration of these materials. Materials and methods: the studied samples were copolymers of styrene with divinylbenzene in the form of staple fibre and non-woven fabric with immobilized N-Chlorosulfonamide groups of various structures. Hospital strains of microorganisms have been isolated from clinical material; their antibiotic sensitivity has been determined by the Kirby-Bauer method. The agar diffusion method determines the antimicrobial activity of the polymers. Resistance to microbial penetration of samples of non-woven fabric has been determined by the membrane filtration method. Results: polymer samples have been synthesized with immobilized N-Chlorosulfonamide groups in the Na- and H-forms, and with the N, N-dichlorosulfonamide group, with chlorine concentration range 3.7 - 12.5 %. All samples demonstrated pronounced antimicrobial activity against both standard and hospital strains. Due to the higher specific surface area, staple fibre is generally more efficient. An increase in the zone of inhibition of the growth of microorganisms was observed with an increase in the concentration of immobilized chlorine. All the studied fabric samples are impermeable to S. aureus. The control samples containing the free sulfonamide group did not show antimicrobial properties. Conclusions: synthesized chlorine-active polymers have a pronounced antimicrobial activity against multidrug-resistant microorganisms, demonstrate high resistance to microbial penetration and therefore are promising for creating a wide range of medical products on their basis: dressings, protective masks, antimicrobial filters, etc.

show abstract

Pseudomonas aeruginosa as a Powerful Biofilm Producer and Positive Action of Amikacin Against Isolates From Chronic Wounds

Cited by 10 publications

References 31 publications

<p>Prevalence and Molecular Typing of Colistin-Resistant <em>Pseudomonas aeruginosa</em> (CRPA) Among β-Lactamase-Producing Isolates: A Study Based on High-Resolution Melting Curve Analysis Method</p>

<p>Prevalence and Molecular Typing of Colistin-Resistant <em>Pseudomonas aeruginosa</em> (CRPA) Among β-Lactamase-Producing Isolates: A Study Based on High-Resolution Melting Curve Analysis Method</p>

Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on Pseudomonas aeruginosa Isolated from Chronic Wounds

Broad-purpose antimicrobial chlorine-active polymers: suppression of multidrug-resistant microorganisms and microbial penetration resistance

Contact Info

Product

Resources

About