Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation

Mowat, Eilidh; Rajendran, Ranjith; Williams, Craig; McCulloch, Elaine; Jones, Brian; Lang, Sue; Ramage, Gordon

doi:10.1111/j.1574-6968.2010.02130.x

Cited by 187 publications

(183 citation statements)

References 33 publications

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“…In a clinical setting, various bacterial small molecules have been shown to affect the morphological transition of fungi from the yeast form to the filamentous form, which is critical in the context of fungal pathogenicity. These include short-chain fatty acids from lactic acid bacteria (282) have also been documented; for example, a recent study indicated that diffusible molecules from P. aeruginosa suppress Aspergillus fumigatus biofilm formation in vitro, which may explain why the fungus causes only low mortality in the lungs of cystic fibrosis patients in which P. aeruginosa is a common inhabitant (262). Effects on bacterial and fungal physiology.…”

Section: Consequences Of Bacterial-fungal Interactions For Participatmentioning

confidence: 99%

Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists

Frey‐Klett

Burlinson

Deveau

et al. 2011

Microbiol Mol Biol Rev

720

531

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SUMMARY Bacteria and fungi can form a range of physical associations that depend on various modes of molecular communication for their development and functioning. These bacterial-fungal interactions often result in changes to the pathogenicity or the nutritional influence of one or both partners toward plants or animals (including humans). They can also result in unique contributions to biogeochemical cycles and biotechnological processes. Thus, the interactions between bacteria and fungi are of central importance to numerous biological questions in agriculture, forestry, environmental science, food production, and medicine. Here we present a structured review of bacterial-fungal interactions, illustrated by examples sourced from many diverse scientific fields. We consider the general and specific properties of these interactions, providing a global perspective across this emerging multidisciplinary research area. We show that in many cases, parallels can be drawn between different scenarios in which bacterial-fungal interactions are important. Finally, we discuss how new avenues of investigation may enhance our ability to combat, manipulate, or exploit bacterial-fungal complexes for the economic and practical benefit of humanity as well as reshape our current understanding of bacterial and fungal ecology.

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Section: Consequences Of Bacterial-fungal Interactions For Participatmentioning

confidence: 99%

Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists

Frey‐Klett

Burlinson

Deveau

et al. 2011

Microbiol Mol Biol Rev

720

531

View full text Add to dashboard Cite

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“…These metabolites could be intermediates in the detoxification process or serve other, as-yet unexplored roles, as exemplified by 1-HP (2), which turned on the production of fungal siderophores. MALDI-IMS also revealed that A. fumigatus converted the P. aeruginosa metabolites PCA (3) and PYO (1) into dimeric phenazines (9,10). The identification of these unique fungal bioconversion metabolites provides opportunities to study their effects on both the producing organism and competing bacterium.…”

Section: Biotransformation Of P Aeruginosa Phenazine Metabolites By Amentioning

confidence: 99%

“…Interestingly, however, in a pulmonary mouse model, mice coinfected with P. aeruginosa and A. fumigatus had a higher survival rate than mice infected by A. fumigatus alone (9). Additional in vitro studies have suggested that P. aeruginosa has an inhibitory effect on filamentation and biofilm formation of A. fumigatus through both direct contact and secreted molecules (10). The coexistence of P. aeruginosa and A. fumigatus in the CF lung, species composition, spatial orientation, and molecular interaction remain to be elucidated, however.…”

mentioning

confidence: 99%

Interkingdom metabolic transformations captured by microbial imaging mass spectrometry

Moree

Phelan

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

221

241

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In polymicrobial infections, microbes can interact with both the host immune system and one another through direct contact or the secretion of metabolites, affecting disease progression and treatment options. The thick mucus in the lungs of patients with cystic fibrosis is highly susceptible to polymicrobial infections by opportunistic pathogens, including the bacterium Pseudomonas aeruginosa and the fungus Aspergillus fumigatus. Unravelling the hidden molecular interactions within such polymicrobial communities and their metabolic exchange processes will require effective enabling technologies applied to model systems. In the present study, MALDI-TOF and MALDI-FT-ICR imaging mass spectrometry (MALDI-IMS) combined with MS/MS networking were used to provide insight into the interkingdom interaction between P. aeruginosa and A. fumigatus at the molecular level. The combination of these technologies enabled the visualization and identification of metabolites secreted by these microorganisms grown on agar. A complex molecular interplay was revealed involving suppression, increased production, and biotransformation of a range of metabolites. Of particular interest is the observation that P. aeruginosa phenazine metabolites were converted by A. fumigatus into other chemical entities with alternative properties, including enhanced toxicities and the ability to induce fungal siderophores. This work highlights the capabilities of MALDI-IMS and MS/MS network analysis to study interkingdom interactions and provides insight into the complex nature of polymicrobial metabolic exchange and biotransformations. M icrobes that colonize mammalian hosts can form polymicrobial communities, such as biofilms, where they establish commensual, mutualistic, competitive, or antagonistic interactions with one another and with the host. In microbial disease, this complex interplay can affect the outcome of antimicrobial therapy (1). Therefore, it is important to understand polymicrobial populations and their interactions at the molecular level.In persons with cystic fibrosis (CF), the lungs are lined with a viscous mucus layer susceptible to polymicrobial infections (2). Pseudomonas aeruginosa, a Gram-negative bacterial opportunistic pathogen, is the most prevalent and persistent microorganism (3) isolated from the sputum of CF lungs and leading cause of mortality in CF patients (4). Within the CF lung, P. aeruginosa exists in biofilm-like macrocolonies (5) and is refractory to antimicrobial agents and the host immune response (6). Aspergillus fumigatus, an opportunistic fungal pathogen, is the second-most persistent microbe in the CF lung, with a 10-57% prevalence rate (3), and is capable of causing allergic bronchopulmonary aspergillosis (7).Superinfection with both P. aeruginosa and A. fumigatus in CF patients leads to decreased pulmonary function compared with monoinfection with either microbe (8). Interestingly, however, in a pulmonary mouse model, mice coinfected with P. aeruginosa and A. fumigatus had a higher survival rate than mice...

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“…One way these organisms interact is through the production and secretion of small-molecule effectors called specialized metabolites, including quorum sensors, virulence factors, and natural products (previously referred to as secondary metabolites) (8,9). In vitro studies show that metabolites secreted by P. aeruginosa inhibit A. fumigatus filamentation and biofilm formation (10). In 2012, we detailed the metabolic interaction between these two microbes, with an emphasis on the A. fumigatus Af293-mediated biotransformation of the phenazine class of specialized metabolites produced by P. aeruginosa PA14 (11).…”

mentioning

confidence: 99%

Impact of a Transposon Insertion in phzF2 on the Specialized Metabolite Production and Interkingdom Interactions of Pseudomonas aeruginosa

et al. 2014

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fIn microbiology, gene disruption and subsequent experiments often center on phenotypic changes caused by one class of specialized metabolites (quorum sensors, virulence factors, or natural products), disregarding global downstream metabolic effects. With the recent development of mass spectrometry-based methods and technologies for microbial metabolomics investigations, it is now possible to visualize global production of diverse classes of microbial specialized metabolites simultaneously. Using imaging mass spectrometry (IMS) applied to the analysis of microbiology experiments, we can observe the effects of mutations, knockouts, insertions, and complementation on the interactive metabolome. In this study, a combination of IMS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to visualize the impact on specialized metabolite production of a transposon insertion into a Pseudomonas aeruginosa phenazine biosynthetic gene, phzF2. The disruption of phenazine biosynthesis led to broad changes in specialized metabolite production, including loss of pyoverdine production. This shift in specialized metabolite production significantly alters the metabolic outcome of an interaction with Aspergillus fumigatus by influencing triacetylfusarinine production.

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Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation

Cited by 187 publications

References 33 publications

Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists

Bacterial-Fungal Interactions: Hyphens between Agricultural, Clinical, Environmental, and Food Microbiologists

Interkingdom metabolic transformations captured by microbial imaging mass spectrometry

Impact of a Transposon Insertion in phzF2 on the Specialized Metabolite Production and Interkingdom Interactions of Pseudomonas aeruginosa

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