Pseudomonas aeruginosa: A review of their Pathogenesis and Prevalence in Clinical Settings and the Environment

Streeter, Klrissa; Katouli, Mohammad

doi:10.18869/modares.iem.2.1.25

Cited by 115 publications

(89 citation statements)

References 110 publications

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“…Analysis revealed lasB gene (89.0%) and exoS (84.0%) were the most detected in this study and this could be justified by the fact that P. aeruginosa secretes elastase (LasB); a metalloproteinase involved in the host colonization and tissue damage [5]. Previous studies have shown a high prevalence of the lasB gene in P. aeruginosa, irrespective of their isolation origin [5,6,24]. The prevalence of ExoS observed in this study shows that the strain of Pseudomonas aeruginosa of animal origin could be involved in tissue destruction; ExoS gene is involved in lung infection and may be important for bacterial dissemination [6,7,24].…”

Section: Discussionmentioning

confidence: 61%

“…This alginate prevalence indicates that isolated strains of animal products are involved in the formation of biofilms as alginates have been widely regarded as the major exopolysaccharides of the biofilm matrix [24,25]. The prevalences of plcH (71.0%) gene showed that strains isolated were able of secreting hemolytic exoenzyme and phospholipase C and thus strains could be involved in pulmonary infections [26,27].…”

Section: Discussionmentioning

confidence: 93%

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Molecular Identification and Virulence Factors of Pseudomonas Aeruginosa Strains Isolated from Animal Products

Benie

Dadié

Guessennd

et al. 2017

JBMOA

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 93%

Molecular Identification and Virulence Factors of Pseudomonas Aeruginosa Strains Isolated from Animal Products

Benie

Dadié

Guessennd

et al. 2017

JBMOA

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“…Multidrug-resistant Pseudomonas strains are disseminated worldwide 45 and may cause infections with high mortality rates among debilitated persons. 46 Environmental bacteria may be involved in opportunistic infections that may be of fatal consequence if the bacteria are antibiotic-resistant (regardless of whether they have intrinsic or acquired resistance). Remarkably, wileyonlinelibrary.com/jsfa nine of the Pseudomonas isolates from the present study were resistant to five antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

et al. 2019

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BACKGROUND Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics. RESULTS Five hundred and ninety‐five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) ≥ 16 mmol L−1 were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs ≥ 12 mmol L−1 for nickel. Ninety‐six metal‐tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera Pseudomonas (37%), Bacillus (23%) and Chryseobacterium (20%), while 6% were identified as Variovorax, 4% as Stenotrophomonas and 2% as Serratia or Burkholderia. Highest copper tolerance was detected among Pseudomonas. Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim/sulfamethoxazole. CONCLUSION Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. © 2019 Society of Chemical Industry

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“…Pseudomonas aeruginosa belongs to the Gram-negative Gammaproteobacteria class [9] and the Pseudomonadaceae family. Of all the species in the Pseudomonas genus, P. aeruginosa is the most common agent causing infections in humans [10]. It is abundant in the environment in general, and especially copious in the water and wastewater systems [10], making accidental inoculation difficult to avoid.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

Armijo¹,

Wawrzyniec²,

Kopciuch³

et al. 2020

J Nanobiotechnol

121

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Background: Novel methods are necessary to reduce morbidity and mortality of patients suffering from infections with Pseudomonas aeruginosa. Being the most common infectious species of the Pseudomonas genus, P. aeruginosa is the primary Gram-negative etiology responsible for nosocomial infections. Due to the ubiquity and high adaptability of this species, an effective universal treatment method for P. aeruginosa infection still eludes investigators, despite the extensive research in this area. Results:We report bacterial inhibition by iron-oxide (nominally magnetite) nanoparticles (NPs) alone, having a mean hydrodynamic diameter of ~ 16 nm, as well as alginate-capped iron-oxide NPs. Alginate capping increased the average hydrodynamic diameter to ~ 230 nm. We also investigated alginate-capped iron-oxide NP-drug conjugates, with a practically unchanged hydrodynamic diameter of ~ 232 nm. Susceptibility and minimum inhibitory concentration (MIC) of the NPs, NP-tobramycin conjugates, and tobramycin alone were determined in the PAO1 bacterial colonies. Investigations into susceptibility using the disk diffusion method were done after 3 days of biofilm growth and after 60 days of growth. MIC of all compounds of interest was determined after 60-days of growth, to ensure thorough establishment of biofilm colonies. Conclusions:Positive inhibition is reported for uncapped and alginate-capped iron-oxide NPs, and the corresponding MICs are presented. We report zero susceptibility to iron-oxide NPs capped with polyethylene glycol, suggesting that the capping agent plays a major role in enabling bactericidal ability in of the nanocomposite. Our findings suggest that the alginate-coated nanocomposites investigated in this study have the potential to overcome the bacterial biofilm barrier. Magnetic field application increases the action, likely via enhanced diffusion of the iron-oxide NPs and NP-drug conjugates through mucin and alginate barriers, which are characteristic of cystic-fibrosis respiratory infections. We demonstrate that iron-oxide NPs coated with alginate, as well as alginate-coated magnetite-tobramycin conjugates inhibit P. aeruginosa growth and biofilm formation in established colonies. We have also determined that susceptibility to tobramycin decreases for longer culture times. However, susceptibility to the iron-oxide NP © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

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Pseudomonas aeruginosa: A review of their Pathogenesis and Prevalence in Clinical Settings and the Environment

Cited by 115 publications

References 110 publications

Molecular Identification and Virulence Factors of Pseudomonas Aeruginosa Strains Isolated from Animal Products

Molecular Identification and Virulence Factors of Pseudomonas Aeruginosa Strains Isolated from Animal Products

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

Antibacterial activity of iron oxide, iron nitride, and tobramycin conjugated nanoparticles against Pseudomonas aeruginosa biofilms

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