Pseudomonas 2 F: Kinetics of growth and accumulation of poly - D(-)-3-hydroxybutyric acid (poly - HB)

Braunegg, Gerhart; Korneti, L.

doi:10.1007/bf00134727

Cited by 6 publications

(2 citation statements)

References 6 publications

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“…This case is described as the so-called "non-growth associated PHA production"; however, this model does not reflect the total truth; besides some PHA producers that produce high amounts of PHA already under nutritionally balanced conditions ("growth-associated PHA production"), PHA-producers with typically clearly separated phases of biomass and PHA formation can also contain significant amounts of PHA even without nutrient limitation. An extreme situation is described for so-called "PHA hyper-accumulators" such as Pseudomonas 2F, which show extraordinary high PHA accumulation rates after a period of starvation and subsequent refeeding of exogenous carbon sources [54]. This makes it clear that an optimized PHA production process needs to be in accordance with the process kinetics, the feeding regime for the different nutrient components and the process engineering.…”

Section: Kinetics Of Pha Biosynthesismentioning

confidence: 99%

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Koller

2018

Fermentation

145

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Polyhydroxyalkanoates (PHA) are microbial biopolyesters utilized as "green plastics". Their production under controlled conditions resorts to bioreactors operated in different modes. Because PHA biosynthesis constitutes a multiphase process, both feeding strategy and bioreactor operation mode need smart adaptation. Traditional PHA production setups based on batch, repeated batch, fed-batch or cyclic fed-batch processes are often limited in productivity, or display insufficient controllability of polyester composition. For highly diluted substrate streams like is the case of (agro) industrial waste streams, fed-batch enhanced by cell recycling has recently been reported as a viable tool to increase volumetric productivity. As an emerging trend, continuous fermentation processes in single-, two-and multi-stage setups are reported, which bring the kinetics of both microbial growth and PHA accumulation into agreement with process engineering and allow tailoring PHA's molecular structure. Moreover, we currently witness an increasing number of CO 2-based PHA production processes using cyanobacteria; these light-driven processes resort to photobioreactors similar to those used for microalgae cultivation and can be operated both discontinuously and continuously. This development is parallel to the emerging use of methane and syngas as abundantly available gaseous substrates, which also calls for bioreactor systems with optimized gas transfer. The review sheds light on the challenges of diverse PHA production processes in different bioreactor types and operational regimes using miscellaneous microbial production strains such as extremophilic Archaea, chemoheterotrophic eubacteria and phototrophic cyanobacteria. Particular emphasis is dedicated to the limitations and promises of different bioreactor-strain combinations and to efforts devoted to upscaling these processes to industrially relevant scales.

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Section: Kinetics Of Pha Biosynthesismentioning

confidence: 99%

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Koller

2018

Fermentation

145

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show abstract

“…which, however, this model does not reflect the total truth; beside some PHA producers which produce high amounts of PHA already under nutritionally balanced conditions ("growth-associated PHA production"), also PHA-producers with typically clearly separated phases of biomass and PHA formation can contain significant amounts of PHA even without nutrient limitation. An extreme situation is described for so-called "PHA hyper-accumulators" such as Pseudomonas 2F, which show extraordinary high PHA accumulation rates after a period of starvation and subsequent refeeding of exogenous carbon source [47]. This makes clear that an optimized PHA production process needs to bring in accordance the process kinetics, the feeding regime for the different nutrient components, and the process engineering.…”

Section: Kinetics Of Pha Biosynthesismentioning

confidence: 99%

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Koller¹

2018

Preprint

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Polyhydroxyalkanoates (PHA) are microbial biopolyesters utilized as “green plastics”. Their production under controlled conditions resorts to bioreactors operated in different modes. Because PHA biosynthesis constitutes a multiphase process, both feeding strategy and bioreactor operation mode need smart adaptation. Traditional PHA production setups based on batch, repeated batch, fed-batch or cyclic fed-batch processes are often limited in productivity, or display insufficient controllability of polyester composition. For highly diluted substrate streams like it is the case for (agro)industrial waste streams, fed-batch enhanced by cell recycling were recently reported as a viable tool to increase volumetric productivity. As emerging trend, continuous fermentation processes in single-, two-, and multi-stage setups are reported, which bring the kinetics of both microbial growth and PHA accumulation into agreement with process engineering, and allow tailoring PHA´s molecular structure. Moreover, we currently witness an increasing number of CO2-based PHA production processes using cyanobacteria; these light-driven processes resort to photobioreactors similar to those used for microalgae cultivation, and can be operated both discontinuously and continuously. This development goes in parallel to the emerging use of methane and syngas as an abundantly available gaseous substrates, which also calls for bioreactor systems with optimized gas transfer. The review sheds light on the challenges of diverse PHA production processes in different bioreactor types and operational regimes using miscellaneous microbial production strains such as extremophilic Archaea, chemoheterotrophic eubacteria and phototrophic cyanobacteria. Particular emphasize is dedicated to the limitations and promises of different bioreactor-strain combinations, and to efforts devoted to upscaling these processes to industrially relevant scales.

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Microbial Degradation of Polyesters

Jendrossek

2001

Advances in Biochemical Engineering/Biotechnology

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Pseudomonas 2 F: Kinetics of growth and accumulation of poly - D(-)-3-hydroxybutyric acid (poly - HB)

Cited by 6 publications

References 6 publications

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Microbial Degradation of Polyesters

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