Pseudokabatana alburnus n. gen. n. sp., (Microsporidia) from the liver of topmouth culter Culter alburnus (Actinopterygii, Cyprinidae) from China

Liu, X. H.; Stentiford, Grant D.; Voronin, V. N.; Sato, Hiroshi; Li, A. H.; Zhang, J. Y.

doi:10.1007/s00436-019-06303-z

Cited by 12 publications

(13 citation statements)

References 33 publications

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“…Glutaraldehyde-fixed cysts were washed three times for 10 min in sodium cacodylate buffer and then fixed with 1% osmium tetroxide (OsO4) in the same buffer for 1 h. After dehydration through a gradually ascending series of ethanol and propylene oxide, samples were embedded in Spur resin. Ultrathin sections (70-90 nm) were mounted on an uncoated copper grid and stained with uranyl acetate and lead citrate (Liu et al, 2019). Sections of two cysts were examined using a Hitachi HT-7700 transmission electron microscope (TEM).…”

Section: Transmission Electron Microscopy (Tem) Observationmentioning

confidence: 99%

“…Fish are the common hosts of aquatic microsporidia and some fish-infecting microsporidia are known to cause disastrous diseases in aquaculture (Vávra and Lukeš, 2013;Kent et al, 2014). More than 160 species belonging to about 22 genera are known to infect fishes (Liu et al, 2019). Although a few fish-infecting microsporidia have a broad host range, the majority of species show a comparative strict host specificity (Summerfelt and Goodwin, 2010;Kent et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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First report ofOvipleistophora ovariaeandO. diplostomuriin China provides new insights into the intraspecific genetic variation and extends their distribution

et al. 2021

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Microsporidia of the genus Ovipleistophora are generally parasites of fishes and aquatic crustaceans. In the current study, Ovipleistophora diplostomuri and O. ovariae were firstly reported from Culter alburnus and Xenocypris argentea and Parabramis pekinensis, respectively. Both of them exclusively infected fish ovary and were morphologically, ultrastructurally and genetically characterized. Sporogony occurred in direct contact with the host cell cytoplasm and sporophorous vesicles were not observed for the new isolates of these two Ovipleistophora species. Spores of O. ovariae were for the first time observed to be dimorphic. Genetic analysis indicated that the genetic variation in the ITS and LSU sequences was distinct among between-host O. diplostomuri isolates. High sequence variation in ITS sequence suggests that it can be a reliable molecular marker to explore the population genetics of O. diplostomuri. This is the first report of these two Ovipleistophora species in China which extends their host and geographical range.

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Section: Transmission Electron Microscopy (Tem) Observationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First report ofOvipleistophora ovariaeandO. diplostomuriin China provides new insights into the intraspecific genetic variation and extends their distribution

et al. 2021

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“…Microsporidia are characterized by the production of resistant spores possessing the evaginable polar tube which transmit the infective sporoplasm to the host cytoplasm or nucleoplasm during the spore germination ( Dussaubat et al, 2013 ; Roudel et al, 2013 ; Vavra and Lukes, 2013 ). Over 1,600 microsporidia belonging to about 200 genera have been described worldwide, among which more than 160 species falling within 22 genera are known to infect fishes ( Liu et al, 2019 ). Some fish-infecting microsporidians are economically important pathogens for wild and aquaculture fish, such as Glugea pagri infecting the red sea bream Pagrus major ( Su et al, 2014 ), Enterospora epienpheli infecting the juvenile groupers Epinephelus spp.…”

Section: Introductionmentioning

confidence: 99%

Intraspecific genetic diversity of the fish-infecting microsporidian parasite Pseudokabatana alburnus (Microsporidia)

Weng

Zhang²,

Xin³

et al. 2023

Front. Microbiol.

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Pseudokabatana alburnus is a xenoma-forming fish microsporidium, firstly described from the liver of the Culter alburnus from Poyang Lake in China. In the present study, P. alburnus was firstly reported from the ovary of 6 other East Asian minnows, including Squaliobarbus curriculus, Hemiculter leucisculus, Cultrichthys erythropterus, Pseudolaubuca engraulis, Toxabramis swinhonis, and Elopichthys bambusa. Genetic analysis revealed high sequence diversity in the ribosomal internal transcribed spacer region (ITS) and the largest subunit of RNA polymerase II (Rpb1) loci of P. alburnus isolated from different hosts and locations. The variation of Rpb1 mainly occurred in the 1,477–1737 bp regions. The presence of a wide variety of Rpb1 haplotypes within a single fish host, together with evidence of genetic recombination suggested that P. alburnus may have the intergenomic variation and sexual reproduction might be present in other hosts (possibly freshwater shrimp). Phylogenetic analysis and population genetic analysis showed that there was no geographical population divergence for P. alburnus. Homogeneity and high variability of ITS sequences indicates that ITS may be a suitable molecular marker to distinguish different P. alburnus isolates. Our data confirm the broad geographical distribution and host range of P. alburnus in the middle and lower reaches of the Yangtze River. Additionally, we emendated the genus Pseudokabatana to exclude the infection site, liver as one of the taxonomic criteria, and proposed that fish ovary was be the general infection site of P. alburnus.

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“…Presently, over 1600 species belonging to 200 or more recognized genera have been described around the world (Stentiford et al, 2013a). Among them, almost half were recorded as infecting aquatic organisms, including fish, shrimps, crabs, zooplankton, and zoobenthos (Liu et al, 2019; Stentiford & Dunn, 2014; Stentiford et al, 2013a). In China, research about microsporidia has mainly focused on Terresporidia, while Aquasporidia species have received little attention (Liu et al, 2019).…”

mentioning

confidence: 99%

“…Among them, almost half were recorded as infecting aquatic organisms, including fish, shrimps, crabs, zooplankton, and zoobenthos (Liu et al, 2019; Stentiford & Dunn, 2014; Stentiford et al, 2013a). In China, research about microsporidia has mainly focused on Terresporidia, while Aquasporidia species have received little attention (Liu et al, 2019). In fact, cases of microsporidiosis have been reported frequently in aquacultural animals in China in recent years, causing huge economic losses.…”

mentioning

confidence: 99%

New isolate of Loma psittaca (Microsporidia: Glugeidae) infecting the stomach wall of cultured hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) in South China

Liu

et al. 2021

J Eukaryotic Microbiology

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Loma psittaca, previously described as inhabiting the intestinal mucosa of an anadromous fish, Colomesus pisttacus, from the Amazon Basin, is reported as being found for the first time in a marine fish, the hybrid grouper (Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀), from Lingshui city, Hainan Province, China, expanding the geographical distribution and host range of this parasite. Numerous whitish xenomas (0.5-0.7 mm in diameter) of this new isolate of L. psittaca were found distinctly in the muscle layer of the host stomach wall. Electron microscopic observations showed a monokaryotic nucleus in all developmental stages. Round or elongated multinucleate merogonial plasmodia surrounded by numerous mitochondria were observed initially, subsequently transforming into uninucleate sporonts through multiple fissions.Sporonts, each with a large centrally positioned nucleus, further developed into sporoblasts. Each sporoblast mother cell gave rise to two uninucleate sporoblasts by binary fission. Mature spores were ellipsoidal, measuring 4.0 ± 0.3 (3.7-4.3) μm in length and 2.2 ± 0.2 (2.1-2.5) μm in width. Spores possessed a mushroom-like anchoring disk, a bipartite polarplast, isofilar polar filaments arranged in 12-14 turns in one row, and a trilaminar spore wall. The obtained partial SSU rRNA gene sequence of the new isolate was 1330 bp in length and showed 99.4% sequence similarity with an estuary isolate of L. psittaca previously reported in South America. SSU rRNA gene-based phylogenetic analyses demonstrated that the two L. psittaca isolates first clustered together and then formed a dichotomy that included the digestive-tract-infecting Loma species, L. acerinae, with high support values within group I.

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Pseudokabatana alburnus n. gen. n. sp., (Microsporidia) from the liver of topmouth culter Culter alburnus (Actinopterygii, Cyprinidae) from China

Cited by 12 publications

References 33 publications

First report ofOvipleistophora ovariaeandO. diplostomuriin China provides new insights into the intraspecific genetic variation and extends their distribution

First report ofOvipleistophora ovariaeandO. diplostomuriin China provides new insights into the intraspecific genetic variation and extends their distribution

Intraspecific genetic diversity of the fish-infecting microsporidian parasite Pseudokabatana alburnus (Microsporidia)

New isolate of Loma psittaca (Microsporidia: Glugeidae) infecting the stomach wall of cultured hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) in South China

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