Pseudognaphalium affine Extract Alleviates COPD by Inhibiting the Inflammatory Response via Downregulation of NF-κB

Abstract

Chronic obstructive pulmonary disease (COPD) is a chronic respiratory disease with limited therapeutic options. Pseudognaphalium affine (D. Don) Anderb. is a medicinal and edible plant used to treat cough, asthma, and COPD for a long time in folk medicine. The objective of this study is to evaluate the effect of Pseudognaphalium affine (D. Don) Anderb. extract (GAE) and investigate the possible underlying mechanism in vivo and in vitro. In vivo, the administration of GAE in a rat COPD model could significantly ameliorate lung damage and pulmonary function by inhibiting the production of pro-inflammatory cytokines. Western blot and real-time PCR results showed that GAE could suppress nuclear translocation of nuclear factor-kappa B (NF-κB), which indicated that GAE down-regulated the NF-κB pathway. Moreover, GAE protected against tumor necrosis factor (TNF)-α induced inflammatory response in BEAS-2B and inhibited the NF-κB pathway. All data suggested that GAE exhibited its anti-COPD effect by inhibiting pro-inflammatory cytokines, which may be associated with the inhibition of the NF-κB pathway.

Suppression of long noncoding RNA SNHG6 alleviates cigarette smoke‐induced lung inflammation by modulating NF‐κB signaling

“…We also evaluated NF-κB signaling, as measured by the secretion of TNF-α, IL-1β, and IL-6, because NF-κB activation is closely related to CS-induced inflammation. 1,28 We found that CSE treatment increased the expression of p-NF-κB p65 and promoted NF-κB p65 nuclear localization. Furthermore, SNHG6 overexpression enhanced CSE-induced inflammation, whereas blockage of NF-κB signaling by BAY11-7082 significantly reduced this effect, suggesting that the enhancement of CS-induced inflammation by SNHG6 was dependent on NF-κB signaling.…”

“…We also evaluated NF‐κB signaling, as measured by the secretion of TNF‐α, IL‐1β, and IL‐6, because NF‐κB activation is closely related to CS‐induced inflammation 1,28 . We found that CSE treatment increased the expression of p‐NF‐κB p65 and promoted NF‐κB p65 nuclear localization.…”

Effects of Ipratropium Bromide Combined with Traditional Chinese Medicine Intervention on the Pulmonary Function and Psychological Status of Patients with Chronic Obstructive Pulmonary Disease

“…Traditional Chinese medicine has unique advantages in the treatment of COPD. Clinical practice has confrmed that traditional Chinese medicine nursing has a good efect on relieving symptoms, such as shortness of breath and dyspnea, in patients with chronic respiratory diseases [32,[43][44][45][46][47][48][49]. In this study, patients with COPD were treated with TCM nursing measures, such as emotional nursing, exercise intervention, and acupoint massage, in accordance with the principles of syndrome diferentiation and nursing.…”