Pseudocholinesterase Activity and Its Origin in Human Oral Fluid

Ryhänen, Reijo; Närhi, Matti; Puhakainen, E.; Hänninen, Osmo; Kontturi-Närhi, Vuokko

doi:10.1177/00220345830620010501

Cited by 19 publications

(15 citation statements)

References 12 publications

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“…In this study, it was seen that salivary PChE activity was increased in patients with AD compared to the control group. Ryhaneh's result showed no relation of PChE activities in saliva and serum samples . Therefore, the level of enzymes in saliva may be influenced by many local factors and does not show serum level in this disease.…”

Section: Discussionmentioning

confidence: 90%

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Evaluation of salivary acetylcholinesterase and pseudocholinesterase in patients with Alzheimer's disease: A case–control study

Ahmadi‐Motamayel

Goodarzi

Tarazi

et al. 2018

Special Care in Dentistry

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Aim:The aim of this study was to evaluate acetylcholinesterase (AChE) and pseudocholinesterase (PChE) in whole saliva in patients with Alzheimer's disease (AD) and in healthy subjects. Saliva has a high potential for keeping track of general health and diseases. AD is a type of dementia with reduction in brain cholinergic markers that causes memory, thinking, and behavior problems. Up to 90% decrease in AChE activity has been observed in AD. Methods and results:Thirty healthy subjects and 30 patients with AD participated in this study. Saliva samples were collected from 8 to 10 AM. AChE and PChE of saliva were assessed by the Ellman method. Statistical comparison was performed using SPSS 16 for t-test. The activity of AChE and PChE significantly increased in the group with AD compared to the healthy subjects. Sex had no effect on the activities of these enzymes. No correlation existed between the duration of illness and enzymatic activity. The enzyme levels reduced with age.Conclusion: AChE and PChE levels were increased in saliva samples of patients with AD. Therefore, saliva has the potential for being used for the purpose of biomarker evaluation to replace cerebrospinal fluid in patients with AD.

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Section: Discussionmentioning

confidence: 90%

“…The color developed from the reaction of enzyme products with DTNB was measured at 412 nm. The Bradford method was used to evaluate saliva protein level …”

Section: Methodsmentioning

confidence: 99%

Evaluation of salivary acetylcholinesterase and pseudocholinesterase in patients with Alzheimer's disease: A case–control study

Ahmadi‐Motamayel

Goodarzi

Tarazi

et al. 2018

Special Care in Dentistry

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“…In our study, mean CVs between individuals for the pipette and Salivette ® samples, respectively, were 66 and 77%. Other authors have reported activity equating to interindividual CVs of between 4 and 89% (Abdollahi et al, 1995(Abdollahi et al, , 1996Ryhanen et al, 1983;Ryhanen, 1983;Sayer et al, 2004b;Ueda & Yamaguchi, 1976;Yamalik et al, 1990Yamalik et al, , 1991.…”

Section: Discussionmentioning

confidence: 94%

“…Low levels of cholinesterase activity are also known to be present in human saliva (Abdollahi et al, 1995(Abdollahi et al, , 1996Chauncey et al, 1954;Drjankova & Zaprjanova, 1981;Giddon & Lisanti, 1962;Ryhanen et al, 1983;Ryhanen, 1983;Sayer et al, 2004Sayer et al, , 2004bUeda & Yamaguchi, 1976;Yamalik et al, 1990Yamalik et al, , 1991. In a group of exposed pesticide factory workers, cholinesterase activity in saliva was lower than the activity in healthy controls (Abdollahi et al, 1996).…”

mentioning

confidence: 99%

Measuring Cholinesterase Activity in Human Saliva

Henn

McMaster

Padilla

2006

Journal of Toxicology and Environmental Health, Part A

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To assess the potential for using saliva in pesticide biomonitoring, the consistency of cholinesterase activity in human saliva collected over time was examined. In this pilot study, saliva was collected from 20 healthy adults once per week for 5 consecutive weeks using 2 different collection methods: a disposable plastic pipette, and a cotton-wool roll. A brief questionnaire was conducted each week to document changes in exposure to cholinesterase inhibitors for the duration of the sampling. To measure cholinesterase activity, an existing radiometric method was modified to make it suitable for human saliva. Using this method, cholinesterase activity was measurable in saliva, and duplicate samples showed reliable repeatability. Activity in both collection methods ranged from 3 to 265 nmol/h/ml saliva (mean = 52 +/- 37 [SD] nmol/h/ml saliva). For some individuals, enzyme activity was consistent over the five sampling weeks; for others, activity was highly variable. Coefficients of variation (CVs) were calculated to assess variability, and mean CVs were the same for both collection methods (about 35%). Adjusting for protein concentration in the pipette-collected samples did not change results. Both collection methods worked well for collecting between 1 and 3 ml saliva, but at the majority of visits (86%), participants preferred the cotton-wool roll. Results from this study suggest that saliva may be a useful indicator of potential neurotoxic effects from exposure to organophosphorus and carbamate pesticides, but that factors affecting variability should be explored further.

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“…Human cholinesterase consists of acetylcholinesterase and pseudocholinesterase 40) . Acetylcholinesterase was used in this study to investigate the biodegradation of resin at the interfaces.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of all-in-one self-etch adhesive systems at the resin-dentin interface

et al. 2011

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This study evaluated the effects of two exogenous enzymes on the resin-dentin interface. Collagenase (Col) and acetylcholinesterase (Ach) were used to simulate salivary enzymes and accelerate the aging process of the bonding interfaces. Four adhesives, Adper Single Bond 2 (SB), Clearfil SE Bond (SE), Clearfil tri-S Bond (S3) and G-Bond (G), were bonded to the dentin surfaces. After storage in water with collagenase or acetylcholinesterase, the specimens were examined using a microtensile bond strength test (MTBS). Nanoleakage patterns were observed with a scanning electron microscope (SEM). The MTBS results demonstrated significantly lower bond strengths in the groups stored with either enzyme than in water. SB exhibited severe degradation after exposure to collagenase, while G showed severe degradation after exposure to acetylcholinesterase. All of the self-etch systems (SE, S3 and G) exhibited water-tree patterns within the adhesive layer when immersed in water for three months. The etch-and-rinse system (SB) showed nanoleakage within the hybrid layer and the adhesive.

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Pseudocholinesterase Activity and Its Origin in Human Oral Fluid

Cited by 19 publications

References 12 publications

Evaluation of salivary acetylcholinesterase and pseudocholinesterase in patients with Alzheimer's disease: A case–control study

Evaluation of salivary acetylcholinesterase and pseudocholinesterase in patients with Alzheimer's disease: A case–control study

Measuring Cholinesterase Activity in Human Saliva

Biodegradation of all-in-one self-etch adhesive systems at the resin-dentin interface

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