Pseudo-outbreak of Burkholderia cepacia blood stream infections in intensive care units of a super-speciality hospital: A cross-sectional study

Bhatia, Mohit; Loomba, Poonam; Mishra, Bibhabati; Dogra, Vinita

doi:10.5455/ijmsph.2017.0305723032017

Cited by 2 publications

(1 citation statement)

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“…Moreover, several studies have reported B. cepacia outbreaks in intensive care units (ICUs) due to contaminated water. Such as in Indian hospitals, environmental sampling in ICUs showed that water was implicated as a major environmental source (Bhatia et al, 2017;Rastogi et al, 2019). In the present study, we took one year to find the contamination source.…”

Section: Discussionmentioning

confidence: 99%

An intermittent outbreak of Burkholderia cepacia contaminating hematopoietic stem cells resulting in infusate-related blood stream infections

Raddaoui

Tanfous

Chebbi

et al. 2022

Journal of Infection Prevention

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Microbial contamination of hematopoietic stem cells (HSC), used for autologous and allogenic transplantations, is rare but could cause serious blood stream infection in transplanted patients. These infections occur immediately, or later following the formation of biofilm on the catheter lumen. The present study describes an intermittent B. cepacia HSC contamination associated with nosocomial bacteremia: from October 2011 to April 2015, 17 B. cepacia strains were isolated in HSC bags ( n = 14) and blood cultures ( n = 3) in patients hospitalized in the National Bone Marrow Transplant Center. Two epidemiologic investigations in the National Blood Transfusion Center, allowing the isolation of three strains in hygiene samples, and four interventions in this institution were done. To identify the source of this contamination, a molecular investigation was done on 23 B. cepacia strains isolated in our center from 2007 to 2015. PFGE analysis revealed five clusters. The major cluster included 18 strains isolated from HSC bags ( n = 14), blood culture ( n = 1), and water cans and bath ( n = 3). The second cluster (B) including only two and the remaining clusters (C, D, and E) contained single strains isolated before the epidemic period. These findings confirmed that the origin of the outbreak was the contaminated water used in the water bath during the thawing step of HSC bags. Based on this result, new sterile water was used for every defrosting, but HSC bags contamination persisted. In May 2015, the water bath was replaced with a dry bath and no B. cepacia strain was isolated from that date to April 2020.

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Section: Discussionmentioning

confidence: 99%