Pseudaminobacter arsenicus sp. nov., an arsenic-resistant bacterium isolated from arsenic-rich aquifers

Mu, Yao; Zhou, Xing Kui; Liu, Lan; Zhou, Xing-Kui; Zeng, Xian‐Chun; Li, Wenjun

doi:10.1099/ijsem.0.003238

Cited by 21 publications

(7 citation statements)

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“…For Cluster IV, Mesorhizobium ephedrae 6GN30 T was isolated from root endosphere of Ephedra przewalskii and lacks nod and nif genes [ 17 ], and we propose Kumtagia as the genus name. For Subcluster V-2, Pseudaminobacter arsenicus CB3 T was isolated from arsenic-rich aquifers [ 29 ], and we propose Borborobacter as the genus name. For Subcluster VII-2, Aquamicrobium aerolatum DSM 21857 T was isolated from air sampled in a duck shed [ 22 ], thus we propose Aerobium as the genus name.…”

Section: Resultsmentioning

confidence: 99%

“…The genus Pseudaminobacter was established in 1999 [ 28 ]. It comprises five validly published species: P. arsenicus [ 29 ], P. defluvii [ 28 ], P. granuli [ 30 ], P. manganicus [ 31 ], and P. salicylatoxidans [ 28 ] and a not validly named species “ P. soli " [ 32 ]. They were isolated from various aquatic environments e.g.…”

Section: Introductionmentioning

confidence: 99%

“…They were isolated from various aquatic environments e.g. arsenic-rich aquifers [ 29 ], sludge [ 28 ], wastewater treatment plant [ 30 ], and river [ 28 ]. The genus Aminobacter was established in 1992 [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Phylogenomic analyses and reclassification of the Mesorhizobium complex: proposal for 9 novel genera and reclassification of 15 species

Li,

Guo,

Sun

et al. 2024

BMC Genomics

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Backgroud The genus Mesorhizobium is shown by phylogenomics to be paraphyletic and forms part of a complex that includes the genera Aminobacter, Aquamicrobium, Pseudaminobacter and Tianweitania. The relationships for type strains belong to these genera need to be carefully re-evaluated. Results The relationships of Mesorhizobium complex are evaluated based on phylogenomic analyses and overall genome relatedness indices (OGRIs) of 61 type strains. According to the maximum likelihood phylogenetic tree based on concatenated sequences of 539 core proteins and the tree constructed using the bac120 bacterial marker set from Genome Taxonomy Database, 65 type strains were grouped into 9 clusters. Moreover, 10 subclusters were identified based on the OGRIs including average nucleotide identity (ANI), average amino acid identity (AAI) and core-proteome average amino acid identity (cAAI), with AAI and cAAI showing a clear intra- and inter-(sub)cluster gaps of 77.40–80.91% and 83.98–86.16%, respectively. Combined with the phylogenetic trees and OGRIs, the type strains were reclassified into 15 genera. This list includes five defined genera Mesorhizobium, Aquamicrobium, Pseudaminobacter, Aminobacterand Tianweitania, among which 40/41 Mesorhizobium species and one Aminobacter species are canonical legume microsymbionts. The other nine (sub)clusters are classified as novel genera. Cluster III, comprising symbiotic M. alhagi and M. camelthorni, is classified as Allomesorhizobium gen. nov. Cluster VI harbored a single symbiotic species M. albiziae and is classified as Neomesorhizobium gen. nov. The remaining seven non-symbiotic members were proposed as: Neoaquamicrobium gen. nov., Manganibacter gen. nov., Ollibium gen. nov., Terribium gen. nov., Kumtagia gen. nov., Borborobacter gen. nov., Aerobium gen. nov.. Furthermore, the genus Corticibacterium is restored and two species in Subcluster IX-1 are reclassified as the member of this genus. Conclusion The Mesorhizobium complex are classified into 15 genera based on phylogenomic analyses and OGRIs of 65 type strains. This study resolved previously non-monophyletic genera in the Mesorhizobium complex.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenomic analyses and reclassification of the Mesorhizobium complex: proposal for 9 novel genera and reclassification of 15 species

Li,

Guo,

Sun

et al. 2024

BMC Genomics

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“…The arsenic-resistant strain Ps. arsenicus CB3 T (pH range for growth 6.0–9.0) was isolated from arsenic-rich aquifers of the Jianghan Plain (Hubei, China), the groundwater of which is severely contaminated by arsenic [ 37 ]. The arsenical pump-driving ATPase ArsA involved in the removal of arsenate, antimonite, and arsenate from the cell showed the highest similarity to that of Acetobacteraceae bacterium SCN 69-10 (85.4% a. a. identity, 100% coverage).…”

Section: Resultsmentioning

confidence: 99%

Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates

Panyushkina

Булаев

Белый³

2021

Microorganisms

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Acidiphilium multivorum LMS is an acidophile isolated from industrial bioreactors during the processing of the gold-bearing pyrite-arsenopyrite concentrate at 38–42 °C. Most strains of this species are obligate organoheterotrophs that do not use ferrous iron or reduced sulfur compounds as energy sources. However, the LMS strain was identified as one of the predominant sulfur oxidizers in acidophilic microbial consortia. In addition to efficient growth under strictly heterotrophic conditions, the LMS strain proved to be an active sulfur oxidizer both in the presence or absence of organic compounds. Interestingly, Ac. multivorum LMS was able to succeed more common sulfur oxidizers in microbial populations, which indicated a previously underestimated role of this bacterium in industrial bioleaching operations. In this study, the first draft genome of the sulfur-oxidizing Ac. multivorum was sequenced and annotated. Based on the functional genome characterization, sulfur metabolism pathways were reconstructed. The LMS strain possessed a complicated multi-enzyme system to oxidize elemental sulfur, thiosulfate, sulfide, and sulfite to sulfate as the final product. Altogether, the phenotypic description and genome analysis unraveled a crucial role of Ac. multivorum in some biomining processes and revealed unique strain-specific characteristics, including the ars genes conferring arsenic resistance, which are similar to those of phylogenetically distinct microorganisms.

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“…Following four rounds of enrichment, bacterial strains were isolated from the cultures using the Hungate Rolling Tube Technique under anaerobic conditions [14]. To promote the isolation of arsenic-resistant strains, 75 mg/L of arsenic was added to the culture medium [43,44].…”

Section: Obtaining Of a Novel Arsenic-resistant Bacterium With Mn-oxi...mentioning

confidence: 99%

A Novel Mn- and Fe-Oxides-Reducing Bacterium with High Activity to Drive Mobilization and Release of Arsenic from Soils

Xiong

et al. 2023

Water

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Since Mn, Fe and As contaminants often coexist in the environment, we hypothesize that the presence of multifunctional bacteria is capable of reducing Mn and Fe oxides and promoting the mobilization and release of arsenic. However, such bacteria have not been reported yet; moreover, the impact of bacteria with the ability to simultaneously reduce Mn and Fe oxides on the formation of high-arsenic groundwater remains unclear. This study aims to address this question. Here, we found that the microbial community in the soils was able to efficiently reduce Mn oxides into Mn(II). An analysis of the microbial community structures of the soil shows that it contained Proteobacteria (41.1%), Acidobacteria (10.9%), Actinobacteria (9.5%) and other less abundant bacteria. Based on this observation, we successfully isolated a novel bacterium Cellulomonas sp. CM1, which possesses both Mn- and Fe-oxide-reducing activities. Under anaerobic conditions, strain CM1 can reduce Mn oxides, resulting in the production of 13 mg/L of Mn(II) within a span of 10 days. Simultaneously, it can reduce Fe oxides, leading to the generation of 9 mg/L of Fe(II) within 9 days when a yeast extract is used as an electron donor. During these reduction reactions, the cells were grown into a density of OD600 0.16 and 0.09, respectively, suggesting that Mn(IV) is more beneficial for the bacterial growth than Fe(III). Arsenic release assays indicate that after 108 days of anoxic incubation, approximately 126.2, 103.2 and 81.5 μg/L As(V) were mobilized and released from three soil samples, respectively, suggesting that CM1 plays significant roles in driving mobilization of arsenic from soils. These findings shed new light on the microbial processes that lead to the generation of arsenic-contaminated groundwater.

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Pseudaminobacter arsenicus sp. nov., an arsenic-resistant bacterium isolated from arsenic-rich aquifers

Cited by 21 publications

References 21 publications

Phylogenomic analyses and reclassification of the Mesorhizobium complex: proposal for 9 novel genera and reclassification of 15 species

Phylogenomic analyses and reclassification of the Mesorhizobium complex: proposal for 9 novel genera and reclassification of 15 species

Unraveling the Central Role of Sulfur-Oxidizing Acidiphilium multivorum LMS in Industrial Bioprocessing of Gold-Bearing Sulfide Concentrates

A Novel Mn- and Fe-Oxides-Reducing Bacterium with High Activity to Drive Mobilization and Release of Arsenic from Soils

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