PS212. Human Adult Stem Cells Restore Endothelial Migratory Dysfunction in a Hypoxic Environment

Abstract

nerve action potential (SNAP) amplitude, and nerve conduction velocity (NCV)). These results were correlated with peroneus muscle and peroneal nerve histology.Results: Baseline physiologic characteristics were similar between groups. Neuromuscular recovery in groups with early restoration of flow (Control, 1HR, 3HR) was similar and nearly complete (92%, 98% and 88% respectively; p Ͼ0.45). While recovery was diminished in both 6HR and Ligation; Ligation, rather than repair, exhibited greater recovery (68% vs 53%; p Ͻ0.05). These relationships correlated with the pathologic grade of degeneration, necrosis, and fibrosis (p Ͻ0.05). Using the PMR, the ischemic threshold of the extremity is reached at 5 hours.Conclusions: This study reports a novel and translatable animal model of extremity ischemia and reperfusion correlating ischemic time to functional markers of recovery. In this model an ischemic threshold of 5 hours is defined after which Ligation is associated with less irreversible injury than surgical restoration of flow.