PS-b-PAA nanovesicles coated by modified PEIs bearing hydrophobic and hydrophilic groups

Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression

“…The derivatization of BPEI with sugar residues was hypothesized to be a clever strategy to reduce the charge density in the chains, consequently reducing cytotoxicity. The BPEI modification was performed via reductive amination with lactose . The final material was characterized by 1 HNMR and FTIR.…”

“…BPEI was chemically modified with lactose (Lac) according to previously reported procedures . Briefly, BPEI (0.30 g; 7.0 mmol per monomer) was dissolved in 40 mL of sodium borate (0.10 mol L −1 ) in aqueous solution for 24 h. Lactose monohydrate (1.26 g; 3.5 mmol) was dissolved in 10 mL of sodium borate solution and added to the BPEI solution.…”

Polystyrene-b-poly (acrylic acid) nanovesicles coated by modified chitosans for encapsulation of minoxidil

“…These interactions depend mainly on the particle surface charge, allowing these dynamic changes to be measured by zeta potential and thereby allowing us to infer the occurrence of interactions by the change on the surface charge when these charged particles form complexes with polyelectrolytes. This measurement informs the electrostatic repulsion and the stability of such suspensions (Bellettini et al, 2015). In addition, these changes can also be estimated from DLS measurements and observed by TEM images.…”

“…protonation of the poly (acrylic acid) (PAA) block in aqueous solution (Discher, Eisenberg, 2002;Bellettini et al, 2015;Dong, Lindau, Ober, 2009). The use of copolymers containing ionizable groups enables the coating of these nanostructures by electrostatic adsorption of opposite-charged polyelectrolytes (Burke, Eisenberg, 2001;Choucair, Lavigueur, Eisenberg, 2004) aiming at improving biocompatibility (Bellettini et al, 2015), permeability (Caon et al, 2014) or even the selective targeting through recognition by specific receptors, for example.…”

“…In this sense, copolymers represent a class of macromolecules that are perfectly suitable to prepare nanocarriers due to their ability to self-assemble into well-organized structures. This behavior is due to the incompatibility between the blocks, which, when exposed to mixed selective solvents, generate well-defined macromolecular structures often with distinct hydrophilic and hydrophobic domains (Discher, Eisenberg, 2002;Bellettini et al, 2015), thus allowing the incorporation of drugs of different solubilities (Zhang, Eisenberg, 1995). Despite this, the majority of the studies carried out so far aims at the incorporation of hydrophobic drugs, and the use of hydrophilic drugs is little explored (Bastakoti et al, 2013;Caon et al, 2014).…”