Prussian Blue Staining to Visualize Iron Oxide Nanoparticles

Bitonto, Valeria; Garello, Francesca; Scherberich, Arnaud; Filippi, Miriam

doi:10.1007/978-1-0716-2675-7_26

Cited by 7 publications

(5 citation statements)

References 31 publications

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“…Perls stain, also known as the hemin stain, is a very classic histochemical reaction and a sensitive and traditional method for displaying trivalent iron in tissues. The principle of the staining process is that potassium ferrocyanide solution separates trivalent iron ions from proteins with dilute hydrochloric acid, and the trivalent iron reacts with potassium ferrocyanide to form an insoluble blue compound, namely trivalent iron ferrocyanide, also known as Prussian blue [ 25 ]. Paraffin-embedded slices experienced dewaxing and rehydration as section 2.8 and were subjected to Perls staining in the dark using a Prussian blue staining kit.…”

Section: Methodsmentioning

confidence: 99%

Orexin-A alleviates ferroptosis by activating the Nrf2/HO-1 signaling pathway in traumatic brain injury

Kang,

Ren,

Huang

et al. 2024

Aging

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Background: Traumatic Brain Injury (TBI) has high disability and mortality rate. Oxidative stress and ferroptosis are important pathophysiological characteristics after TBI. Orexin-A (OXA) can alleviate neuronal damage in diverse neurological disorders. Nevertheless, the role and mechanism of OXA in TBI stay unknown. Objectives: The research investigated protection influence of OXA on TBI and its potential mechanisms. Methods: Male Sprague-Dawley rats were randomly grouped into: sham, TBI, TBI + normal saline (NS) and TBI+OXA groups. TBI model was constructed in rat via modified Feeney’s approach, and OXA treatment was administered following construction of TBI model. Results: Relative to TBI+NS group, TBI+OXA group displayed greatly recovered tissue damage and neurological deficits. Additionally, OXA eased oxidative stress as well as ferroptosis in cerebral cortex of rats following TBI. Furthermore, OXA increased Nrf2 expression and regulating factors HO-1 and NQO1 in cerebral cortex of TBI rats. Conclusions: Our research found OXA may restrain ferroptosis via Nrf2/HO-1 signaling pathway activation, thereby reducing brain injury after TBI.

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Section: Methodsmentioning

confidence: 99%

Orexin-A alleviates ferroptosis by activating the Nrf2/HO-1 signaling pathway in traumatic brain injury

Kang,

Ren,

Huang

et al. 2024

Aging

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“…Ethanol residues were removed from the wells and the samples were mounted for later visualization. [ 49 ]…”

Section: Methodsmentioning

confidence: 99%

Targeted Rapamycin Delivery via Magnetic Nanoparticles to Address Stenosis in a 3D Bioprinted in Vitro Model of Pulmonary Veins

Ning,

Zanella,

Tomov

et al. 2024

Advanced Science

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Vascular cell overgrowth and lumen size reduction in pulmonary vein stenosis (PVS) can result in elevated PV pressure, pulmonary hypertension, cardiac failure, and death. Administration of chemotherapies such as rapamycin have shown promise by inhibiting the vascular cell proliferation; yet clinical success is limited due to complications such as restenosis and off‐target effects. The lack of in vitro models to recapitulate the complex pathophysiology of PVS has hindered the identification of disease mechanisms and therapies. This study integrated 3D bioprinting, functional nanoparticles, and perfusion bioreactors to develop a novel in vitro model of PVS. Bioprinted bifurcated PV constructs are seeded with endothelial cells (ECs) and perfused, demonstrating the formation of a uniform and viable endothelium. Computational modeling identified the bifurcation point at high risk of EC overgrowth. Application of an external magnetic field enabled targeting of the rapamycin‐loaded superparamagnetic iron oxide nanoparticles at the bifurcation site, leading to a significant reduction in EC proliferation with no adverse side effects. These results establish a 3D bioprinted in vitro model to study PV homeostasis and diseases, offering the potential for increased throughput, tunability, and patient specificity, to test new or more effective therapies for PVS and other vascular diseases.

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“…The Prussian blue histological staining method was utilized to detect intracellular iron concentrations [90,91]. RAW 264.7 cells in RPMI (1.5 mL) were plated into a cover glass at 3 × 10 5 cells/well in 6-well plates.…”

Section: Prussian Blue Staining For Intracellular Iron Detectionmentioning

confidence: 99%

Novel Fe3O4 Nanoparticles with Bioactive Glass–Naproxen Coating: Synthesis, Characterization, and In Vitro Evaluation of Bioactivity

Valverde,

dos Santos,

Viana

et al. 2024

IJMS

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Immune response to biomaterials, which is intimately related to their surface properties, can produce chronic inflammation and fibrosis, leading to implant failure. This study investigated the development of magnetic nanoparticles coated with silica and incorporating the anti-inflammatory drug naproxen, aimed at multifunctional biomedical applications. The synthesized nanoparticles were characterized using various techniques that confirmed the presence of magnetite and the formation of a silica-rich bioactive glass (BG) layer. In vitro studies demonstrated that the nanoparticles exhibited bioactive properties, forming an apatite surface layer when immersed in simulated body fluid, and biocompatibility with bone cells, with good viability and alkaline phosphatase activity. Naproxen, either free or encapsulated, reduced nitric oxide production, an inflammatory marker, while the BG coating alone did not show anti-inflammatory effects in this study. Overall, the magnetic nanoparticles coated with BG and naproxen showed promise for biomedical applications, especially anti-inflammatory activity in macrophages and in the bone field, due to their biocompatibility, bioactivity, and osteogenic potential.

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Prussian Blue Staining to Visualize Iron Oxide Nanoparticles

Cited by 7 publications

References 31 publications

Orexin-A alleviates ferroptosis by activating the Nrf2/HO-1 signaling pathway in traumatic brain injury

Orexin-A alleviates ferroptosis by activating the Nrf2/HO-1 signaling pathway in traumatic brain injury

Targeted Rapamycin Delivery via Magnetic Nanoparticles to Address Stenosis in a 3D Bioprinted in Vitro Model of Pulmonary Veins

Novel Fe3O4 Nanoparticles with Bioactive Glass–Naproxen Coating: Synthesis, Characterization, and In Vitro Evaluation of Bioactivity

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