Prunus mume extract exhibits antimicrobial activity against pathogenic oral bacteria

Seneviratne, Chamida J.; Wong, Ricky W.K.; Hägg, Urban; Chen, Yong; Herath, Thanuja D.; Samaranayake, P. Lakshman; Kao, Ryt

doi:10.1111/j.1365-263x.2011.01123.x

Cited by 32 publications

(28 citation statements)

References 16 publications

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“…3) Since an HPLC analysis revealed that the main compounds of P. mume are organic acids, citric acid and other organic acids were suggested to be predominantly involved in the oral antimicrobial effects. However, Seneviratne et al also suggested that other active components in the P. mume extract also have considerable antibacterial activity, and for clinical application, more molecular studies are needed to elucidate the mechanism and the identity of the other active components involved in the antimicrobial activity of P. mume.…”

Section: Involvement Of Citric Acid In Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial Activity of the Phenolic Compounds of Prunus mume against Enterobacteria

Mitani

Ota

Inaba

et al. 2018

Biological & Pharmaceutical Bulletin

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Mume fruit, the Japanese apricot (Prunus mume SIEB. et ZUCC.), is popular in Japan and is mostly consumed in the pickled form called umeboshi. This fruit is known to have anti-microbial properties, but the principal constituents responsible for the antimicrobial properties have not yet been elucidated. We investigated the antimicrobial activities of the phenolic compounds in P. mume against enterobacteria. In this study, growth inhibitory activities were measured as an index of the antibacterial activities. The phenolic compounds were prepared from a byproduct of umeboshi called umesu or umezu (often translated as "mume vinegar"). Umesu or umezu phenolics (UP) contain approximately 20% phenolic compounds with p-coumaric acid as a standard and do not contain citric acid. We observed the inhibitory effects of UP against the growth of some enterobacteria, at a relatively high concentration (1250-5000 µg/mL). Alkali hydrolysates of UP (AHUP) exhibited similar antibacterial activities, but at much lower concentrations of 37.5-300 µg/mL. Since AHUP comprises hydroxycinnamic acids such as caffeic acid, p-coumaric acid, and ferulic acid, the antibacterial activities of each of these acids were examined. Our study shows that the phenolic compounds in P. mume other than citric acid contribute to its antimicrobial activity against enterobacteria in the digestive tract.

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Section: Involvement Of Citric Acid In Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial Activity of the Phenolic Compounds of Prunus mume against Enterobacteria

Mitani

Ota

Inaba

et al. 2018

Biological & Pharmaceutical Bulletin

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“…Antimicrobial activity has been recognized as the major beneficial effect of organic acids on animal production, although the exact antimicrobial mechanism has not been fully revealed. Several studies indicated that the PM extract was a potential candidate for developing an antimicrobial agent to control or inhibit Helicobacter pylori, associated with gastritis and gastric ulcers (Enomoto et al, 2010) and several pathogenic bacteria (Lee et al, 2003b;Seneviratne et al, 2011). Wang et al (2010) reported that PM showed an inhibitory effect on the growth of Staphylococcus aureus, E. coli, and Psedomonas aeruginosa in vitro study, indicating that organic acids in PM were responsible for antimicrobial actions.…”

Section: Discussionmentioning

confidence: 99%

“…One of feasible reasons for increased pancreatic enzymes in birds fed PM extract was the composition of PM extract, which contained a mixture of organic acids including malic acid, citric acid, succinic acid, lactic acid, etc. (Shim et al, 1989;Lim, 1999;Chen et al, 2006). It has been reported that PM contained 55.94% organic acids, 5.50% crude ash, 4.29% crude protein, 5.85% crude fat and 1.10% polyphenol as dry matter basis (Lim, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…), its common names including Chinese plum and Japanese apricot, has long been used as a traditional medicinal and healthy functional food in China, Japan and Korea to treat enteric disorder or improve digestion in humans (Jee, 1999). A recent study indicated that the Prunus mume (PM) extract was a potential candidate for developing an antimicrobial agent to control or inhibit Helicobacter pylori, associated with gastritis and gastric ulcers (Enomoto et al, 2010) and several pathogenic bacteria (Lee et al, 2003b;Seneviratne et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Plum (Prunus Mume Siebold and Zucc.) Products on Growth Performance, Intestinal Function and Immunity in Broiler Chicks

Jang

Kang

2013

J. Poult. Sci.

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The present study was designed to evaluate whether plum (Prunus mume Siebold and Zucc., PM) products affect growth performance, digestive enzymes, enteric microflora and inflammatory cytokines in growing broiler chicks. A total of one hundred twenty eight, 3-d-old broiler chicks were assigned to a basal diet (CON) and a basal diet supplemented with antibiotics (ANT), freeze-dried Prunus mume powder (PMP, 0.25%) and Prunus mume extract (PME, 0.125%) until 35 days of age. Throughout the entire feeding period (3-35 days), there were no differences in body weight, feed intake, total gain and feed to gain ratio among the birds fed the basal diet and those fed the diet supplemented with antibiotics, either PM powder or extract. The specific activities of pancreatic α-amylase and trypsin significantly increased (P＜0.05) in birds fed the PME diet compared with those fed the CON and ANT diets. However, the specific activities of intestinal hydrolases such as maltase, sucrase and leucine aminopeptidase were not affected by the dietary groups. The colony forming units (CFU) of E. coli in the digesta of ileo-cecum in the PMP and PME groups were similar to those in the ANT group. The CFU of lactobacilli in the PMP and PME groups was significantly greater (P＜0.05) than that in the ANT group, although there was no difference between the PMP and PME groups. The mRNA expression of splenic IL-1β and IL-6, pro-inflammatory cytokines, was significantly higher (P＜0.05) in the PME group than in the CON group without affecting thymic cytokines. In summary, the dietary PM extract showed beneficial effects on pancreatic digestive enzymes, enteric microflora population and inflammatory cytokine mRNA expression, suggesting that PM extract might be a potential candidate as an alternative to antibiotics in birds.

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“…The cytotoxicity of plants, extracts, fractions, and isolated compounds has often been reported to depend on concentration and contact time (Gursoy et al, 2009;Vargas et al, 2010). This toxicity is many times moderate (More et al, 2008;Signoretto et al, 2011) or even uncapable of causing deleterious effects (Seneviratne et al, 2011), but further drug safety assessment is essential before diterpenes of the pimarane type are introduced into the market for use in humans.…”

Section: Discussionmentioning

confidence: 99%

Diterpenes of the pimarane type isolated from Viguiera arenaria: Promising in vitro biological potential as therapeutic agents for endodontics

Marangoni¹,

Moraes²,

Utrera³

et al. 2018

J. Pharmacognosy Phytother.

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Viguiera arenaria, family Asteraceae, is a plant that contains diterpenoids, which make this species potentially applicable in endodontics. More specifically, V. arenaria contains diterpenes of the pimarane type, which display various classic biological activities. This study evaluates the antibiofilm activity, the time-kill curve, and the inhibitory concentration index of diterpenes of the pimarane type (entpimara-8(14),15-dien-19-oic acid, ent-8(14),15-pimaradien-3β-ol, and ent-8(14),15-pimaradien-3β-19-oic acid sodium salt, designated diterpenes I, II, and III, respectively) toward nine anaerobic bacteria commonly found in endodontic infections; this study also assesses the cytotoxic activity of these diterpenes against human fibroblasts. According to the antibiofilm assay, diterpenes I, II, and III inhibit at least 50% of all the bacteria. On the basis of the time-kill curve experiments, the behavior of these diterpenes depends on the tested bacteria, diterpene concentration, and microorganism sensitivity. Synergism of diterpenes I and II with chlorhexidine (CDH) was higher against P. gingivalis (clinical isolate) and Aggregatibacter actinomycetemcomitans (ATCC). As for diterpene III, synergism with CDH is higher against P. micros. As revealed by the XTT assay, none of the diterpenes of the pimarane type tested here are cytotoxic. Hence, diterpenes I, II, and III are promising biomolecules and may provide therapeutic solutions in the field of endodontics.

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Prunus mume extract exhibits antimicrobial activity against pathogenic oral bacteria

Abstract: Prunus mume extract may be a potential candidate for developing an oral antimicrobial agent to control or prevent dental diseases associated with oral pathogenic bacteria.

Cited by 32 publications

References 16 publications

Antimicrobial Activity of the Phenolic Compounds of <i>Prunus mume</i> against Enterobacteria

Antimicrobial Activity of the Phenolic Compounds of <i>Prunus mume</i> against Enterobacteria

Influence of Plum (<i>Prunus Mume </i>Siebold and Zucc.) Products on Growth Performance, Intestinal Function and Immunity in Broiler Chicks

Diterpenes of the pimarane type isolated from Viguiera arenaria: Promising in vitro biological potential as therapeutic agents for endodontics

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