Prunus armeniaca effects on expression of genes related to apoptosis in human breast cancer cells

Abstract

Background: Apoptosis inhibition depends on the balance between expression of some regulatory genes including Bax and c-FLIP, despite breast cancer has long been associated with disturbances in apoptosis regulation. The objective of present study was to determine the expression levels of Bax and c-FLIP mRNAs in 1 μg sample of total RNA obtained from MCF7 and MDA-MB-231 human breast cancer cells treated with Prunus armeniaca extract. Methods: Prunus armeniaca effect was measured by MTT assay with different drug concentrations (0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5 mg/mL) at different incubation times (24, 48 and 72 h). Expression of Bax and c-FLIP mRNA levels was determined by qRT-PCR technique. Results: The results of this study indicated that Prunus armeniaca significantly inhibited proliferation of cells in concentration-dependent manner in all the time of incubation (P < 0.05). Our findings also showed that the expression level of Bax and c-FLIP genes were increased in untreat group compared with control group in both of cancer cells at all the time (P < 0.001). Prunus armeniaca reduced the expression level of Bax and c-FLIP genes cancer cells as a timedependence manner with respect to the untreat group significantly (P < 0.001). Conclusion: The expression level of Bax and c-FLIP genes were significantly decreased under treatment of Prunus armeniaca extract and it may be helpful in the prevention and cure of breast cancer in usage of herbal remedies.

Efficacy of Prunus Armeniaca on Oral Squamous cell carcinoma Cell Line: An Ex- Vivo study

“…Consequently they suspected that PA could be related to anti-apoptotic genes inhibition in Human breast cancer. (Mahmoudi et al, 2019) An animal study performed on rabbits contradicted our results and stated that the apricot seeds contents, amygdalin, might have a potential risk on animal health as it caused microscopic structure changes in their livers. Nevertheless, this study couldn't verify the toxic effect in relation to the highest dose supplied (Kolesárová et al, 2020) On the other hand, Cassiem & Kock in 2019 reported that there was an intra S-phase block in the cell cycle of human colon cancer cells in vitro after been treated with South African kernel extract.…”

“…Hence recent cancer research is directed towards dysregulation of apoptosis being the beginning of carcinogenesis. Number of studies revealed the signi cant role that apoptosis play in tumorigeneses (Mahmoudi et al, 2019) Minaiyan et al, performed a study on rats, where they induced ulcerative colitis and treated it with PA comparing it with prednisolone as the standard treatment. Their results showed improve effect in the PA group especially with those treated intraperitoneal than those treated orally.…”

“…The therapeutic actions of PA is important but still under investigation as its not supported by enough evidence-based studies. Thus, the aim of this study is to evaluating the effect of PA extract on OSCC (Mahmoudi et al, 2019)…”

Efficacy of Prunus Armeniaca on Oral Squamous cell carcinoma Cell Line: An In-Vitro Analysis of a Cell Line

“…There is not enough evidence to support the therapeutic effects of PA, thus this area is still being researched. Therefore, the purpose of this research is to assess PA extract's impact on OSCC (Mahmoudi et al, 2019).…”

Anticancer Potential and Other Pharmacological Properties of Prunus armeniaca L.: An Updated Overview

“…An extract from apricots significantly inhibited the proliferation of MCF-7, HDF, and MDA-MB-231 human breast cancer cells in a concentration-dependent manner after 24, 48, and 72 h. The IC 50 values of this extract against MCF7, HDF, and MDA-MB-231 cells after 72 h were 0.5, 1.51, and 0.48 mg/mL, respectively. The expression levels of Bax and c-FLIP regulatory genes, obtained from the total RNA of MCF-7 and MDA-MB-231 cells, were reduced in the presence of the extract in a time-dependent manner [ 87 ].…”