PRUNE2 inhibits progression of colorectal cancer<i> in vitro</i> and <i>in vivo</i>

Li, Ting; Huang, Silin; Yan, Wei; Zhang, Yu; Guo, Qiang

doi:10.3892/etm.2021.11092

Cited by 2 publications

(3 citation statements)

References 27 publications

(45 reference statements)

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“…Interestingly, we previously demonstrated that PRUNE2 has the same functions as FOXF2 in CRC, and the survival prognosis of patients with low expression of PRUNE2 was poor. 42 Thus, we speculated that FOXF2 may regulate PRUNE2 and that this regulatory correlation may play an important role in CRC. Herein, we identified PRUNE2 as a novel transcriptional target of FOXF2 and demonstrated that FOXF2 regulated the proliferation and progression of CRC by positively regulating PRUNE2 transcription.…”

Section: Discussionmentioning

confidence: 99%

“… 36 A previous study demonstrated that PRUNE2 decreased cell survival, proliferation, invasion, and tumorigenicity and promoted apoptosis, suggesting that PRUNE2 functions as a tumor-suppressive gene in CRC. 42 However, the role of PRUNE2 and its mechanism in CRC are still unclear. Taking the above into consideration, we assumed that PRUEN2 mediates the inhibitory function of FOXF2 in the development and pathogenesis of CRC.…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression of PRUNE2 significantly inhibited the proliferation, migration, and invasion of CRC cells and significantly inhibited the growth of CRC cells in nude mice, suggesting that PRUNE2 inhibits the growth of CRC in vivo and in vitro. 42 However, the association between FOXF2 and PRUNE2 expression in CRC is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer

Huang

Yan

et al. 2022

Technol Cancer Res Treat

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Background: Forkhead box F2, a member of the Forkhead box transcription factor superfamily, plays an important role in several types of cancer. However, the mechanisms of Forkhead box F2 in the progression of colorectal cancer remain unclear. PRUNE2 is closely associated with prostate cancer, neuroblastoma, glioblastoma, and melanoma. The relationship between Forkhead box F2 and PRUNE2 in colorectal cancer remains unknown. Method: We investigated the effects of Forkhead box F2 upregulation on colorectal cancer cell behavior in vitro using Cell Counting Kit-8, colony formation, flow cytometry, Transwell, reverse transcription quantitative polymerase chain reaction and Western blot analyses. Nude mouse xenografts were established to investigate the effect of Forkhead box F2 upregulation on the growth of colorectal cancer cells. Dual-luciferase reporter assays were performed to confirm the Forkhead box F2 regulation of PRUNE2 transcription. A series of in vitro assays was performed in cells with Forkhead box F2 upregulation and PRUNE2 knockdown to elucidate the function and regulatory effects of Forkhead box F2 on PRUNE2 transcription in colorectal cancer. Results: Forkhead box F2 was downregulated in colorectal cancer tissues compared with adjacent tissues. Forkhead box F2 overexpression significantly suppressed the proliferation and invasion of colorectal cancer cells in vitro and in vivo. Moreover, Forkhead box F2 directly targeted PRUNE2 to promote its transcription in colorectal cancer cells. Furthermore, PRUNE2 mediated the Forkhead box F2-regulated proliferation and invasion of colorectal cancer cells. Additionally, we demonstrated a significant positive correlation between Forkhead box F2 and PRUNE2 mRNA levels in colorectal cancer tissues. Conclusion: These results indicated that Forkhead box F2 and PRUNE2 in combination may serve as a prognostic biomarker for colorectal cancer and that Forkhead box F2 upregulation inhibits the proliferation and invasion of colorectal cancer cells by upregulating PRUNE2.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer

Huang

Yan

et al. 2022

Technol Cancer Res Treat

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Novel PRUNE2 Germline Mutations in Aggressive and Benign Parathyroid Neoplasms

Storvall

Ryhänen

Karhu

et al. 2023

Cancers

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Parathyroid tumors are mostly sporadic but can also occur in familial forms, including different kinds of genetic syndromes with varying phenotypes and penetrance. Recently, somatic mutations of the tumor suppressor gene PRUNE2 were found to be frequent in parathyroid cancer (PC). The germline mutation status of PRUNE2 was investigated in a large cohort of patients with parathyroid tumors from the genetically homogenous Finnish population, 15 of which had PC, 16 atypical parathyroid tumors (APT), and 6 benign parathyroid adenomas (PA). Mutations in previously established hyperparathyroidism-related genes were screened with a targeted gene panel analysis. Nine PRUNE2 germline mutations with a minor allele frequency (MAF) of <0.05 were found in our cohort. Five of these were predicted to be potentially damaging and were identified in two patients with PC, two with APT, and three with PA. The mutational status was not associated with the tumor group nor related to the clinical picture or severity of the disease. Still, the frequent finding of rare germline mutations of PRUNE2 may point to the gene playing a role in the pathogenesis of parathyroid neoplasms.

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PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo

Cited by 2 publications

References 27 publications

FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer

FOXF2 Regulates PRUNE2 Transcription in the Pathogenesis of Colorectal Cancer

Novel PRUNE2 Germline Mutations in Aggressive and Benign Parathyroid Neoplasms

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