Prune supplementation for 12 months alters the gut microbiome in postmenopausal women

Simpson, Abigayle M.R.; Souza, Mary Jane De; Damani, Janhavi; Rogers, Connie J.; Williams, Nancy I.; Weaver, Connie M.; Ferruzzi, Mario G.; Chadwick-Corbin, Sydney; Nakatsu, Cindy H.

doi:10.1039/d2fo02273g

Cited by 6 publications

(4 citation statements)

References 55 publications

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“…Our results show that DP supplementation leads to drastic shifts in gut microbiota composition and that specific shifts in microbial community composition are strongly linked with trabecular bone health. Previous studies have investigated the effect of DP supplementation on gut microbiota composition in rodent models of primary osteoporosis and in post-menopausal women ( Simpson et al, 2022 ; Smith et al, 2022 ). Prune supplementation led to drastic shifts in gut microbiota composition in both.…”

Section: Discussionmentioning

confidence: 99%

“…We did not identify families S24-7 or Coriobacteriaceae ; these may have been present at very low abundances or may not have been present within our vivarium. In the human study, the women whose diets were supplemented with 50 g of prunes per day had increases in members of the Lachnospiraceae family, genus Blautia , and genus Anaerostipes and a reduction in order Oscillospirales ( Simpson et al, 2022 ). We observe similar responses in family Lachnospiraceae but do not observe any differences in Blautia or Oscillospirales (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…Postmenopausal women that ingested 50-100 g of prunes per day for 6-12 months showed attenuated bone loss (Arjmandi et al, 2002(Arjmandi et al, , 2017Hooshmand et al, 2011Hooshmand et al, , 2016, with more modest effects in men (Hooshmand et al, 2011(Hooshmand et al, , 2014(Hooshmand et al, , 2016(Hooshmand et al, , 2021Gaffen et al, 2019;Fajardo et al, 2020;Munoz et al, 2021). In addition, consumption of 50 g (~6 prunes) per day for 1 year not only prevented further reductions in total hip bone mineral density (BMD) in postmenopausal women but was also associated with significant changes in gut microbiota composition (De Souza et al, 2022;Simpson et al, 2022). Taken together, there is significant evidence that DP supplementation causes shifts in gut microbiota composition and benefits bone health in both humans and animal models, especially in the context of primary osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

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Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice

Chargo,

Neugebauer,

Guzior

et al. 2024

Front. Cell Dev. Biol.

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Glucocorticoid-induced osteoporosis (GIO) is a significant side effect of prolonged glucocorticoid (GC) treatment. Chronic GC treatment also leads to trabecular bone loss and gut microbiota dysbiosis in mice. The gut dysbiosis is mechanistically linked to GIO, which indicates that the microbiota can be targeted to prevent GIO. Prunes, a dried fruit and prebiotic, have emerged in the literature as an effective treatment for sex-steroid deficiency induced osteoporosis (primary osteoporosis). Prunes also significantly alter the composition of the gut microbiota in both rodent models and human studies. Therefore, we tested if dietary prune (DP) supplementation could prevent GC-induced bone loss and affect microbiota composition in an established model of GIO. Sixteen-week-old, skeletally mature, female C57BL/6J mice were treated with a subcutaneous 5 mg placebo or prednisolone pellet for 8 weeks and fed an AIN-93M control diet or a diet modified to include 5, 15, or 25% (w/w) dried California prune powder. As expected, GC treated mice developed significant trabecular bone loss in the distal femur. More importantly, as little as 5% DP supplementation effectively prevented trabecular bone loss. Further, dose dependent increases in trabecular bone volume fraction were observed in GC + 15% and GC + 25% DP mice. Amazingly, in the placebo (non-GC treated) groups, 25% DP supplementation caused a ∼3-fold increase in distal femur trabecular bone volume fraction; this sizable bone response has not been previously observed in healthy mice with gut targeted natural treatments. Along with the striking effect on bone health, GC treatment and 25% DP supplementation led to drastic shifts in gut microbiota composition and several specific changes are strongly associated with bone health. Taken together, these results are the first to demonstrate that DP supplementation effectively prevents the negative effects of prolonged GC therapy on trabecular bone health and strongly associates with shifts in the composition of the gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice

Chargo,

Neugebauer,

Guzior

et al. 2024

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

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“…The total hip BMD 1.1% decrease in the controls was blunted by prunes ingestion. In the prune consumers group, microbiota was modified with an increase in Lachnospiraceae , together with higher hippuric acids production and lower activated monocytes [ 46 ].…”

Section: Gut Microbiotamentioning

confidence: 99%

Bone health: biology and nutrition

Rizzoli,

Chevalley

2023

Current Opinion in Clinical Nutrition &Amp; Metabolic Care

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Purpose of review Recent findings in the influence of dietary patterns, dairy products, beverages and microbiota composition and function on bone health are reviewed and discussed. Recent findings Evidence is accumulating on the increased risk of fracture in individuals following a vegan diet. Meta-analysis of randomized controlled trials indicates a favourable, though of low amplitude, effect of dairy products on bone mass accrual during childhood and adolescence. Though mostly based on results from observational studies, it seems that dairy product consumption, particularly fermented dairy products, is associated with a lower risk of hip fracture. Regular green tea drinkers may have a lower fracture risk than tea abstainers. Magnesium intake is beneficial for bone health. Prune supplements prevents bone loss in untreated postmenopausal women. This seems to be associated with modification of gut microbiota. Summary This information should help the medical practitioners facing questions from their patients on how to protect bone health through nutrition.

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Unveiling metabotype clustering in resveratrol, daidzein, and ellagic acid metabolism: Prevalence, associated gut microbiomes, and their distinctive microbial networks

Eduardo Iglesias-Aguirre,

Romo-Vaquero,

Victoria Selma

et al. 2023

Food Research International

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Prune supplementation for 12 months alters the gut microbiome in postmenopausal women

Abstract: Prunes have health benefits, particularly in postmenopausal women. It is likely that the gut microbiome mediates some of these effects, but its exact role remains to be elucidated. This study...

Cited by 6 publications

References 55 publications

Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice

Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice

Bone health: biology and nutrition

Unveiling metabotype clustering in resveratrol, daidzein, and ellagic acid metabolism: Prevalence, associated gut microbiomes, and their distinctive microbial networks

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