Pruebas de aliento en el diagnóstico de enfermedades digestivas

Stable isotope breath tests in clinical medicine: a review

“…In this review, I wish to concentrate on research in the medical field dedicated to 13 C-labelled carbon dioxide in exhaled air following ingestion of 13 C-labelled substrates. Stable isotope 13 C-labelled compounds have been widely used as diagnostic probes in research laboratories for over 30 years.…”

“…In this review, I wish to concentrate on research in the medical field dedicated to 13 C-labelled carbon dioxide in exhaled air following ingestion of 13 C-labelled substrates. Stable isotope 13 C-labelled compounds have been widely used as diagnostic probes in research laboratories for over 30 years. In 1973, 13 C-glucose was the substrate in the first report on the use of a naturally occurring 13 C compound for a metabolic study in humans [3].…”

“…Stable isotope 13 C-labelled compounds have been widely used as diagnostic probes in research laboratories for over 30 years. In 1973, 13 C-glucose was the substrate in the first report on the use of a naturally occurring 13 C compound for a metabolic study in humans [3]. Prior to this report, similar investigations were conducted using the radioactive isotope 14 C instead of the stable isotope 13 C. Subsequent to the wide availability of low-cost 13 C-substrates and the development of non-dispersive isotope selective IR spectrometry (NDIRS), research groups from around the world have increasingly used these tracers in nutritional, medicinal and veterinary research.…”

“…In 1973, 13 C-glucose was the substrate in the first report on the use of a naturally occurring 13 C compound for a metabolic study in humans [3]. Prior to this report, similar investigations were conducted using the radioactive isotope 14 C instead of the stable isotope 13 C. Subsequent to the wide availability of low-cost 13 C-substrates and the development of non-dispersive isotope selective IR spectrometry (NDIRS), research groups from around the world have increasingly used these tracers in nutritional, medicinal and veterinary research. These diagnostic 13 C-stable isotope probes are now being expanded in their scope, to provide precise evaluations of the presence or absence of etiologically significant changes in metabolism due to a specific disease or the lack of a specific enzyme.…”

Evaluación de un test del aliento con carbono 13 para el diagnóstico de Helicobacter pylori Test del aliento con carbono 13 para H. pylori

“…El TC13 se basa en la capacidad de producción de ureasa del Hp, enzima que permite hidrolizar la urea marcada con carbono 13 contenida en el kit diagnóstico, desdoblándola en amonio y dióxido de carbono marcado. Este último se absorbe a través de la mucosa del estómago y llega al torrente sanguíneo hasta llegar a los pulmones donde se excreta a través del aire espirado y es ahí donde es detectado por un espectrómetro de masas de isótopos estables (15)(16)(17)(18) . Debido a la alta prevalencia de infección por Hp, riesgo de cronicidad y complicaciones irreversibles que ocasiona, es imprescindible la confirmación del diagnóstico y erradicación de la infección después de un tratamiento adecuado; siendo ventajosa la implementación de un test no invasivo, costoefectivo, fácilmente disponible y rápido dentro del protocolo habitual en nuestro medio (19) .…”