PRR34‐AS1 overexpression promotes protection of propofol pretreatment against ischemia/reperfusion injury in a mouse model after total knee arthroplasty via blockade of the JAK1‐dependent JAK‐STAT signaling pathway

Fang, Hua; Zhang, Fangxiang; Li, Huafeng; Yang, Miao; Ren, Lei-Ming; Wang, Rurong; Wang, Quan-Yun; Zheng, Pengcheng; Zhang, Jianping

doi:10.1002/jcp.29158

Cited by 7 publications

(7 citation statements)

References 43 publications

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“…The MDA levels from B-HH to O-HH showed a MD of 1.80 nmol/L and 95% CI of 1.53 to 2.07 nmol/L (P<0.05). The ALT and AST levels had a MD of 8.24 IU/L and −11.73 IU/L, respectively, and the 95% CI was 6.43 to 10.06 IU/L and −14.04 to −9.43 IU/L, respectively (P<0.05) (15). The funnel charts of various indicators revealed that some of the circles were concentrated on the midline and were basically symmetrical with the midline, suggesting that the research accuracy was high and there was no bias in publication (16).…”

Section: Discussionmentioning

confidence: 96%

A meta-analysis and systematic review of propofol on liver ischemia-reperfusion injury protection during hepatocellular carcinoma anesthesia surgery

Feng¹,

Qian²,

Chen³

2021

Ann Palliat Med

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Background: At present, liver cancer deaths of China account for about half of the global liver cancer deaths. The most common physiological change in anesthesia surgery for liver cancer is liver ischemiareperfusion injury (LIRI).Methods: The Chinese and English medical databases were searched using a combination of the following search terms: "propofol", "liver cancer", "anesthesia surgery", and "ischemia reperfusion injury" in Chinese or English language, respectively. The articles taking patients received propofol intravenous anesthesia surgery for LIRI in the experimental group and patients received intravenous anesthesia with non-propofol drugs for LIRI in the control group were searched. Rev Man 5.3 software was used for meta-analysis.Results: A total of 18 articles were included, and most were considered to have low-risk bias (that is, medium-and high-quality publications). The meta-analysis results indicated that the superoxide dismutase (SOD) levels from the blockage of the hepatic hilum (B-HH) to the 15-minute opening of the hepatic hilum

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Section: Discussionmentioning

confidence: 96%

A meta-analysis and systematic review of propofol on liver ischemia-reperfusion injury protection during hepatocellular carcinoma anesthesia surgery

Feng¹,

Qian²,

Chen³

2021

Ann Palliat Med

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“…It has been previously reported that JAK/STAT signaling is involved in several important cellular processes, including cell proliferation, migration, apoptosis and inflammation (41)(42)(43). A number of studies have suggested that JAK/STAT signaling is involved in the pathogenesis of tissue and organ I/R injury, including intestinal I/R injury (22)(23)(24). By inactivating JAK/STAT3 signaling, dexmedetomidine was shown to inhibit the apoptosis of astrocytes induced by OGD/R (44).…”

Section: Discussionmentioning

confidence: 99%

“…JAK1 and JAK3 can activate STATs by phosphorylation (21). An increasing number of studies have suggested that the JAK/STAT signaling is involved in the pathogenesis of tissue and organ I/R injury, including intestinal I/R injury (22)(23)(24). Therefore, the present study aimed to investigate the role of Tofa and its possible regulatory effects on the JAK/STAT signaling pathway in intestinal I/R injury.…”

Section: Introductionmentioning

confidence: 99%

Tofacitinib protects intestinal epithelial cells against oxygen‑glucose deprivation/reoxygenation injury by inhibiting the JAK/STAT3 signaling pathway

Yang

Xie

2021

Exp Ther Med

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The present study aimed to investigate the role and potential mechanism of action of tofacitinib (Tofa) in intestinal ischemia/reperfusion (I/R) injury. The normal rat small intestine epithelial cell line, IEC-6, was used to establish an I/R injury model by inducing oxygen-glucose deprivation/reoxygenation (OGD/R). Cells were divided into the following five groups: Control, OGD/R, OGD/R with 50, 100 and 200 nM Tofa. Following Tofa administration, cell viability was measured using Cell Counting Kit-8 assay and a lactate dehydrogenase detection kit. The expression levels of cell apoptosis-related proteins, Bcl-2, cleaved-caspase-3 and cleaved-caspase-9 were detected using western blot analysis. Additionally, the levels of oxidative stress-related markers, such as reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD), and inflammatory cytokines, TNF-α, IL-6 and IL-1β were assessed using the colorimetric method. Western blot analysis was also used to measure the expression levels of the Janus kinase (JAK)/STAT3 signaling pathway-related proteins, including phosphorylated (p)-JAK1, p-JAK3 and p-STAT3. Subsequently, colivelin, an agonist of the JAK/STAT3 pathway, was used to investigate whether the effects of Tofa on intestinal I/R injury were mediated by this signaling pathway. The results showed that Tofa dose-dependently elevated cell viability compared with that in the OGD/R group. By contrast, Tofa attenuated cell apoptosis, which was coupled with upregulated Bcl-2 expression, downregulated cleaved-caspase-3 and downregulated cleaved-caspase-9 levels, in OGD/R-induced IEC-6 cells. Furthermore, the contents of ROS and MDA were significantly increased following exposure to OGD/R, which were accompanied by the decreased activity of SOD. These effects were reversed following cell treatment with Tofa. Consistently, Tofa intervention reduced the secretion levels of TNF-α, IL-6 and IL-1β in a dose-dependent manner. Additionally, Tofa markedly downregulated the phosphorylation levels of JAK1, JAK3 and STAT3 in OGD/R-induced IEC-6 cells. However, treatment with colivelin markedly reversed the inhibitory effects of Tofa on cell viability, cell apoptosis, oxidative stress and inflammation. Overall, the findings of the present study suggested that Tofa could protect against intestinal I/R injury by inhibiting the JAK/STAT3 signaling pathway, which may hold promise as a therapeutic agent for intestinal I/R injury.

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“…PRR34-AS1 (PRR34 antisense RNA 1) is an RNA gene affiliated with the long noncoding RNA (lncRNA) class ( Zhao et al, 2017 ; Tao et al, 2019 ). It has been reported that PRR34-AS1 is closely associated with the early tumor recurrence in bile duct cancer ( Fang et al, 2020 ), and it has been proved to potentially relieve ischemic reperfusion injury after total knee arthroplasty in mice by diminishing apoptosis and enhancing cell proliferation of chondrocyte in vitro ( Fang et al, 2020 ). In our research, the expression level of PRR34-AS1 was negatively correlated with nearly all immune cells and its overexpression indicated a higher risk to be diagnosed as AR patients, which may suggest a novel insight into the relationship between gene and cells.…”

Section: Discussionmentioning

confidence: 99%

A Three-Gene Peripheral Blood Potential Diagnosis Signature for Acute Rejection in Renal Transplantation

Wang

Zhang

2021

Front. Mol. Biosci.

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Background: Acute rejection (AR) remains a major issue that negatively impacts long-term allograft survival in renal transplantation. The current study aims to apply machine learning methods to develop a non-invasive diagnostic test for AR based on gene signature in peripheral blood.Methods: We collected blood gene expression profiles of 251 renal transplant patients with biopsy-proven renal status from three independent cohorts in the Gene Expression Omnibus database. After differential expression analysis and machine learning algorithms, selected biomarkers were applied to the least absolute shrinkage and selection operator (LASSO) logistic regression to construct a diagnostic model in the training cohort. The diagnostic ability of the model was further tested in validation cohorts. Gene set enrichment analysis and immune cell assessment were also conducted for further investigation.Results: A novel diagnostic model based on three genes (TSEN15, CAPRIN1 and PRR34-AS1) was constructed in the training cohort (AUC = 0.968) and successfully verified in the validation cohort (AUC = 0.925) with high accuracy. Moreover, the diagnostic model also showed a promising value in discriminating T cell-mediated rejection (TCMR) (AUC = 0.786). Functional enrichment analysis and immune cell evaluation demonstrated that the AR model was significantly correlated with adaptive immunity, especially T cell subsets and dendritic cells.Conclusion: We identified and validated a novel three-gene diagnostic model with high accuracy for AR in renal transplant patients, and the model also performed well in distinguishing TCMR. The current study provided a promising tool to be used as a precise and cost-effective non-invasive test in clinical practice.

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PRR34‐AS1 overexpression promotes protection of propofol pretreatment against ischemia/reperfusion injury in a mouse model after total knee arthroplasty via blockade of the JAK1‐dependent JAK‐STAT signaling pathway

Cited by 7 publications

References 43 publications

A meta-analysis and systematic review of propofol on liver ischemia-reperfusion injury protection during hepatocellular carcinoma anesthesia surgery

A meta-analysis and systematic review of propofol on liver ischemia-reperfusion injury protection during hepatocellular carcinoma anesthesia surgery

Tofacitinib protects intestinal epithelial cells against oxygen‑glucose deprivation/reoxygenation injury by inhibiting the JAK/STAT3 signaling pathway

A Three-Gene Peripheral Blood Potential Diagnosis Signature for Acute Rejection in Renal Transplantation

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