PRR11 and SKA2 promote the proliferation, migration and invasion of esophageal carcinoma cells

Abstract

Proline-rich protein 11 (PRR11) together with its upstream adjacent gene, spindle and kinetochore associated 2 (SKA2), represent a classic, head-to-head gene pair. The role of the PRR11 and SKA2 gene pair has been described in various types of cancer, including breast cancer, non-small cell lung cancer, hepatocellular carcinoma and ovarian carcinoma. However, its role in esophageal carcinoma (ESCC) remains unclear. The mRNA expression levels of PRR11 and SKA2 were examined in ESCC surgical specimens. In addition, the role of PRR11 and SKA2 in the proliferation and migratory and invasive capacities of EC9706 and EC109 cell lines was examined. The results from the present study demonstrated that PRR11 and SKA2 expression levels were upregulated in ESCC tissues compared with adjacent normal tissues. Furthermore, PRRl1 and SKA2 knockdown significantly inhibited the proliferation and migratory and invasive capacities of ESCC cells. Conversely, PRRl1 and SKA2 overexpression significantly promoted the proliferation and migratory and invasive capacities of ESCC cell lines via activation of the AKT signaling pathway and certain markers of epithelial-mesenchymal transition, including Snail and N-cadherin. The results from the present study suggested that the PRR11 and SKA2 gene pair may represent a potential target in the diagnosis and treatment of ESCC.

Overexpression of SKA3 correlates with poor prognosis in female early breast cancer

“…Mitotic abnormalities are characteristic of most tumors ( Suzuki et al, 2018 ). PRR11 and SKA2 gene pair are overexpressed in breast cancer and esophageal carcinoma ( Chen et al, 2020 ; Wang et al, 2019 ), which accelerate proliferation, migration, and invasive capabilities. SKA2 can mediate the proliferation, migration, and invasion of breast cancer cells through EMT ( Ren et al, 2019 ).…”

PRR11 in Malignancies: Biological Activities and Targeted Therapies

“…Analysis of ESCC patients from the GEO database has verified the oncogenic role of PRR11 in ESCC tumorigenesis [ 29 ]. Relatively higher expression levels of PRR11 are also detected in 12 ESCC cell lines and 38 ESCC tissues than in 2 normal esophageal epithelial cells (NEECs) and the matched adjacent non-tumor tissues [ 4 , 15 ]. Further investigation revealed that PRR11 dramatically facilitated cellular proliferation and the migratory and invasive capacities of ESCC cells targeting Akt and EMT signaling via collaboration with SKA2 [ 4 ].…”

“…The PRR11 mRNA is 2408 bp in length, containing an open reading frame encoding 360 amino acids [ 2 ]. At the protein level, PRR11 is composed of a nuclear localization signal (NLS), two proline rich regions (PRs) and a zinc finger domain (ZFD) [ 4 ] ( Figure 1 a,b).…”

SKA1/2/3 is a biomarker of poor prognosis in human hepatocellular carcinoma

“…The SKA2 expression level is upregulated in esophageal carcinoma tissues compared with the adjacent normal tissues. Through enhancing the AKT signaling pathway activity and elevating the expression of certain epithelial–mesenchymal transition-associated markers, such as N-cadherin and Snail, SKA2 could significantly participated in the regulation of esophageal carcinoma cells proliferation, migration, and invasion ( 8 ). SKA3 is a target of KIRP and predicts a poor prognosis due to its effects on the RAS/MAPK, PI3K/Akt, hormone estrogen receptor, hormone androgen receptor, DNA damage, and cell cycle ( 9 ).…”